Show simple item record

Goldfish Brain (Na + , K + )-ATPase: Purification of the Catalytic Polypeptide and Production of Specific Antibodies

dc.contributor.authorSchwartz, Michalen_US
dc.contributor.authorSiegel, George J.en_US
dc.contributor.authorChen, Nelsonen_US
dc.contributor.authorAgranoff, Bernard W.en_US
dc.date.accessioned2010-04-01T14:52:28Z
dc.date.available2010-04-01T14:52:28Z
dc.date.issued1980-06en_US
dc.identifier.citationSchwartz, Michal; Siegel, George J.; Chen, Nelson; Agranoff, Bernard W. (1980). "Goldfish Brain (Na + , K + )-ATPase: Purification of the Catalytic Polypeptide and Production of Specific Antibodies." Journal of Neurochemistry 34(6): 1745-1752. <http://hdl.handle.net/2027.42/65328>en_US
dc.identifier.issn0022-3042en_US
dc.identifier.issn1471-4159en_US
dc.identifier.urihttps://hdl.handle.net/2027.42/65328
dc.identifier.urihttp://www.ncbi.nlm.nih.gov/sites/entrez?cmd=retrieve&db=pubmed&list_uids=6155444&dopt=citationen_US
dc.description.abstractThe denatured catalytic polypeptide of (Na + , K + )-ATPase of goldfish brain was purified and identified as the 32 P-labeled phosphoprotein. The protein served as immunogen for the preparation of rabbit antisera for immunohistochemical application to goldfish tissue sections, using the peroxidase-antiperoxidase indirect method. Labeling in brain cross-sections appears primarily in fibers of the optic nerve layer of the tectum. In optic nerve cross sections, labeling is restricted to fiber bundles.en_US
dc.format.extent846606 bytes
dc.format.extent3110 bytes
dc.format.mimetypeapplication/pdf
dc.format.mimetypetext/plain
dc.publisherBlackwell Publishing Ltden_US
dc.rights1980 International Society for Neurochemistryen_US
dc.subject.otherGoldfishen_US
dc.subject.other(Na + , K + )-ATPaseen_US
dc.subject.otherImmunocytochemistryen_US
dc.subject.otherVisual Systemen_US
dc.subject.otherOptic Nerveen_US
dc.titleGoldfish Brain (Na + , K + )-ATPase: Purification of the Catalytic Polypeptide and Production of Specific Antibodiesen_US
dc.typeArticleen_US
dc.rights.robotsIndexNoFollowen_US
dc.subject.hlbsecondlevelNeurosciencesen_US
dc.subject.hlbtoplevelHealth Sciencesen_US
dc.description.peerreviewedPeer Revieweden_US
dc.contributor.affiliationumDepartments of Biological Chemistry, Neurology and Mental Health Research Institute, University oj Michigan, Ann Arbor, Michigan 48109, U.S.A.en_US
dc.identifier.pmid6155444en_US
dc.description.bitstreamurlhttp://deepblue.lib.umich.edu/bitstream/2027.42/65328/1/j.1471-4159.1980.tb11269.x.pdf
dc.identifier.doi10.1111/j.1471-4159.1980.tb11269.xen_US
dc.identifier.sourceJournal of Neurochemistryen_US
dc.identifier.citedreferenceAskari A. and Rao S. N. ( 1972 ) (Na +, K + )-ATPase complex: Effects of anticomplex antibody on the partial reactions catalysed by the complex. Biochem. Biophys. Res. Commun. 49, 1323 – 1328.en_US
dc.identifier.citedreferenceBanga J. P., Anderton B. H. and Roitt I. M. ( 1978 ) Separation of membrane proteins in an undenatured form by isoelectric focusing. Anal. Biochem. 89, 348 – 354.en_US
dc.identifier.citedreferenceBertoni J. B. and Siegel G. J. ( 1978 ) Development of (Na + -K + )-ATPase in rat cerebrum: Correlation with Na + -dependent phosphorylation and K + -paranitrophenylphosphatase. J. Neurochem. 31, 1501 – 1511.en_US
