Macro-Architectures in Spinal Cord Scaffold Implants Influence Regeneration
dc.contributor.author | Wong, Darice Yukfun | en_US |
dc.contributor.author | Leveque, Jean-Christophe | en_US |
dc.contributor.author | Brumblay, Hunter | en_US |
dc.contributor.author | Krebsbach, Paul H. | en_US |
dc.contributor.author | Hollister, Scott J. | en_US |
dc.contributor.author | LaMarca, Frank | en_US |
dc.date.accessioned | 2009-07-10T19:06:51Z | |
dc.date.available | 2009-07-10T19:06:51Z | |
dc.date.issued | 2008-08-01 | en_US |
dc.identifier.citation | Wong, Darice Y.; Leveque, Jean-Christophe; Brumblay, Hunter; Krebsbach, Paul H.; Hollister, Scott J.; LaMarca, Frank (2008). "Macro-Architectures in Spinal Cord Scaffold Implants Influence Regeneration." Journal of Neurotrauma 25(8): 1027-1037 <http://hdl.handle.net/2027.42/63279> | en_US |
dc.identifier.uri | https://hdl.handle.net/2027.42/63279 | |
dc.identifier.uri | http://www.ncbi.nlm.nih.gov/sites/entrez?cmd=retrieve&db=pubmed&list_uids=18721107&dopt=citation | en_US |
dc.description.abstract | Abstract Biomaterial scaffold architecture has not been investigated as a tunable source of influence on spinal cord regeneration. This study compared regeneration in a transected spinal cord within various designed-macro-architecture scaffolds to determine if these architectures alone could enhance regeneration. Three-dimensional (3-D) designs were created and molds were built on a 3-D printer. Salt-leached porous poly(ε-caprolactone) was cast in five different macro-architectures: cylinder, tube, channel, open-path with core, and open-path without core. The two open-path designs were created in this experiment to compare different supportive aspects of architecture provided by scaffolds and their influence on regeneration. Rats received T8 transections and implanted scaffolds for 1 and 3 months. Overall morphology and orientation of sections were characterized by H&E, luxol fast blue, and cresyl violet staining. Borders between intact gray matter and non-regenerated defect were observed from GFAP immunolabeling. Nerve fibers and regenerating axons were identified with Tuj-1 immunolabeling. The open-path designs allowed extension of myelinated fibers along the length of the defect both exterior to and inside the scaffolds and maintained their original defect length up to 3 months. In contrast, the cylinder, tube, and channel implants had a doubling of defect length from secondary damage and large scar and cyst formation with no neural tissue bridging. The open-path scaffold architectures enhanced spinal cord regeneration compared to the three other designs without the use of biological factors. | en_US |
dc.format.extent | 928981 bytes | |
dc.format.extent | 2489 bytes | |
dc.format.mimetype | application/pdf | |
dc.format.mimetype | text/plain | |
dc.publisher | Mary Ann Liebert, Inc., publishers | en_US |
dc.title | Macro-Architectures in Spinal Cord Scaffold Implants Influence Regeneration | en_US |
dc.type | Article | en_US |
dc.subject.hlbtoplevel | Health Sciences | en_US |
dc.description.peerreviewed | Peer Reviewed | en_US |
dc.identifier.pmid | 18721107 | en_US |
dc.description.bitstreamurl | http://deepblue.lib.umich.edu/bitstream/2027.42/63279/1/neu.2007.0473.pdf | |
dc.identifier.doi | doi:10.1089/neu.2007.0473 | en_US |
dc.identifier.source | Journal of Neurotrauma | en_US |
dc.identifier.source | Journal of Neurotrauma | en_US |
dc.owningcollname | Interdisciplinary and Peer-Reviewed |
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