Kanamycin alters cytoplasmic and nuclear phosphoinositide signaling in the organ of Corti in vivo
dc.contributor.author | Jiang, Hongyan | en_US |
dc.contributor.author | Sha, Su-Hua | en_US |
dc.contributor.author | Schacht, Jochen | en_US |
dc.date.accessioned | 2010-04-01T14:43:21Z | |
dc.date.available | 2010-04-01T14:43:21Z | |
dc.date.issued | 2006-10 | en_US |
dc.identifier.citation | Jiang, Hongyan; Sha, Su-Hua; Schacht, Jochen (2006). "Kanamycin alters cytoplasmic and nuclear phosphoinositide signaling in the organ of Corti in vivo ." Journal of Neurochemistry 99(1): 269-276. <http://hdl.handle.net/2027.42/65167> | en_US |
dc.identifier.issn | 0022-3042 | en_US |
dc.identifier.issn | 1471-4159 | en_US |
dc.identifier.uri | https://hdl.handle.net/2027.42/65167 | |
dc.identifier.uri | http://www.ncbi.nlm.nih.gov/sites/entrez?cmd=retrieve&db=pubmed&list_uids=16903869&dopt=citation | en_US |
dc.description.abstract | Aminoglycoside antibiotics strongly bind to phosphoinositides and affect their membrane distribution and metabolism. Kanamycin treatment also disrupts Rac/Rho signaling pathways to the actin cytoskeleton in the mouse inner ear in vivo . Here, we investigate the influence of kanamycin on phosphoinositide signaling in sensory cells (hair cells) of the mouse cochlea. Immunoreactivity to phosphatidylinositol-3,4,5-trisphosphate (PIP 3 ) decreased in the organ of Corti, especially in outer hair cells, after 3–7 days of drug treatment, whereas imunoreactivity to phosphatidylinositol-4,5-bisphosphate (PIP 2 ) increased. Immunoreactivity to PIP 2 was present at the apical poles of outer hair cells, but appeared in their nuclei only after drug treatment. Furthermore, nuclear PIP 2 formed a complex with histone H3 and attenuated its acetylation in outer hair cells. In agreement with reduced PIP 3 signaling, phosphorylated Akt decreased in both the cytoplasm and nuclei of outer hair cells after kanamycin treatment. This study suggests that kanamycin disturbs the balance between PIP 2 and PIP 3 , modifies gene transcription via histone acetylation and diminishes the PI3K/Akt survival pathway. These actions may contribute to the death of outer hair cells, which is a consequence of chronic kanamycin treatment. | en_US |
dc.format.extent | 1007862 bytes | |
dc.format.extent | 3110 bytes | |
dc.format.mimetype | application/pdf | |
dc.format.mimetype | text/plain | |
dc.publisher | Blackwell Publishing Ltd | en_US |
dc.rights | 2006 The Authors Journal Compilation 2006 International Society for Neurochemistry | en_US |
dc.subject.other | Akt | en_US |
dc.subject.other | Histone | en_US |
dc.subject.other | Outer Hair Cells | en_US |
dc.subject.other | Phosphatidylinositol-4, 5-bisphosphate | en_US |
dc.subject.other | Phosphatidylinositol-3, 4, 5-trisphosphate | en_US |
dc.subject.other | Signaling Pathways | en_US |
dc.title | Kanamycin alters cytoplasmic and nuclear phosphoinositide signaling in the organ of Corti in vivo | en_US |
dc.type | Article | en_US |
dc.rights.robots | IndexNoFollow | en_US |
dc.subject.hlbsecondlevel | Neurosciences | en_US |
dc.subject.hlbtoplevel | Health Sciences | en_US |
dc.description.peerreviewed | Peer Reviewed | en_US |
