Glutamate-Stimulated, Guanine Nucleotide-Mediated Phosphoinositide Turnover in Astrocytes Is Inhibited by Cyclic AMP
dc.contributor.author | Robertson, Patricia L. | en_US |
dc.contributor.author | Bruno, George R. | en_US |
dc.contributor.author | Datta, Subhash C. | en_US |
dc.date.accessioned | 2010-04-01T14:50:16Z | |
dc.date.available | 2010-04-01T14:50:16Z | |
dc.date.issued | 1990-11 | en_US |
dc.identifier.citation | Robertson, Patricia L.; Bruno, George R.; Datta, Subhash C. (1990). "Glutamate-Stimulated, Guanine Nucleotide-Mediated Phosphoinositide Turnover in Astrocytes Is Inhibited by Cyclic AMP." Journal of Neurochemistry 55(5): 1727-1733. <http://hdl.handle.net/2027.42/65289> | en_US |
dc.identifier.issn | 0022-3042 | en_US |
dc.identifier.issn | 1471-4159 | en_US |
dc.identifier.uri | https://hdl.handle.net/2027.42/65289 | |
dc.identifier.uri | http://www.ncbi.nlm.nih.gov/sites/entrez?cmd=retrieve&db=pubmed&list_uids=1976758&dopt=citation | en_US |
dc.description.abstract | The potential for cross-talk between the adenyl cy-clase and phosphoinositide (PPI) lipid second messenger system was investigated in astrocytes cultured from neonatal rat brain. Glutamate-stimulated PPI turnover, measured by the formation of total inositol phosphates from myo -[ 3 H]inositoI-labeled lipids, was inhibited in a concentration-dependent manner by the elevation of intracellular cyclic AMP levels produced either by stimulation of the isoproter-enol receptor linked to adenyl cyclase or by its direct activation by forskolin. N 6 ,2′- O -Dibutyryl cyclic AMP, an analogue that can also activate cyclic AMP-dependent kinase, inhibited glutamate-stimulated PPI turnover in a concentration-dependent manner as well, a result suggesting that cyclic AMP-dependent kinase is involved in mediating the inhibition. Inclusion of an inhibitor of cyclic AMP-dependent kinase, l-(5-isoquinolinesulfonyl)-2 methylpiperazine dihy-drochloride or N -(2-guanidinoethyl)-5-isoquinolinesulfon-amide hydrochloride, blocked the cyclic AMP-mediated inhibition in a concentration-dependent manner, a finding further supporting this hypothesis. The site of inhibition of the phosphoinositol lipid pathway by cyclic AMP was probed using a digitonin-permeabilized cell system. Guanosine 5′- O -(3-thiotriphosphate), a nonhydrolyzable analogue of GTP, stimulated PPI turnover and potentiated glutamate-stimulated PPI turnover, and guanosine 5′- O -(3-thiodiphosphate) inhibited glutamate-stimulated PPI turnover in these cells, results providing evidence that glutamate receptors are coupled to phospholipase C by a guanine nucleotide binding protein in astrocytes. N 6 ,2′- O -Dibutyryl cyclic AMP and agents that elevate cyclic AMP levels inhibited the PPI turnover stimulated by guanosine 5′- O -(3-thiotriphosphate), as well as that potentiated by guanosine 5′- O -(3-thiotriphos-phate) in the presence of glutamate, results suggesting that the cyclic AMP-dependent inhibition occurs at or distal to the putative guanine nucleotide binding protein. Because basal PPI turnover was not altered by elevation of cyclic AMP levels, direct inhibition of phospholipase C is unlikely. | en_US |
dc.format.extent | 838217 bytes | |
dc.format.extent | 3110 bytes | |
dc.format.mimetype | application/pdf | |
dc.format.mimetype | text/plain | |
dc.publisher | Blackwell Publishing Ltd | en_US |
dc.rights | 1990 International Society for Neurochemistry | en_US |
dc.subject.other | Glutamate | en_US |
dc.subject.other | Adenyl Cyclase | en_US |
dc.subject.other | Cyclic AMP | en_US |
dc.subject.other | Phos-phoinositides | en_US |
dc.subject.other | GTP Binding Protein | en_US |
dc.title | Glutamate-Stimulated, Guanine Nucleotide-Mediated Phosphoinositide Turnover in Astrocytes Is Inhibited by Cyclic AMP | en_US |
