Formation of Free Fatty Acid and Ceramide During Brain Handling: Lability of Sphingomyelin
dc.contributor.author | Deshmukh, Gayatri D. | en_US |
dc.contributor.author | Radin, Norman S. | en_US |
dc.date.accessioned | 2010-04-01T14:50:39Z | |
dc.date.available | 2010-04-01T14:50:39Z | |
dc.date.issued | 1985-04 | en_US |
dc.identifier.citation | Deshmukh, Gayatri D.; Radin, Norman S. (1985). "Formation of Free Fatty Acid and Ceramide During Brain Handling: Lability of Sphingomyelin." Journal of Neurochemistry 44(4): 1152-1155. <http://hdl.handle.net/2027.42/65296> | en_US |
dc.identifier.issn | 0022-3042 | en_US |
dc.identifier.issn | 1471-4159 | en_US |
dc.identifier.uri | https://hdl.handle.net/2027.42/65296 | |
dc.identifier.uri | http://www.ncbi.nlm.nih.gov/sites/entrez?cmd=retrieve&db=pubmed&list_uids=2983027&dopt=citation | en_US |
dc.description.abstract | Intact brain and brain homogenates readily form free fatty acids and ceramides, even in the cold during subcellular isolation procedures. The fatty acid formation is slightly stimulated by chelators and might be due to phospholipid hydrolysis by lysosomal phospholipases. The ceramide formation is accompanied by loss of sphingomyelin and is apparently due to the action of neutral, metal ion-activated sphingomyelinase. The latter reaction is inhibited by EDTA whereas both degradative processes are inhibited by mercuriphenylsulfonate, the thiol-reacting inhibitor. Cerebroside does not seem to be a source of accumulated ceramide. | en_US |
dc.format.extent | 405640 bytes | |
dc.format.extent | 3110 bytes | |
dc.format.mimetype | application/pdf | |
dc.format.mimetype | text/plain | |
dc.publisher | Blackwell Publishing Ltd | en_US |
dc.rights | 1985 International Society for Neurochemistry | en_US |
dc.subject.other | Sphin Gomyelinase | en_US |
dc.subject.other | Galactocerebrosidase | en_US |
dc.subject.other | Ceramide Formation | en_US |
dc.subject.other | Free Fatty Acid Formation | en_US |
dc.title | Formation of Free Fatty Acid and Ceramide During Brain Handling: Lability of Sphingomyelin | en_US |
dc.type | Article | en_US |
dc.rights.robots | IndexNoFollow | en_US |
dc.subject.hlbsecondlevel | Neurosciences | en_US |
dc.subject.hlbtoplevel | Health Sciences | en_US |
dc.description.peerreviewed | Peer Reviewed | en_US |
dc.contributor.affiliationum | Mental Health Research Institute and Department of Biological Chemistry, The University of Michigan, Ann Arbor, Michigan, U.S.A | en_US |
dc.identifier.pmid | 2983027 | en_US |
dc.description.bitstreamurl | http://deepblue.lib.umich.edu/bitstream/2027.42/65296/1/j.1471-4159.1985.tb08737.x.pdf | |
dc.identifier.doi | 10.1111/j.1471-4159.1985.tb08737.x | en_US |
dc.identifier.source | Journal of Neurochemistry | en_US |
dc.identifier.citedreference | Arora R. C., Lin Y.-N., and Radin N. S. ( 1973 ) The inhibitor sensitive sites of galactosyl ceramide galactosidase. Arch. Biochem. Biophys. 156, 77 – 83. | en_US |
dc.identifier.citedreference | Bazan N. G. ( 1970 ) Effects of ischemia and electroconvulsive shock on free fatty acid pool in the brain. Biochim. Biophys. Acta 218, 1 – 10. | en_US |
dc.identifier.citedreference | Cenedella R. J., Galli C., and Paoletti R. ( 1975 ) Brain free fatty acid levels in rats sacrificed by decapitation versus focused microwave irradiation. Lipids 10, 290 – 293. | en_US |
dc.identifier.citedreference | Gatt S. ( 1966 ) Enzymatic hydrolysis of sphingolipids. Hydrolysis and synthesis of ceramides by an enzyme from rat brain. J. Biol. Chem. 241, 3724 – 3730. | en_US |
dc.identifier.citedreference | Gatt S. ( 1982 ) Studies on sphingomyelinase, in Phospholipids in the Nervous System, Vol. 1: Metabolism ( Horrocks L. A., Ansell G. B., and Porcellati G., eds ), pp. 181 – 197. Raven Press, New York. | en_US |