dc.contributor.affiliationum | * Kresge Hearing Research Institute, Department of Otolaryngology, University of Michigan, Ann Arbor, Michigan, USA | en_US |
dc.contributor.affiliationother | † Otorhinolaryngological Hospital of First Affiliated Hospital of Sun Yat-Sen University, Guangzhou, China | en_US |
dc.identifier.pmid | 16903869 | en_US |
dc.description.bitstreamurl | http://deepblue.lib.umich.edu/bitstream/2027.42/65167/1/j.1471-4159.2006.04117.x.pdf | |
dc.identifier.doi | 10.1111/j.1471-4159.2006.04117.x | en_US |
dc.identifier.source | Journal of Neurochemistry | en_US |
dc.identifier.citedreference | Arbuzova A., Martushova K., Hangyas-Mihalyne G., Morris A. J., Ozaki S., Prestwich G. D. and McLaughlin S. ( 2000 ) Fluorescently labeled neomycin as a probe of phosphatidylinositol-4, 5-bisphosphate in membranes. Biochim. Biophys. Acta 1464, 35 – 48. | en_US |
dc.identifier.citedreference | Balla T., Bondeva T. and Varnai P. ( 2000 ) How accurately can we image inositol lipids in living cells? Trends Pharmacol. Sci. 21, 238 – 241. | en_US |
dc.identifier.citedreference | Berthiaume M., Boufaied N., Moisan A. and Gaudreau L. ( 2006 ) High levels of oxidative stress globally inhibit gene transcription and histone acetylation. DNA Cell Biol. 25, 124 – 134. | en_US |
dc.identifier.citedreference | Boronenkov I. V., Loijens J. C., Umeda M. and Anderson R. A. ( 1998 ) Phosphoinositide signaling pathways in nuclei are associated with nuclear speckles containing pre-mRNA processing factors. Mol. Biol. Cell 9, 3547 – 3560. | en_US |
dc.identifier.citedreference | Cooper J. A. and Schafer D. A. ( 2000 ) Control of actin assembly and disassembly at filament ends. Curr. Opin. Cell Biol. 12, 97 – 103. | en_US |
dc.identifier.citedreference | De Matteis M. A., Di Campli A. and Godi A. ( 2005 ) The role of the phosphoinositides at the Golgi complex. Biochim. Biophys. Acta 1744, 396 – 405. | en_US |
dc.identifier.citedreference | Enomoto R., Yoshida Y., Komai T., Sugahara C., Yasuoka Y. and Lee E. ( 2003 ) Involvement of the change in chromatin structure in thymocyte apoptosis induced by phosphorylation of histones. Ann. NY Acad. Sci. 1010, 218 – 220. | en_US |
dc.identifier.citedreference | Forge A. and Schacht J. ( 2000 ) Aminoglycoside antibiotics. Audiol. Neurotol. 5, 3 – 22. | en_US |
dc.identifier.citedreference | Fukami K., Matsuoka K., Nakanishi O., Yamakawa A., Kawai S. and Takenawa T. ( 1988 ) Antibody to phosphatidylinositol 4,5-bis-phosphate inhibits oncogene-induced mitogenesis. Proc. Natl Acad. Sci. USA 85, 9057 – 9061. | en_US |
dc.identifier.citedreference | Gonzales M. L. and Anderson R. A. ( 2006 ) Nuclear phosphoinositide kinases and inositol lipids. J. Cell. Biochem. 97, 252 – 260. | en_US |
dc.identifier.citedreference | Hirono M., Denis C. S., Richardson G. P. and Gillespie P. G. ( 2004 ) Hair cells require phosphatidylinositol 4,5-bisphosphate for mechanical transduction and adaptation. Neuron 44, 309 – 320. | en_US |
dc.identifier.citedreference | Holz R. W., Hlubek M. D., Sorensen S. D., Fisher S. K., Balla T., Ozaki S., Prestwich G. D., Stuenkel E. L. and Bittner M. A. ( 2000 ) A pleckstrin homology domain specific for phosphatidylinositol 4,5-bisphosphate (PtdIns-4,5-P-2) and fused to green fluorescent protein identifies plasma membrane PtdIns-4,5-P-2 as being important in exocytosis. J. Biol. Chem. 275, 17 878 – 17 885. | en_US |
