Blood-Brain Barrier Transport of 1-Aminocyclohexanecarboxylic Acid, a Nonmetabolizable Amino Acid for In Vivo Studies of Brain Transport
dc.contributor.author | Aoyagi, Masaki | en_US |
dc.contributor.author | Agranoff, Bernard W. | en_US |
dc.contributor.author | Washburn, Lee C. | en_US |
dc.contributor.author | Smith, Quentin R. | en_US |
dc.date.accessioned | 2010-04-01T14:50:02Z | |
dc.date.available | 2010-04-01T14:50:02Z | |
dc.date.issued | 1988-04 | en_US |
dc.identifier.citation | Aoyagi, Masaki; Agranoff, Bernard W.; Washburn, Lee C.; Smith, Quentin R. (1988). "Blood-Brain Barrier Transport of 1-Aminocyclohexanecarboxylic Acid, a Nonmetabolizable Amino Acid for In Vivo Studies of Brain Transport." Journal of Neurochemistry 50(4): 1220-1226. <http://hdl.handle.net/2027.42/65285> | en_US |
dc.identifier.issn | 0022-3042 | en_US |
dc.identifier.issn | 1471-4159 | en_US |
dc.identifier.uri | https://hdl.handle.net/2027.42/65285 | |
dc.identifier.uri | http://www.ncbi.nlm.nih.gov/sites/entrez?cmd=retrieve&db=pubmed&list_uids=3346675&dopt=citation | en_US |
dc.description.abstract | Regional transport of 1-aminocyclohexanecarboxylic acid (ACHC), a nonmetabolizable amino acid, across the blood-brain barrier was studied in pentobarbital-anesthetized rats using an in situ brain perfusion technique. The concentration dependence of influx was best described by a model with a saturable and a nonsaturable component. Best-fit values for the kinetic constants of the frontal cortex equaled 9.7 × 10- −4 Μmol/s/g for V max , 0.054 Μmol/ml for K m , and 1.0 × 10- −4 ml/s/g for K D in the absence of competing amino acids. Saturable influx could be reduced by >85% by either l-phenylalanine or 2-aminobicyclo[2.2.1]heptane-2-carboxylic acid, consistent with transport by the cerebrovascular neutral amino acid transport system. The transport K m for ACHC was one-fifth that for the more commonly used homologue, I-aminocyclopentanecarboxylic acid, and was similar to values for several natural amino acids, such as l-methionine, l-isoleucine, and l-tyrosine. The results indicate that ACHC may be a useful probe for in vivo studies of amino acid transport into brain. | en_US |
dc.format.extent | 745787 bytes | |
dc.format.extent | 3110 bytes | |
dc.format.mimetype | application/pdf | |
dc.format.mimetype | text/plain | |
dc.publisher | Blackwell Publishing Ltd | en_US |
dc.rights | 1988 International Society for Neurochemistry | en_US |
dc.subject.other | Amino Acid | en_US |
dc.subject.other | Blood-brain Barrier | en_US |
dc.subject.other | Transport | en_US |
dc.subject.other | Capillary | en_US |
dc.subject.other | Brain | en_US |
dc.subject.other | Rat | en_US |
dc.title | Blood-Brain Barrier Transport of 1-Aminocyclohexanecarboxylic Acid, a Nonmetabolizable Amino Acid for In Vivo Studies of Brain Transport | en_US |
dc.type | Article | en_US |
dc.rights.robots | IndexNoFollow | en_US |
dc.subject.hlbsecondlevel | Neurosciences | en_US |
dc.subject.hlbtoplevel | Health Sciences | en_US |
dc.description.peerreviewed | Peer Reviewed | en_US |
dc.contributor.affiliationum | * Neuroscience Laboratory, University of Michigan, Ann Arbor, Michigan | en_US |
dc.contributor.affiliationother | Laboratory of Neurosciences, National Institute on Aging, National Institutes of Health, Bethesda, Maryland | en_US |
dc.contributor.affiliationother | Medical and Health Sciences Division, Oak Ridge Associated Universities, Oak Ridge, Tennessee, U.S.A | en_US |
dc.identifier.pmid | 3346675 | en_US |
dc.description.bitstreamurl | http://deepblue.lib.umich.edu/bitstream/2027.42/65285/1/j.1471-4159.1988.tb10596.x.pdf | |
dc.identifier.doi | 10.1111/j.1471-4159.1988.tb10596.x | en_US |
dc.identifier.source | Journal of Neurochemistry | en_US |
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dc.owningcollname | Interdisciplinary and Peer-Reviewed |
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