dc.type | Article | en_US |
dc.rights.robots | IndexNoFollow | en_US |
dc.subject.hlbsecondlevel | Neurosciences | en_US |
dc.subject.hlbtoplevel | Health Sciences | en_US |
dc.description.peerreviewed | Peer Reviewed | en_US |
dc.contributor.affiliationum | Departments of Pediatrics and Neurology, The University of Michigan, Ann Arbor, Michigan, U.S.A. | en_US |
dc.identifier.pmid | 1976758 | en_US |
dc.description.bitstreamurl | http://deepblue.lib.umich.edu/bitstream/2027.42/65289/1/j.1471-4159.1990.tb04962.x.pdf | |
dc.identifier.doi | 10.1111/j.1471-4159.1990.tb04962.x | en_US |
dc.identifier.source | Journal of Neurochemistry | en_US |
dc.identifier.citedreference | Akil M. and Fisher S. K. ( 1989 ) Muscarinic receptor-stimulated phosphoinositide turnover in human SK-N-SH neuroblastoma cells: differential inhibition by agents that elevate cyclic AMP. J. Neurochem. 53, 1479 – 1486. | en_US |
dc.identifier.citedreference | Bowman C. L. and Kimelberg H. K. ( 1984 ) Excitatory amino acids directly depolarize rat brain astrocytes in primary culture. Nature 311, 656 – 659. | en_US |
dc.identifier.citedreference | Brass L. F., Laposata M., Banga H. S., and Rittenhouse S. ( 1986 ) Regulation of the phosphoinositide hydrolysis pathway by a pertussis toxin-sensitive guanine nucleotide-binding protein. J. Biol. Chem. 261, 16838 – 16847. | en_US |
dc.identifier.citedreference | Cockcroft S. and Gomperts B. D. ( 1985 ) Role of guanine nucleotide binding protein in the activation of phosphoinositide phosphodiesterase. Nature 314, 534 – 536. | en_US |
dc.identifier.citedreference | Cummins C. J., Lust W. D., and Passoneau J. V. ( 1983 ) Regulation of glycogenolysis in transformed astrocytes in vitro. J. Neurochem. 40, 137 – 144. | en_US |
dc.identifier.citedreference | Drejer J., Larsson O. M., and Schousboe A. ( 1982 ) Characterization of L-glutamate uptake into and release from astrocytes and neurons cultured from different brain regions. Exp. Brain Res. 47, 259 – 269. | en_US |
dc.identifier.citedreference | Eberhard D. A. and Holz R. W. ( 1987 ) Cholinergic stimulation of inositol phosphate formation in bovine adrenal chromaffin cells: distinct nicotinic and muscarinic mechanisms. J. Neurochem. 49, 1634 – 1643. | en_US |
dc.identifier.citedreference | Eckstein F., Cassel D., Levkovitz H., Lowe M., and Selinger Z. ( 1979 ) Guanosine 5prime;- O -(2-thiodiphosphate), an inhibitor of adenylate cyclase stimulation by guanine nucleotides and fluoride ions. J. Biol. Chem. 254, 9829 – 9834. | en_US |
dc.identifier.citedreference | Evans T., McCarthy K. D., and Harden T. K. ( 1984 ) Regulation of cyclic AMP accumulation by peptide hormone eceptors in immunocytochemically defined astroglial cells. J. Neurochem. 43, 131 – 138. | en_US |
dc.identifier.citedreference | Fisher S. K., Domask L. M., and Roland R. M. ( 1989 ) Muscarinic receptor regulation of cytoplasmic Ca 2+ concentrations in human SK-N-SH neuroblastoma cells: Ca 24 requirements for phospholipase C activation. Mol. Pharmacol. 35, 195 – 204. | en_US |
dc.identifier.citedreference | Francel P. C., Miller R. J., and Dawson G. ( 1987 ) Modulation of bradykinin-induced inositol triphosphate release in a novel neuroblastoma dorsal root ganglion sensory neuron cell line. J. Neurochem. 48, 1632 – 1639. | en_US |
dc.identifier.citedreference | Frangakis M. V. and Kimelberg H. K. ( 1984 ) Dissociation of neonatal rat brain by dispase for preparation of primary astrocyte cultures. Neurochem. Res. 9, 1689 – 1698. | en_US |
dc.identifier.citedreference | Guillon G., Gallo-Payet N., Balestre M. N., and Lombard C. ( 1988 ) Cholera-toxin and corticotropin modulation of inositol phosphate accumulation induced by vasopressin and angiotensin II in rat glomerulosa cells. Biochem. J. 253, 765 – 775. | en_US |
