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Characterization of Glutamate Uptake into Synaptic Vesicles
dc.contributor.authorNaito, Shigetakaen_US
dc.contributor.authorUeda, Tetsufumien_US
dc.date.accessioned2010-04-01T14:47:29Z
dc.date.available2010-04-01T14:47:29Z
dc.date.issued1985-01en_US
dc.identifier.citationNaito, Shigetaka; Ueda, Tetsufumi (1985). "Characterization of Glutamate Uptake into Synaptic Vesicles." Journal of Neurochemistry 44(1): 99-109. <http://hdl.handle.net/2027.42/65240>en_US
dc.identifier.issn0022-3042en_US
dc.identifier.issn1471-4159en_US
dc.identifier.urihttps://hdl.handle.net/2027.42/65240
dc.identifier.urihttp://www.ncbi.nlm.nih.gov/sites/entrez?cmd=retrieve&db=pubmed&list_uids=2856886&dopt=citationen_US
dc.description.abstractRecent evidence indicates that L-glutamate is taken up into synaptic vesicles in an ATP-dependent manner, supporting the notion that synaptic vesicles may be involved in glutamate synaptic transmission. In this study, we further characterized the ATP-dependent vesicular uptake of glutamate. Evidence is provided that a Mg-ATPase, not Ca-ATPase, is responsible for the ATP hydrolysis coupled to the glutamate uptake. The ATP-dependent glutamate uptake was inhibited by agents known to dissipate the electrochemical proton gradient across the membrane of chromaffin granules. Hence, it is suggested that the vesicular uptake of glutamate is driven by electrochemical proton gradients generated by the Mg-ATPase. Of particular interest is the finding that the ATP-dependent glutamate uptake is markedly stimulated by chloride over a physiologically relevant, millimolar concentration range, suggesting an important role of intranerve terminal chloride in the accumulation of glutamate in synaptic vesicles. The vesicular glutamate translocator is highly specific for L-glutamate, and failed to interact with aspartate, its related agents, and most of the glutamate analogs tested. It is proposed that this vesicular translocator plays a crucial role in determining the fate of glutamate as a neurotransmitter.en_US
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dc.publisherBlackwell Publishing Ltden_US
dc.rights1985 International Society for Neurochemistryen_US
dc.subject.otherGlutamateen_US
dc.subject.otherSynaptic Vesicleen_US
dc.subject.otherUptakeen_US
dc.subject.otherMg-ATPaseen_US
dc.subject.otherExcitatory Neurotransmitteren_US
dc.titleCharacterization of Glutamate Uptake into Synaptic Vesiclesen_US
dc.typeArticleen_US
dc.rights.robotsIndexNoFollowen_US
dc.subject.hlbsecondlevelNeurosciencesen_US
dc.subject.hlbtoplevelHealth Sciencesen_US
dc.description.peerreviewedPeer Revieweden_US
dc.contributor.affiliationum* Mental Health Research Institute, The University of Michigan, Ann Arbor, Michigan, U.S.A.en_US
dc.contributor.affiliationumDepartments of Pharmacology and Psychiatry, The University of Michigan, Ann Arbor, Michigan, U.S.A.en_US
dc.identifier.pmid2856886en_US
dc.description.bitstreamurlhttp://deepblue.lib.umich.edu/bitstream/2027.42/65240/1/j.1471-4159.1985.tb07118.x.pdf
dc.identifier.doi10.1111/j.1471-4159.1985.tb07118.xen_US
dc.identifier.sourceJournal of Neurochemistryen_US
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dc.owningcollnameInterdisciplinary and Peer-Reviewed


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