dc.identifier.citedreference | Irvine R. F. ( 2003 ) Nuclear lipid signaling. Nat. Rev. Mol. Cell Biol. 4, 1 – 12. | en_US |
dc.identifier.citedreference | Janmey P. A. and Stossel T. P. ( 1989 ) Gelsolin–polyphosphoinositide interaction. Full expression of gelsolin-inhibiting function by polyphosphoinositides in vesicular form and inactivation by dilution, aggregation, or masking of the inositol head group. J. Biol. Chem. 264, 4825 – 4831. | en_US |
dc.identifier.citedreference | Jiang H., Sha S. H. and Schacht J. ( 2005 ) NF-βB pathway protects cochlear hair cells from aminoglycoside-induced ototoxicity. J. Neurosci. Res. 79, 644 – 651. | en_US |
dc.identifier.citedreference | Jiang H., Sha S. H., Forge A. and Schacht J. ( 2006a ) Caspase-independent pathways of hair cell death induced by kanamycin in vivo. Cell Death Differ. 13, 20 – 30. | en_US |
dc.identifier.citedreference | Jiang H., Sha S. H. and Schacht J. ( 2006b ) Rac/Rho pathway regulates actin depolymerization induced by aminoglycoside antibiotics. J. Neurosci. 83, 1544 – 1551. DOI: 10.1002/jnr.20833. | en_US |
dc.identifier.citedreference | Lesniak W., Pecoraro V. L. and Schacht J. ( 2005 ) Ternary complexes of gentamicin with iron and lipid catalyze formation of reactive oxygen species. Chem. Res. Toxicol. 18, 357 – 364. | en_US |
dc.identifier.citedreference | Lodhi S., Weiner N. D. and Schacht J. ( 1979 ) Interactions of neomycin with monomolecular films of polyphosphoinositides and other lipids. Biochim. Biophys. Acta 557, 1 – 8. | en_US |
dc.identifier.citedreference | Martelli A. M., Follo M. Y., Evangelisti C., FalÀ F., Fiume R., Billi A. M. and Cocco L. ( 2005 ) Nuclear inositol lipid metabolism: more than just second messenger generation?. J. Cell. Biochem. 96, 285 – 292. | en_US |
dc.identifier.citedreference | Montcouquiol M. and Corwin J. T. ( 2001 ) Intracellular signals that control cell proliferation in mammalian balance epithelia: key roles for phosphatidylinositol-3 kinase, mammalian target of rapamycin, and S6 kinases in preference to calcium, protein kinase C, and mitogen-activated protein kinase. J. Neurosci. 21, 570 – 580. | en_US |
dc.identifier.citedreference | Nagy I., Monge A., Albinger-Hegyi A., Schmid S. and Bodmer D. ( 2005 ) NF-kappaB is required for survival of immature auditory hair cells in vitro. JARO 6, 260 – 268. | en_US |
dc.identifier.citedreference | Neri L. M., Borgatti P., Capitani S. and Martelli A. M. ( 2002 ) The nuclear phosphoinositide 3-kinase/AKT pathway: a new second messenger system. Biochim. Biophys. Acta 1584, 73 – 80. | en_US |
dc.identifier.citedreference | Nicholson K. M. and Anderson N. G. ( 2002 ) The protein kinaseB/Akt signaling pathway in human malignancy. Cell. Signal. 14, 381 – 395. | en_US |
dc.identifier.citedreference | Orsulakova A., Stockhorst E. and Schacht J. ( 1976 ) Effect of neomycin on phosphoinositide labelling and calcium binding in guinea-pig inner ear tissues in vivo and in vitro. J. Neurochem. 26, 285 – 290. | en_US |
