Differentiation of Neural Progenitor Cells in a Microfluidic Chip-Generated Cytokine Gradient
dc.contributor.author | Park, Joong Yull | en_US |
dc.contributor.author | Kim, Suel-Kee | en_US |
dc.contributor.author | Woo, Dong-Hun | en_US |
dc.contributor.author | Lee, Eun-Joong | en_US |
dc.contributor.author | Kim, Jong-Hoon | en_US |
dc.contributor.author | Lee, Sang-Hoon | en_US |
dc.date.accessioned | 2009-11-30T16:44:02Z | |
dc.date.available | 2010-03-01T21:10:28Z | en_US |
dc.date.issued | 2009-11 | en_US |
dc.identifier.citation | Park, Joong Yull; Kim, Suel-Kee; Woo, Dong-Hun; Lee, Eun-Joong; Kim, Jong-Hoon; Lee, Sang-Hoon (2009). "Differentiation of Neural Progenitor Cells in a Microfluidic Chip-Generated Cytokine Gradient Author contributions: J.Y.P. and S.-K.K.: conception and design, data analysis and interpretation, manuscript writing; D.-H.W. and E.-J.L.: collection and/or assembly of data; J.-H.K. and S.-H.L.: financial support, manuscript writing, final approval of manuscript. Disclosure of potential conflicts of interest is found at the end of this article. First published online in STEM CELLS EXPRESS 2009. ." Stem Cells 27(11): 2646-2654. <http://hdl.handle.net/2027.42/64440> | en_US |
dc.identifier.issn | 1066-5099 | en_US |
dc.identifier.issn | 1549-4918 | en_US |
dc.identifier.uri | https://hdl.handle.net/2027.42/64440 | |
dc.identifier.uri | http://www.ncbi.nlm.nih.gov/sites/entrez?cmd=retrieve&db=pubmed&list_uids=19711444&dopt=citation | en_US |
dc.description.abstract | In early embryonic development, spatial gradients of diffusible signaling molecules play important roles in controlling differentiation of cell types or arrays in diverse tissues. Thus, the concentration of exogenous cytokines or growth factors at any given time is crucial to the formation of an enriched population of a desired cell type from primitive stem cells in vitro. Microfluidic technology has proven very useful in the creation of cell-friendly microenvironments. Such techniques are, however, currently limited to a few cell types. Improved versatility is required if these systems are to become practically applicable to stem cells showing various plasticity ranges. Here, we built a microfluidic platform in which cells can be exposed to stable concentration gradients of various signaling molecules for more than a week with only minimal handling and no external power source. To maintain stability of the gradient concentration, the osmotic pumping performance was optimized by balancing the capillary action and hydraulic pressure in the inlet reagent reservoirs. We cultured an enriched population of neural progenitors derived from human embryonic stem cells in our microfluidic chamber for 8 days under continuous cytokine gradients (sonic hedgehog, fibroblast growth factor 8, and bone morphogenetic protein 4). Neural progenitors successfully differentiated into neurons, generating a complex neural network. The average numbers of both neuronal cell body clusters and neurite bundles were directly proportional to sonic hedgehog concentrations in the gradient chip. The system was shown to be useful for both basic and translational research, with straightforward mechanisms and operational schemes. S TEM C ELLS 2009;27:2646–2654 | en_US |
dc.format.extent | 513444 bytes | |
dc.format.extent | 3118 bytes | |
dc.format.mimetype | application/pdf | |
dc.format.mimetype | text/plain | |
dc.publisher | Wiley Subscription Services, Inc., A Wiley Company | en_US |
dc.subject.other | Life and Medical Sciences | en_US |
dc.subject.other | Cell and Molecular Biology | en_US |
dc.title | Differentiation of Neural Progenitor Cells in a Microfluidic Chip-Generated Cytokine Gradient | en_US |
dc.type | Article | en_US |
dc.rights.robots | IndexNoFollow | en_US |
dc.subject.hlbsecondlevel | Molecular, Cellular and Developmental Biology | en_US |
dc.subject.hlbtoplevel | Health Sciences | en_US |
dc.description.peerreviewed | Peer Reviewed | en_US |
dc.contributor.affiliationum | Department of Biomedical Engineering, College of Health Science, Korea University, Seoul, Republic of Korea ; Department of Biomedical Engineering, College of Engineering, University of Michigan, Ann Arbor, Michigan, USA | en_US |
dc.contributor.affiliationother | Division of Biotechnology, College of Life Sciences and Biotechnology, Korea University, Seoul, Republic of Korea | en_US |
dc.contributor.affiliationother | Division of Biotechnology, College of Life Sciences and Biotechnology, Korea University, Seoul, Republic of Korea | en_US |
dc.contributor.affiliationother | Department of Biomedical Engineering, College of Health Science, Korea University, Seoul, Republic of Korea | en_US |
dc.contributor.affiliationother | Division of Biotechnology, College of Life Sciences and Biotechnology, Korea University, Seoul, Republic of Korea ; Telephone: +82-2-3290-3007; Fax: +82-2-3290-3507 ; Division of Biotechnology, College of Life Sciences and Biotechnology, Korea University, Anam-dong, Seongbuk-gu, Seoul 136-705, Republic of Korea | en_US |
dc.contributor.affiliationother | Department of Biomedical Engineering, College of Health Science, Korea University, Seoul, Republic of Korea ; Telephone: +82-2-940-2881; Fax: +82-2-921-6818 ; Department of Biomedical Engineering, College of Health Science, Korea University, Jeongneung-dong, Seongbuk-gu, Seoul 136-703, Republic of Korea | en_US |
dc.identifier.pmid | 19711444 | en_US |
dc.description.bitstreamurl | http://deepblue.lib.umich.edu/bitstream/2027.42/64440/1/202_ftp.pdf | |
dc.identifier.doi | 10.1002/stem.202 | en_US |
dc.identifier.source | Stem Cells | en_US |
dc.owningcollname | Interdisciplinary and Peer-Reviewed |
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