Ultraviolet irradiation-induces epidermal growth factor receptor (EGFR) nuclear translocation in human keratinocytes
dc.contributor.author | Xu, Yiru | en_US |
dc.contributor.author | Shao, Yuan | en_US |
dc.contributor.author | Zhou, Jin | en_US |
dc.contributor.author | Voorhees, John J. | en_US |
dc.contributor.author | Fisher, Gary J. | en_US |
dc.date.accessioned | 2009-08-12T15:34:31Z | |
dc.date.available | 2010-10-05T18:27:29Z | en_US |
dc.date.issued | 2009-08-01 | en_US |
dc.identifier.citation | Xu, Yiru; Shao, Yuan; Zhou, Jin; Voorhees, John J.; Fisher, Gary J. (2009). "Ultraviolet irradiation-induces epidermal growth factor receptor (EGFR) nuclear translocation in human keratinocytes." Journal of Cellular Biochemistry 107(5): 873-880. <http://hdl.handle.net/2027.42/63537> | en_US |
dc.identifier.issn | 0730-2312 | en_US |
dc.identifier.issn | 1097-4644 | en_US |
dc.identifier.uri | https://hdl.handle.net/2027.42/63537 | |
dc.description.abstract | Epidermal growth factor receptor (EGFR) plays a critical role in mediating ultraviolet (UV) irradiation-induced signal transduction and gene expression in human keratinocytes. EGFR activation results from increased phosphorylation on specific tyrosine residues in the C-terminal intracellular domain. It has recently been reported that following growth factor stimulation EGFR translocates from the surface membrane to the nucleus, where it may directly regulate gene transcription. We have investigated the ability of UV irradiation to induce EGFR nuclear translocation in human primary and HaCaT keratinocytes. UV irradiation caused rapid nuclear translocation of EGFR. Significant accumulation of EGFR in the nucleus was observed within 15 min after UV irradiation exposure. Maximal translocation occurred at 30 min post-UV irradiation, and resulted in a 10-fold increase in EGFR in the nucleus, as determined by Western blot analysis of nuclear extracts and confirmed by immunofluorescence. Inhibition of nuclear export by Leptomycin B did not alter UV irradiation-induced nuclear accumulation. EGFR tyrosine kinase inhibitor (PD169540) reduced UV irradiation-induced EGFR nuclear translocation 50%. Mutation of either tyrosine 1148 or tyrosine 1173 reduced nuclear translocation 70%, while mutation of tyrosine 1068 was without effect. In addition, over-expression of receptor type protein tyrosine phosphatase-kappa (RPTP-Κ), which specifically dephosphorylates EGFR tyrosines, decreased UV irradiation-induced EGFR nuclear translocation in human keratinocytes. These data demonstrate that UV irradiation stimulates rapid EGFR nuclear translocation, which is dependent on phosphorylation of specific EGFR tyrosine residues. EGFR nuclear translocation may act in concert with conventional signaling pathways to mediate UV irradiation-induced responses in human keratinocytes. J. Cell. Biochem. 107: 873–880, 2009. © 2009 Wiley-Liss, Inc. | en_US |
dc.format.extent | 272609 bytes | |
dc.format.extent | 3118 bytes | |
dc.format.mimetype | application/pdf | |
dc.format.mimetype | text/plain | |
dc.publisher | Wiley Subscription Services, Inc., A Wiley Company | en_US |
dc.subject.other | Life and Medical Sciences | en_US |
dc.subject.other | Cell & Developmental Biology | en_US |
dc.title | Ultraviolet irradiation-induces epidermal growth factor receptor (EGFR) nuclear translocation in human keratinocytes | en_US |
dc.type | Article | en_US |
dc.rights.robots | IndexNoFollow | en_US |
dc.subject.hlbsecondlevel | Genetics | en_US |
dc.subject.hlbsecondlevel | Molecular, Cellular and Developmental Biology | en_US |
dc.subject.hlbtoplevel | Health Sciences | en_US |
dc.subject.hlbtoplevel | Science | en_US |
dc.description.peerreviewed | Peer Reviewed | en_US |
dc.contributor.affiliationum | Department of Dermatology, University of Michigan Medical School, Ann Arbor, Michigan 48109 | en_US |
dc.contributor.affiliationum | Department of Dermatology, University of Michigan Medical School, Ann Arbor, Michigan 48109 | en_US |
dc.contributor.affiliationum | Department of Dermatology, University of Michigan Medical School, Ann Arbor, Michigan 48109 | en_US |
dc.contributor.affiliationum | Department of Dermatology, University of Michigan Medical School, Ann Arbor, Michigan 48109 | en_US |
dc.contributor.affiliationum | Department of Dermatology, University of Michigan Medical School, Ann Arbor, Michigan 48109 ; Department of Dermatology, University of Michigan Medical School, Medical Science I, Room 6447, 1150 W. Medical Center Drive, Ann Arbor, MI 48109-5609. | en_US |
dc.identifier.pmid | 19415674 | en_US |
dc.description.bitstreamurl | http://deepblue.lib.umich.edu/bitstream/2027.42/63537/1/22195_ftp.pdf | |
dc.identifier.doi | 10.1002/jcb.22195 | en_US |
dc.identifier.source | Journal of Cellular Biochemistry | en_US |
dc.owningcollname | Interdisciplinary and Peer-Reviewed |
Files in this item
Remediation of Harmful Language
The University of Michigan Library aims to describe library materials in a way that respects the people and communities who create, use, and are represented in our collections. Report harmful or offensive language in catalog records, finding aids, or elsewhere in our collections anonymously through our metadata feedback form. More information at Remediation of Harmful Language.
Accessibility
If you are unable to use this file in its current format, please select the Contact Us link and we can modify it to make it more accessible to you.