Delayed Precursor Cell Markers Expression in Hippocampus following Cold-Induced Cortical Injury in Mice
dc.contributor.author | Nakamura, Takehiro | en_US |
dc.contributor.author | Miyamoto, Osamu | en_US |
dc.contributor.author | Auer, Roland N. | en_US |
dc.contributor.author | Nagao, Seigo | en_US |
dc.contributor.author | Itano, Toshifumi | en_US |
dc.date.accessioned | 2009-07-10T19:12:09Z | |
dc.date.available | 2009-07-10T19:12:09Z | |
dc.date.issued | 2004-12-01 | en_US |
dc.identifier.citation | Nakamura, Takehiro; Miyamoto, Osamu; Auer, Roland N.; Nagao, Seigo; Itano, Toshifumi (2004). "Delayed Precursor Cell Markers Expression in Hippocampus following Cold-Induced Cortical Injury in Mice." Journal of Neurotrauma 21(12): 1747-1755 <http://hdl.handle.net/2027.42/63371> | en_US |
dc.identifier.uri | https://hdl.handle.net/2027.42/63371 | |
dc.identifier.uri | http://www.ncbi.nlm.nih.gov/sites/entrez?cmd=retrieve&db=pubmed&list_uids=15684766&dopt=citation | en_US |
dc.description.abstract | The purpose of this study was to examine the possibility of neuronal remodeling and repair after cold-induced brain injury using immunoassay of nestin and 3CB2 (potential precursor cell markers). Male ddN strain mice were subjected to cold-induced cortical injury. Animals were divided into the following six groups: (1) 1 day after injury, (2) 1 week after injury, (3) 2 weeks after injury, (4) 1 month after injury, (5) sham controls, and (6) normal controls. Western blot analysis (n = 3 in each group) and histological examination (n = 5 in each group) were performed. At 1 day and 1 week after injury, TUNEL-positive cells were observed, while immunoreactivity of nestin and 3CB2 was absent. At 1 month after injury, expression of both nestin and 3CB2 was observed in the ipsilateral hippocampus. Nestin was expressed in GFAP- or 3CB2-positive astrocytes at 1 month after injury, and nestin expression with TUC-4 (immature neuron marker) was present in the hippocampal cell layer. The findings demonstrate delayed nestin expression in both glia and neuronlike cells after brain injury. The present study suggests that the delayed nestin expression in glia and neuron-like cells might be part of the adaptation to injury. | en_US |
dc.format.extent | 409822 bytes | |
dc.format.extent | 2489 bytes | |
dc.format.mimetype | application/pdf | |
dc.format.mimetype | text/plain | |
dc.publisher | Mary Ann Liebert, Inc., publishers | en_US |
dc.title | Delayed Precursor Cell Markers Expression in Hippocampus following Cold-Induced Cortical Injury in Mice | en_US |
dc.type | Article | en_US |
dc.subject.hlbtoplevel | Health Sciences | en_US |
dc.description.peerreviewed | Peer Reviewed | en_US |
dc.identifier.pmid | 15684766 | en_US |
dc.description.bitstreamurl | http://deepblue.lib.umich.edu/bitstream/2027.42/63371/1/neu.2004.21.1747.pdf | |
dc.identifier.doi | doi:10.1089/neu.2004.21.1747 | en_US |
dc.identifier.source | Journal of Neurotrauma | en_US |
dc.identifier.source | Journal of Neurotrauma | en_US |
dc.owningcollname | Interdisciplinary and Peer-Reviewed |
Files in this item
Remediation of Harmful Language
The University of Michigan Library aims to describe library materials in a way that respects the people and communities who create, use, and are represented in our collections. Report harmful or offensive language in catalog records, finding aids, or elsewhere in our collections anonymously through our metadata feedback form. More information at Remediation of Harmful Language.
Accessibility
If you are unable to use this file in its current format, please select the Contact Us link and we can modify it to make it more accessible to you.