dc.identifier.citedreference | Henn F. A. and Henn S. W. ( 1980 ) The psychopharmacology of astroglial cell. Prog. Neurobiol. 15, 1 – 17. | en_US |
dc.identifier.citedreference | Hidaka H., Inagaki M., Kawamoto S., and Sasaki V. ( 1984 ) lsoquinoline-sulfonamides, novel and potent inhibitors of cyclic nucleotide dependent protein kinase and protein kinase C. Biochemistry 23, 5036 – 5041. | en_US |
dc.identifier.citedreference | Hill S. J. and Kendall D. A. ( 1987 ) Studies on the adenosine-receptor mediating the augmentation of histamine-induced inositol phospholipid hydrolysis in guinea-pig cerebral cortex. Br. J. Pharmacol. 91, 661 – 669. | en_US |
dc.identifier.citedreference | Kaibuchi K., Takai Y., Ogawa Y., Kimura S., and Nishizuka Y. ( 1982 ) Inhibitory action of adenosine 3prime;,5prime;-monophosphate on phosphatidyl-inositol turnover: difference in tissue response. Biochem. Biophys. Res. Commun. 104, 105 – 112. | en_US |
dc.identifier.citedreference | Kettenmann H. and Schachner M. ( 1985 ) Pharmacological properties of Γ-aminobutyric acid, glutamate, and aspartate-induced depolarizations in cultured astrocytes. J. Neurosci. 5, 3295 – 3301. | en_US |
dc.identifier.citedreference | Kim U. H., Kim J. W., and Rhee S. G. ( 1989 ) Phosphorylation of phospholipase C-Γ by cAMP-dependent protein kinase. J. Biol. Chem. 264, 20169 – 20170. | en_US |
dc.identifier.citedreference | Kimelberg H. K., Bowman C. L., and Hirata H. ( 1986 ) Anion transport in astrocytes. Ann. NY Acad. Sci. 481, 334 – 353. | en_US |
dc.identifier.citedreference | Lapetina E. G., Local J. C., Reep B. R., and Molinay Vedia L. ( 1989 ) A ras -related protein in phosphorylated and tranlocated by agonists that increase cAMP levels in human platelets. Proc. Natl. Acad. Sci. USA 86, 3131 – 3134. | en_US |
dc.identifier.citedreference | Laufer R. and Changeux J. ( 1989 ) Calcitonin gene-related peptide and cyclic AMP stimulate phosphoinositide turnover in skeletal muscle cells. J. Biol. Chem. 264, 2683 – 2689. | en_US |
dc.identifier.citedreference | Lazarowski E. R. and Lapetina E. G. ( 1989 ) Activation of platelet phospholipase C by fluoride is inhibited by cyclic AMP. Biochem. Biophys. Res. Commun. 158, 440 – 444. | en_US |
dc.identifier.citedreference | Lerea K. M., Glomset J. A., and Krebs E. G. ( 1987 ) Agents that elevate cAMP levels in platelets decrease thrombin binding. J. Biol. Chem. 262, 282 – 288. | en_US |
dc.identifier.citedreference | Madison J. M. and Brown J. K. ( 1988 ) Differential inhibitory effects of forskolin, isoproterenol. and dibutyryl cyclic adenosine monophosphate on phosphoinositide hydrolysis in canine tracheal smooth muscle. J. Clin. Invest. 82, 1462 – 1465. | en_US |
dc.identifier.citedreference | McCarthy K. D. and de Vellis J. ( 1978 ) Alpha-adrenergic receptor modulation of beta-adrenergic. adenosine. and prostaglandin E 1 increased adenosine 3prime;:5prime;-cyciic monophosphate levels in primary cultures of astrocytes. J. Cyclic Nucleotide Res. 4, 15 – 26. | en_US |
dc.identifier.citedreference | Paris S. and Pouyssegur J. ( 1987 ) Further evidence fora phospholipase C-coupled G protein in hamster fibroblasts. Induction of inositol phosphate formation by fluoroaluminate and vanadate and inhibition by pertussis toxin. J. Biol. Chem. 262, 1970 – 1976. | en_US |
dc.identifier.citedreference | Pearce B., Albrecht J., Morrow C., and Murphy S. ( 1986a ) Astrocyte glutamate receptor activation promotes inositol phospholipid turnover and calcium flux. Neurosci. Lett. 72, 335 – 340. | en_US |
