Immunogenetic Therapy of Human Melanoma Utilizing Autologous Tumor Cells Transduced to Secrete Granulocyte-Macrophage Colony-Stimulating Factor
dc.contributor.author | Chang, Alfred E. | en_US |
dc.contributor.author | Li, Qiao | en_US |
dc.contributor.author | Bishop, D. Keith | en_US |
dc.contributor.author | Normolle, Daniel P. | en_US |
dc.contributor.author | Redman, Bruce D. | en_US |
dc.contributor.author | Nickoloff, Brian J. | en_US |
dc.date.accessioned | 2009-07-10T19:14:34Z | |
dc.date.available | 2009-07-10T19:14:34Z | |
dc.date.issued | 2000-04-10 | en_US |
dc.identifier.citation | Chang, Alfred E.; Li, Qiao; Bishop, D. Keith; Normolle, Daniel P.; Redman, Bruce D.; Nickoloff, Brian J. (2000). "Immunogenetic Therapy of Human Melanoma Utilizing Autologous Tumor Cells Transduced to Secrete Granulocyte-Macrophage Colony-Stimulating Factor." Human Gene Therapy 11(6): 839-850 <http://hdl.handle.net/2027.42/63414> | en_US |
dc.identifier.uri | https://hdl.handle.net/2027.42/63414 | |
dc.identifier.uri | http://www.ncbi.nlm.nih.gov/sites/entrez?cmd=retrieve&db=pubmed&list_uids=10779161&dopt=citation | en_US |
dc.description.abstract | We performed a clinical study of five patients with melanoma to evaluate the immunobiological effects of retrovirally transduced autologous tumor cells given as a vaccine to prime draining lymph nodes. Patients were inoculated with both wild-type (WT) and GM-CSF gene-transduced tumor cells in different extremities. Approximately 7 days later, vaccine-primed lymph nodes (VPLNs) were removed. There was an increased infiltration of dendritic cells (DCs) in the GM-CSF-secreting vaccine sites compared with the WT vaccine sites. This resulted in a greater number of cells harvested from the GM-CSF-VPLNs compared with the WT-VPLNs at a time when serum levels of GM-CSF were not detectable. Four of five patients proceeded to have the adoptive transfer of GM-CSF-VPLN cells secondarily activated and expanded ex vivo with anti-CD3 MAb and IL2. One patient had a durable complete remission of metastatic tumor. Utilizing cytokine (IFN-gamma, GM-CSF, IL-10) release assays, GM-CSF-VPLN T cells manifested diverse responses when exposed to tumor antigen in vitro. In two of two patients, GM-CSF-VPLN T cell responses were different from those of matched WTVPLN cells. This study documents measurable immunobiologic differences of GM-CSF-transduced tumor cells given as a vaccine compared with WT tumor cells. The complete tumor remission in one patient provides a rationale to pursue this approach further. | en_US |
dc.format.extent | 740518 bytes | |
dc.format.extent | 2489 bytes | |
dc.format.mimetype | application/pdf | |
dc.format.mimetype | text/plain | |
dc.publisher | Mary Ann Liebert, Inc., publishers | en_US |
dc.title | Immunogenetic Therapy of Human Melanoma Utilizing Autologous Tumor Cells Transduced to Secrete Granulocyte-Macrophage Colony-Stimulating Factor | en_US |
dc.type | Article | en_US |
dc.subject.hlbtoplevel | Health Sciences | en_US |
dc.description.peerreviewed | Peer Reviewed | en_US |
dc.identifier.pmid | 10779161 | en_US |
dc.description.bitstreamurl | http://deepblue.lib.umich.edu/bitstream/2027.42/63414/1/10430340050015455.pdf | |
dc.identifier.doi | doi:10.1089/10430340050015455 | en_US |
dc.identifier.source | Human Gene Therapy | en_US |
dc.identifier.source | Human Gene Therapy | en_US |
dc.owningcollname | Interdisciplinary and Peer-Reviewed |
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