dc.identifier.citedreferenceBertoni J. B. and Siegel G. J. ( 1979 ) Development of (Na + -K+)-ATPase in rat hindbrain-increments in parallel with Na + -dependent phosphorylation and K + -paranitrophenyl-phosphatase. J. Neurochem. 32, 573 – 580.en_US
dc.identifier.citedreferenceBroderson S. H., Patton D. L. and Stahl W. L. ( 1978 ) Fine structural localization of potassium stimulated p -nitrophenyl-phosphatase activity in dendrites of cerebral cortex. J. Cell Biol. 88, R13 – R17.en_US
dc.identifier.citedreferenceBurrell H. R., Heacock A. M., Water R. D. and Agranoff B. W. ( 1978 ) Increased tubulin messenger RNA in the goldfish retina during optic nerve regeneration. Brain Res. 168, 628 – 632.en_US
dc.identifier.citedreferenceDahl J. L. and Hokin L. E. ( 1974 ) Sodium-potassium adenosinetriphosphatase. Anna. Rev. Biochem. 43, 327 – 356.en_US
dc.identifier.citedreferenceDanno G. ( 1977 ) Isoelectric focusing of proteins separated by SDS-polyacrylamide gel electrophoresis. Anal. Biochem. 83, 189 – 193.en_US
dc.identifier.citedreferenceDixon J. F. and Hokin L. E. ( 1974 ) Studies on characterization of sodium potassium transport adenosinetriphosphatase. 16. Purification and properties of enzyme from electric organ of Electrophorus electricus. Arch. Biochem. Biophys. 163, 749 – 758.en_US
dc.identifier.citedreferenceGaze R. M. ( 1970 ) The Formation of Nerve Connections. Academic Press, London.en_US
dc.identifier.citedreferenceGlynn I. M. and Karlish S. J. D. ( 1975 ) The sodium pump. Annu. Rev. Physiol. 37, 13 – 55.en_US
dc.identifier.citedreferenceGrafstein B. ( 1975 ) The nerve cell body response to axotomy. Exp. Neurobiol. 48, 32 – 51.en_US
dc.identifier.citedreferenceGuth L. and Albers R. W. ( 1974 ) Histochemical demonstration of (Na +, K + )-activated adenosine triphosphatase. J. Histochem. Cytochem. 22, 320 – 326.en_US
dc.identifier.citedreferenceHokin L. E., Dahl J. L., Deupree J. D., Dixon J. F., Hackney J. F. and Perdue J. F. ( 1973 ) Studies on the characterization of sodium-potassium transport adenosine triphosphatase. X. Purification of the enzyme from rectal gland of Squalus acanthias. J. Biol. Chem. 248, 2593 – 2605.en_US
dc.identifier.citedreferenceHopkins B. E., Wagner H., Jr. and Smith T. W. ( 1976 ) Sodium and potassium-activated adenosine triphosphatase of the nasal salt gland of the duck ( Anas platyrhynchos ). J. Biol. Chem. 251, 4365 – 4371.en_US
dc.identifier.citedreferenceJacobson M. and Gaze R. M. ( 1965 ) Selection of appropriate tectal connections by regenerating optic nerve fibers in adult goldfish. Exp. Neurol. 13, 418 – 430.en_US
dc.identifier.citedreferenceJean D. H. and Albers R. W. ( 1976 ) Immunochemical studies on the large polypeptide of Electrophorus electroplax (Na + + K + -ATPase. Biochem. Biophys. Acta 452, 219 – 226.en_US
dc.identifier.citedreferenceJean D. H., Albers R. W. and Koval G. J. ( 1975 ) Sodium-potassium activated adenosine triphosphatase of Electrophorus electric organ. 10. Immunochemical properties of the lubrol-solubilized enzyme and its constituents. J. Biol. Chem. 250, 1035 – 1040.en_US
