Purification and Characterization of p68/70, Regeneration-Associated Proteins from Goldfish Brain
dc.contributor.author | Leski, Michael L. | en_US |
dc.contributor.author | Agranoff, Bernard W. | en_US |
dc.date.accessioned | 2010-04-01T14:45:33Z | |
dc.date.available | 2010-04-01T14:45:33Z | |
dc.date.issued | 1994-03 | en_US |
dc.identifier.citation | Leski, Michael L.; Agranoff, Bernard W. (1994). "Purification and Characterization of p68/70, Regeneration-Associated Proteins from Goldfish Brain." Journal of Neurochemistry 62(3): 1182-1191. <http://hdl.handle.net/2027.42/65206> | en_US |
dc.identifier.issn | 0022-3042 | en_US |
dc.identifier.issn | 1471-4159 | en_US |
dc.identifier.uri | https://hdl.handle.net/2027.42/65206 | |
dc.identifier.uri | http://www.ncbi.nlm.nih.gov/sites/entrez?cmd=retrieve&db=pubmed&list_uids=8113803&dopt=citation | en_US |
dc.description.abstract | Two acidic proteins (p68/70) previously shown to be associated with regeneration of the goldfish optic nerve were purified 887-fold from brain homogenates of Carassius auratus. Purification to homogeneity was achieved by sequential chromatography of a 100,000 g brain supernatant fraction on DEAE-Sephacel, Cu 2+ -charged iminodiacetic acid agarose, and gel filtration. The Stokes radius of the doublet was determined to be 5.8 nm, and the sedimentation coefficient calculated to be 5 2. From these values a molecular mass of 128 kDa and a frictional coefficient ratio of 1.6 were calculated. Chromatofocusing on a high-resolution DEAE column resolved the protein doublet into three dimeric species of p68, p68/70, and p70. These results indicate that the proteins are highly elongated and associate as homodimers or as a hetero-dimer. Subcellular localization and membrane extraction experiments indicated p68/70 to be a component of the plasma membrane associated primarily through hydro-phobic interactions. p68/70 demonstrated biphasic behavior in phase partition experiments using Triton 114. Analysis of hydrolytic products indicated p68/70 to be a glyco-protein, containing 11% carbohydrate. | en_US |
dc.format.extent | 1263334 bytes | |
dc.format.extent | 3110 bytes | |
dc.format.mimetype | application/pdf | |
dc.format.mimetype | text/plain | |
dc.publisher | Blackwell Publishing Ltd | en_US |
dc.rights | 1994 International Society for Neurochemistry | en_US |
dc.subject.other | Goldfish | en_US |
dc.subject.other | Glycoprotein | en_US |
dc.subject.other | Plasma Membrane | en_US |
dc.subject.other | Regeneration | en_US |
dc.subject.other | Optic Nerve | en_US |
dc.title | Purification and Characterization of p68/70, Regeneration-Associated Proteins from Goldfish Brain | en_US |
dc.type | Article | en_US |
dc.rights.robots | IndexNoFollow | en_US |
dc.subject.hlbsecondlevel | Neurosciences | en_US |
dc.subject.hlbtoplevel | Health Sciences | en_US |
dc.description.peerreviewed | Peer Reviewed | en_US |
dc.contributor.affiliationum | Department of Biological Chemistry and Mental Health Research Institute, University of Michigan, Ann Arbor, Michigan, U.S.A. | en_US |
dc.identifier.pmid | 8113803 | en_US |
dc.description.bitstreamurl | http://deepblue.lib.umich.edu/bitstream/2027.42/65206/1/j.1471-4159.1994.62031182.x.pdf | |
dc.identifier.doi | 10.1046/j.1471-4159.1994.62031182.x | en_US |
dc.identifier.source | Journal of Neurochemistry | en_US |
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dc.owningcollname | Interdisciplinary and Peer-Reviewed |
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