dc.identifier.citedreference | Osborne S. L., Thomas C. L., Gschmeissner S. and Schiavo G. ( 2001 ) Nuclear PtdIns (4,5),P2 assembles in a mitotically regulated particle involved in pre-mRNA splicing. J. Cell Sci. 114, 2501 – 2511. | en_US |
dc.identifier.citedreference | Parker P. J. ( 2004 ) The ubiquitous phosphoinositides. Biochem. Soc. Trans. 32, 893 – 898. | en_US |
dc.identifier.citedreference | Prigent C. and Dimitrov S. ( 2003 ) Phosphorylation of serine 10 in histone H3, what for?. J. Cell Sci. 116, 3677 – 3685. | en_US |
dc.identifier.citedreference | Priuska E. and Schacht J. ( 1995 ) Formation of free radicals by gentamicin and iron and evidence for an iron/gentamicin complex. Biochem. Pharmacol. 50, 1749 – 1752. | en_US |
dc.identifier.citedreference | Schacht J. ( 1976 ) Inhibition by neomycin of polyphosphoinositide turnover in subcellular fractions of guinea-pig cerebral cortex in vitro. J. Neurochem. 27, 1119 – 1124. | en_US |
dc.identifier.citedreference | Schacht J. ( 1979 ) Isolation of an aminoglycoside receptor from guinea pig inner ear tissues and kidney. Arch. Oto-Rhino-Laryngol. 224, 129 – 134. | en_US |
dc.identifier.citedreference | Song G., Ouyang G. and Bao S. ( 2005 ) The activation of Akt/PKB signaling pathway and cell survival. J. Cell. Mol. Med. 9, 59 – 71. | en_US |
dc.identifier.citedreference | Takenawa T. and Miki H. ( 2001 ) WASP and WAVE family proteins: key molecules for rapid rearrangement of cortical actin filaments and cell movement. J. Cell Sci. 114, 1801 – 1809. | en_US |
dc.identifier.citedreference | Thomas C. L., Steel J., Prestwich G. D. and Schiavo G. ( 1999 ) Generation of phosphatidylinositol-specific antibodies and their characterization. Biochem. Soc. Trans. 27, 648 – 652. | en_US |
dc.identifier.citedreference | Varnai P. and Balla T. ( 1998 ) Visualization of phosphoinositides that bind pleckstrin homology domains: calcium- and agonist-induced dynamic changes and relationship to myo-( 3 H) inositol labeled phosphoinositide pools. J. Cell. Biol. 143, 501 – 510. | en_US |
dc.identifier.citedreference | Verdone L., Caserta M. and Di Mauro E. ( 2005 ) Role of histone acetylation in the control of gene expression. Biochem. Cell Biol. 83, 344 – 353. | en_US |
dc.identifier.citedreference | Williams S. E., Zenner H. P. and Schacht J. ( 1987 ) Three molecular steps of aminoglycoside ototoxicity demonstrated in outer hair cells. Hear. Res. 30, 11 – 18. | en_US |
dc.identifier.citedreference | Woodgett J. R. ( 2005 ) Recent advances in the protein kinase B signaling pathway. Curr. Opin. Cell Biol. 17, 150 – 157. | en_US |
dc.identifier.citedreference | Wu W. J., Sha S. H., McLaren J. D., Kawamoto K., Raphael Y. and Schacht J. ( 2001 ) Aminoglycoside ototoxicity in adult CBA, C57BL and BALB mice and the Sprague-Dawley rat. Hear. Res. 158, 165 – 178. | en_US |
dc.identifier.citedreference | Yu H., Fukami K., Watanabe Y., Ozaki C. and Takenawa T. ( 1998 ) Phosphatidylinositol 4,5-bisphosphate reverses the inhibition of RNA transcription caused by histone H1. Eur. J. Biochem. 251, 281 – 287. | en_US |
dc.owningcollname | Interdisciplinary and Peer-Reviewed |
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