The Role of PTIP in Maintaining Embryonic Stem Cell Pluripotency
dc.contributor.author | Kim, Doyeob | en_US |
dc.contributor.author | Patel, Sanjeevkumar R. | en_US |
dc.contributor.author | Xiao, Hong | en_US |
dc.contributor.author | Dressler, Gregory R. | en_US |
dc.date.accessioned | 2009-08-12T15:35:19Z | |
dc.date.available | 2010-09-01T19:24:06Z | en_US |
dc.date.issued | 2009-07 | en_US |
dc.identifier.citation | Kim, Doyeob; Patel, Sanjeevkumar R.; Xiao, Hong; Dressler, Gregory R. (2009). "The Role of PTIP in Maintaining Embryonic Stem Cell Pluripotency Author contributions: D.K.: conception and design, collection and assembly of data, data analysis; S.P.: collection and assembly of data, data analysis; H.X.: collection and assembly of data; G.D.: conception and design, collection and assembly of data, data analysis, manuscript writing, financial support. First published online in STEM CELLS EXPRESS April 2, 2009. ." Stem Cells 27(7): 1516-1523. <http://hdl.handle.net/2027.42/63546> | en_US |
dc.identifier.issn | 1066-5099 | en_US |
dc.identifier.issn | 1549-4918 | en_US |
dc.identifier.uri | https://hdl.handle.net/2027.42/63546 | |
dc.description.abstract | Pax transactivation domain-interacting protein (PTIP) is a ubiquitously expressed, nuclear protein that is part of a histone H3K4 methyltransferase complex and is essential for embryonic development. Methylation of H3K4 is an epigenetic mark found on many critical developmental regulatory genes in embryonic stem (ES) cells and, together with H3K27 methylation, constitutes a bivalent epigenetic signature. To address the function of PTIP in ES cells, we generated ES cell lines from a floxed ptip allele and deleted PTIP function with Cre recombinase. The ptip −/− ES cell lines exhibited a high degree of spontaneous differentiation to trophectoderm and a loss of pluripotency. Reduced levels of Oct4 expression and H3K4 methylation were observed. Upon differentiation, ptip −/− embryoid bodies showed reduced levels of marker gene expression for all three primary germ layers. These results suggest that the maintenance of H3K4 methylation is essential and requires PTIP function during the in vitro propagation of pluripotent ES cells. STEM CELLS 2009;27:1516–1523 | en_US |
dc.format.extent | 724568 bytes | |
dc.format.extent | 3118 bytes | |
dc.format.mimetype | application/pdf | |
dc.format.mimetype | text/plain | |
dc.publisher | Wiley Subscription Services, Inc., A Wiley Company | en_US |
dc.subject.other | Life and Medical Sciences | en_US |
dc.subject.other | Cell and Molecular Biology | en_US |
dc.title | The Role of PTIP in Maintaining Embryonic Stem Cell Pluripotency | en_US |
dc.type | Article | en_US |
dc.rights.robots | IndexNoFollow | en_US |
dc.subject.hlbsecondlevel | Molecular, Cellular and Developmental Biology | en_US |
dc.subject.hlbtoplevel | Health Sciences | en_US |
dc.description.peerreviewed | Peer Reviewed | en_US |
dc.contributor.affiliationum | Department of Pathology, University of Michigan, Ann Arbor, Michigan, USA | en_US |
dc.contributor.affiliationum | Department of Pathology, University of Michigan, Ann Arbor, Michigan, USA | en_US |
dc.contributor.affiliationum | Department of Pathology, University of Michigan, Ann Arbor, Michigan, USA | en_US |
dc.contributor.affiliationum | Department of Pathology, University of Michigan, Ann Arbor, Michigan, USA ; Telephone: 734-764-6490; Fax: 734-763-2162 ; Department of Pathology, University of Michigan, 2049 BSRB, 109 Zina Pitcher Place, Ann Arbor, Michigan 48109, USA | en_US |
dc.identifier.pmid | 19544464 | en_US |
dc.description.bitstreamurl | http://deepblue.lib.umich.edu/bitstream/2027.42/63546/1/79_ftp.pdf | |
dc.identifier.doi | 10.1002/stem.79 | en_US |
dc.identifier.source | Stem Cells | en_US |
dc.owningcollname | Interdisciplinary and Peer-Reviewed |
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