dc.identifier.citedreference | Gatt S. and Bierman E. L. ( 1980 ) Sphingomyelin suppresses the binding and utilization of low density lipoproteins by skin fibroblasts. J. Biol. Chem. 255, 3371 – 3376. | en_US |
dc.identifier.citedreference | Hostetler K. Y., Yazaki P. J., and van den Bosch H. ( 1982 ) Purification of lysosomal phospholipase A. Evidence for multiple isoenzymes in rat liver. J. Biol. Chem. 257, 13367 – 13373. | en_US |
dc.identifier.citedreference | Lunt G. G. and Rowe C. E. ( 1968 ) The production of unesterified fatty acid in brain. Biochim. Biophys. Acta 152, 681 – 693. | en_US |
dc.identifier.citedreference | Majewska M. D., Manning R., and Sun, G. Y. ( 1981 ) Effects of postdecapitative ischemia on arachidonate release from brain synaptosomes. Neurochem. Res. 6, 567 – 576. | en_US |
dc.identifier.citedreference | Pediconi M. F., Rodriguez de Turco E. B., and Bazan N. G. ( 1981 ) Metabolism of arachidonic acid in the neural tissue, in New Trends in Nutrition, Lipid Research, and Cardiovascular Diseases ( Bazan N. G., Paoletti R., and Iacono J. M., eds ), pp. 17 – 23. Alan R. Liss, New York. | en_US |
dc.identifier.citedreference | Radin N. S. ( 1981 ) Extraction of tissue lipids with a solvent of low toxicity. Methods Enzymol. 72, 5 – 7. | en_US |
dc.identifier.citedreference | Radin N. S., Deshmukh G. D., Selvam R., and Hospattankar A. V. ( 1982 ) Determination of glucocerebroside, sphingomyelin, free fatty acid and total lipid by thin-layer chromatography and charring-scintillation quenching. Biochim. Biophys. Acta 713, 474 – 478. | en_US |
dc.identifier.citedreference | Rao B. G. and Spence M. W. ( 1976 ) Sphingomyelinase activity at pH 7.4 in human brain and a comparison to activity at pH 5.0 J. Lipid Res. 17, 506 – 515. | en_US |
dc.identifier.citedreference | Record M., Loyter A., and Gatt S. ( 1980 ) Utilization of membranous lipid substrates by membranous enzymes. Biochem. J. 187, 115 – 121. | en_US |
dc.identifier.citedreference | Rodriguez de Turco E. B., Morelli de Liberti S., and Bazan N. G. ( 1983 ) Stimulation of free fatty acid and diacylglycerol accumulation in cerebrum and cerebellum during bicuculline-induced status epilepticus. Effect of pretreatment with Α-methyl- p -tyrosine and p -chlorophenylalanine. J. Neurochem. 40, 252 – 259. | en_US |
dc.identifier.citedreference | Selvam R. and Radin N. S. ( 1981 ) Quantitation of lipids by charring on thin-layer plates and scintillation quenching: application to ceramide determination. Anal. Biochem. 112, 338 – 345. | en_US |
dc.identifier.citedreference | Sugita M., Williams M., Dulaney J. T., and Moser H. W. ( 1975 ) Ceramidase and ceramide synthesis in human kidney and cerebellum. Description of a new alkaline ceramidase. Biochim. Biophys. Acta 398, 125 – 131. | en_US |
dc.identifier.citedreference | Watkins M. S., Hitt A. S., and Bulger J. E. ( 1977 ) The binding of 18S acetylcholinesterase to sphingomyelin and the role of the collagen-like tail. Biochem. Biophys. Res. Commun. 79, 640 – 647. | en_US |
dc.identifier.citedreference | Yamanaka T., Manada E., and Suzuki, K. ( 1981 ) Acid sphingomyelinase of human brain. J. Biol. Chem. 256, 3884 – 3889. | en_US |
dc.owningcollname | Interdisciplinary and Peer-Reviewed |
Files in this item
Remediation of Harmful Language
The University of Michigan Library aims to describe library materials in a way that respects the people and communities who create, use, and are represented in our collections. Report harmful or offensive language in catalog records, finding aids, or elsewhere in our collections anonymously through our metadata feedback form. More information at Remediation of Harmful Language.
Accessibility
If you are unable to use this file in its current format, please select the Contact Us link and we can modify it to make it more accessible to you.