dc.identifier.citedreference | Pearce B., Morrow C., and Murphy S. ( 1986b ) Receptor-mediated inositol phospholipid hydrolysis in astrocytes. Eur. J. Pharmacol. 121, 231 – 243. | en_US |
dc.identifier.citedreference | Pearce B., Morrow C., and Murphy S. ( 1988 ) Characteristics of phorbol ester- and agonist-induced down-regulation of astrocytes coupled to inositol phospholipid metabolism. J. Neurochem. 50, 936 – 944. | en_US |
dc.identifier.citedreference | Portzehl H., Caldwell P. C. and Reugg J. C. ( 1964 ) The dependence of contraction and relaxation of muscle fibers from the crab Maia squmado on the internal concentration of free calcium ions. Biochim. Biophys. Acta 79, 581 – 591. | en_US |
dc.identifier.citedreference | Puurunen J., Lohse M. J., and Schwabe U. ( 1987 ) Interactions between intracellular cyclic AMP and agonist-induced inositol phospholipid breakdown in isolated gastric mucosal cells of the rat. Naunyn Schmiedehergs Arch. Pharmacol. 336, 471 – 477. | en_US |
dc.identifier.citedreference | Ritchie T., Cole R., Kim H. S., de Vellis J., and Noble E. P. ( 1987 ) Inositol phospholipid hydrolysis in cultured astrocytes and oligodendrocytes. Life Sci. 41, 31 – 39. | en_US |
dc.identifier.citedreference | Robertson P. L. and Goldstein G. W. ( 1988 ) Heparin inhibits the growth of astrocytes in vitro. Brain Res. 447, 341 – 345. | en_US |
dc.identifier.citedreference | Robertson P. L., Markovac J., Datta S. C. and Goldstein G. W. ( 1988 ) Transforming growth factor beta stimulates phosphoinositol metabolism and translocation of protein kinase C in cultured astrocytes. Neurosci. Lett. 93, 107 – 113. | en_US |
dc.identifier.citedreference | Seamon K. B. and Daly J. W. ( 1986 ) Forskolin: its biological and chemical properties. Adv. Cyclic Nucleotide Protein Phosphorylation Res. 20, 1 – 150. | en_US |
dc.identifier.citedreference | Shain W., Madelian V., Martin D. L., Kimelberg H. K., Perrone M., and Lepore R. ( 1986 ) Activation of Β-adrenergic receptors stimulates release of an inhibitory transmitter from astrocytes. J. Neurochem. 46, 1298 – 1303. | en_US |
dc.identifier.citedreference | Takenawa T., Ishitoya J., and Nagai Y. ( 1986 ) Inhibitory effect of prostaglandin E 2, forskolin. and dibutyryl cAMP on arachidonic acid release and inositol phospholipid metabolism in guinea pig neutrophils. J. Biol. Chem. 261, 1092 – 1098. | en_US |
dc.identifier.citedreference | Van Calker D., Loftier F., and Hamprecht B. ( 1983 ) Corticotropin peptides and melanotropins elevate the level of adenosine 3prime;:5prime;-cyclic monophosphate in cultured murine brain cells. J. Neurochem. 40, 418 – 427. | en_US |
dc.identifier.citedreference | Windebank K. P., Abraham R. T., Powis G., Olsen R. A., Barna T. J., and Leibson P. J. ( 1988 ) Signal transduction during human natural killer cell activation: inositol phosphate generation and regulation by cyclic AMP. J. Immunol. 141, 3951 – 3957. | en_US |
dc.identifier.citedreference | Yada Y., Nagao S., Okano Y., and Nozawa Y. ( 1989 ) Inhibition by cyclic AMP of guanine nucleotide-induced activation of phosphoinositide-specific phospholipase C in human platelets. IEBS Lett. 242, 368 – 372. | en_US |
dc.owningcollname | Interdisciplinary and Peer-Reviewed |
Files in this item
Remediation of Harmful Language
The University of Michigan Library aims to describe library materials in a way that respects the people and communities who create, use, and are represented in our collections. Report harmful or offensive language in catalog records, finding aids, or elsewhere in our collections anonymously through our metadata feedback form. More information at Remediation of Harmful Language.
Accessibility
If you are unable to use this file in its current format, please select the Contact Us link and we can modify it to make it more accessible to you.