Comparative Analysis of a Monoclonal Antibody-Based Streptococcus mutans Detection Method with Selective Culture Assays Using Polymerase Chain Reaction as a Gold Standard
dc.contributor.author | Gu, Fang | en_US |
dc.contributor.author | Qi, Fengxia | en_US |
dc.contributor.author | Anderson, Maxwell H. | en_US |
dc.contributor.author | Shi, Wenyuan | en_US |
dc.date.accessioned | 2009-07-10T19:11:01Z | |
dc.date.available | 2009-07-10T19:11:01Z | |
dc.date.issued | 2006-12-01 | en_US |
dc.identifier.citation | Gu, Fang; Qi, Fengxia; Anderson, Maxwell H.; Shi, Wenyuan (2006). "Comparative Analysis of a Monoclonal Antibody-Based Streptococcus mutans Detection Method with Selective Culture Assays Using Polymerase Chain Reaction as a Gold Standard." Hybridoma 25(6): 372-377 <http://hdl.handle.net/2027.42/63351> | en_US |
dc.identifier.uri | https://hdl.handle.net/2027.42/63351 | |
dc.identifier.uri | http://www.ncbi.nlm.nih.gov/sites/entrez?cmd=retrieve&db=pubmed&list_uids=17204000&dopt=citation | en_US |
dc.description.abstract | The aim of this study was to compare a recently developed monoclonal antibody (MAb)-based salivary Streptococcus mutans detection method with various selective media using polymerase chain reaction (PCR) as the gold standard. Salivary S. mutans cells were enumerated with a MAb-based method, along with three commonly used selective media, mitis-salivarius-bacitracin agar (MSB), trypticase yeast-extract cystine sucrose bacitracin agar (TYCSB), and glucose-sucrose-potassium tellurite-bacitracin (GSTB) agar. Statistical analysis showed no significant correlations between each method. With PCR as the standard, a MAb-based detection method was found to provide the highest sensitivity (91%) and specificity (96%) among these four methods. This study demonstrates that the MAb-based detection method may provide more accurate enumeration of salivary S. mutans than selective media. | en_US |
dc.format.extent | 120300 bytes | |
dc.format.extent | 2489 bytes | |
dc.format.mimetype | application/pdf | |
dc.format.mimetype | text/plain | |
dc.publisher | Mary Ann Liebert, Inc., publishers | en_US |
dc.title | Comparative Analysis of a Monoclonal Antibody-Based Streptococcus mutans Detection Method with Selective Culture Assays Using Polymerase Chain Reaction as a Gold Standard | en_US |
dc.type | Article | en_US |
dc.subject.hlbtoplevel | Health Sciences | en_US |
dc.description.peerreviewed | Peer Reviewed | en_US |
dc.identifier.pmid | 17204000 | en_US |
dc.description.bitstreamurl | http://deepblue.lib.umich.edu/bitstream/2027.42/63351/1/hyb.2006.25.372.pdf | |
dc.identifier.doi | doi:10.1089/hyb.2006.25.372 | en_US |
dc.identifier.source | Hybridoma | en_US |
dc.identifier.source | Hybridoma | en_US |
dc.owningcollname | Interdisciplinary and Peer-Reviewed |
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