PROTEIN SYNTHESIS IN NEURONAL PERIKARYA ISOLATED FROM CEREBRAL CORTEX OF THE IMMATURE RAT 1
dc.contributor.author | Kohl, Herbert H. | en_US |
dc.contributor.author | Sellinger, Otto Z. | en_US |
dc.date.accessioned | 2010-04-01T14:41:30Z | |
dc.date.available | 2010-04-01T14:41:30Z | |
dc.date.issued | 1972-03 | en_US |
dc.identifier.citation | Kohl, H. H.; Sellinger, O. Z. (1972). "PROTEIN SYNTHESIS IN NEURONAL PERIKARYA ISOLATED FROM CEREBRAL CORTEX OF THE IMMATURE RAT 1 ." Journal of Neurochemistry 19(3): 699-711. <http://hdl.handle.net/2027.42/65135> | en_US |
dc.identifier.issn | 0022-3042 | en_US |
dc.identifier.issn | 1471-4159 | en_US |
dc.identifier.uri | https://hdl.handle.net/2027.42/65135 | |
dc.identifier.uri | http://www.ncbi.nlm.nih.gov/sites/entrez?cmd=retrieve&db=pubmed&list_uids=5030982&dopt=citation | en_US |
dc.description.abstract | Homogenates of neuronal perikarya isolated from the cerebral cortex of the 8-day-old rat were incubated with [ 3 H]leucine, and the characteristics of the protein synthetic process were studied. Incorporation of leucine into protein was linear up to 90 min, proceeded optimally at pH 7.6 and was stimulated by K + and NH 4 + , unaffected by Li + and inhibited by Na + . Puromycin, cycloheximide, RNAse, sulphhydryl blocking agents and phospholipase A exerted a pronounced inhibition, whereas chloramphenicol and phospholipase C had no effect. About 42 per cent of the total radioactive protein formed in the optimally fortified in uitro system was recovered in non-sedimentable form. Incorporation into the subcellular fractions of the neuronal perikarya increased steadily with increasing time of incubation. The microsomal fraction acquired the highest specific radioactivity (d.p.m./mg of protein), followed by the mitochondrial and the nuclear + cell debris fractions. The high-speed soluble fraction exhibited the lowest specific radioactivity. Although the addition of L-methionine to a suitably fortified incubation medium inhibited neuronal protein synthesis by about 80 per cent, the addition of D-methionhe, Α-methyl-DL-methionine or L-tryptophan was relatively ineffective by comparison. | en_US |
dc.format.extent | 1004578 bytes | |
dc.format.extent | 3110 bytes | |
dc.format.mimetype | application/pdf | |
dc.format.mimetype | text/plain | |
dc.publisher | Blackwell Publishing Ltd | en_US |
dc.rights | 1972 International Society for Neurochemistry Ltd. | en_US |
dc.title | PROTEIN SYNTHESIS IN NEURONAL PERIKARYA ISOLATED FROM CEREBRAL CORTEX OF THE IMMATURE RAT 1 | en_US |
dc.type | Article | en_US |
dc.rights.robots | IndexNoFollow | en_US |
dc.subject.hlbsecondlevel | Neurosciences | en_US |
dc.subject.hlbtoplevel | Health Sciences | en_US |
dc.description.peerreviewed | Peer Reviewed | en_US |
dc.contributor.affiliationum | Mental Health Research Institute, The University of Michigan Medical Centre, Ann Arbor, Michigan 48104. | en_US |
dc.identifier.pmid | 5030982 | en_US |
dc.description.bitstreamurl | http://deepblue.lib.umich.edu/bitstream/2027.42/65135/1/j.1471-4159.1972.tb01385.x.pdf | |
dc.identifier.doi | 10.1111/j.1471-4159.1972.tb01385.x | en_US |
dc.identifier.source | Journal of Neurochemistry | en_US |
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