dc.identifier.citedreferenceJorgensen P. L. ( 1974 ) Purification and characterization of (Na + + K + )-ATPase. IV. Estimation of the purity and of the molecular weight and polypeptide content per enzyme unit in preparation from the outer medulla of rabbit kidney. Biochem. Biophys. Acta 356, 53 – 67.en_US
dc.identifier.citedreferenceKappers C. U. A., Huber G. C. and Crosby E. C. ( 1960 ) The Comparative Anatomy of the Nervous System of Vertebrates Including Man, Vol. II. Hafner Publishing Company, New York.en_US
dc.identifier.citedreferenceKlodos I., Ottolenghi P. and Boldyrev A. ( 1975 ) Large scale preparation of (Na +, K + )-ATPase from ox brain. Anal. Biochem. 67, 397 – 403.en_US
dc.identifier.citedreferenceKyte J. ( 1971 ) Purification of the sodium- and potassium-dependent adenosine triphosphatase from canine renal medulla. J. Biol. Chem. 246, 4157 – 4165.en_US
dc.identifier.citedreferenceLandreth G. E. and Agranoff B. W. ( 1979 ) Explant culture of adult goldfish retina: A model for the study of CNS regeneration. Brain Res. 161, 39 – 53.en_US
dc.identifier.citedreferenceLane L. K., Potter J. D. and Collins J. H. ( 1979 ) Large scale purification of Na, K-ATPase and its protein subunits from lamb kidney medulla. Prep. Biochem. 9, 157 – 170.en_US
dc.identifier.citedreferenceLowry O. H., Rosebrough N. J., Fair A. L. and Randall R. J. ( 1951 ) Protein measurement with the Folin phenol reagent. J. Biol. Chem. 193, 265 – 275.en_US
dc.identifier.citedreferenceMeek J. and Schellart N. A. M. ( 1978 ) A Golgi study of goldfish optic tectum. J. Comp. Neural. 182, 89 – 122.en_US
dc.identifier.citedreferenceNakao T., Tashuma Y., Nagano K. and Nakao M. ( 1965 ) Highly specific sodium-potassium activated adenosine triphosphatase from various tissues of rabbit. Biochem. Biophys. Res. Commun. 19, 755 – 758.en_US
dc.identifier.citedreferencePeter R. E. and Gill V. E. ( 1975 ) A stereotaxic atlas and technique for forebrain nuclei of the goldfish, Carrasius auratus. J. Comp. Neural. 159, 69 – 102.en_US
dc.identifier.citedreferenceSchwartz A., Lindenmayer G. E. and Allen J. C. ( 1975 ) The sodium potassium adenosine triphosphatase pharmacological, physiological and biochemical aspects. Pharmacol. Rev. 27, 3 – 134.en_US
dc.identifier.citedreferenceSchwartz M., Feldman E. L., Jean D. H., Siegel G. J. and Agranoff B. W. ( 1979 ) Na-K-ATPase in the regenerating goldfish visual system. Trans. Am. Soc. Neurochem. 10, 233.en_US
dc.identifier.citedreferenceSiegel G. J. and Albers R. W. ( 1967 ) Sodium potassium activated adenosine triphosphatase of Electrophorus electric organ. IV. Modification of response to sodium and potassium by arsenite plus 2, 3-dimercaptopropanol. J. Biol. Chem. 193, 265 – 275.en_US
dc.identifier.citedreferenceSiegel G. J. and Fogt S. K. ( 1976 ) Lead ion activates phosphorylation of electroplax (Na +, K + )-ATPase in the absence of sodium ion. Arch. Biochem. Biophys. 174, 744 – 746.en_US
dc.identifier.citedreferenceSkou J-C. ( 1975 ) The (Na +, K + ) activated enzyme system and its relationship to transport of sodium and potassium. Quart. Rev. Biophys. 7, 401 – 434.en_US
dc.identifier.citedreferenceStahl W. L. and Broderson S. H. ( 1976a ) Histochemical localization of potassium stimulated p -nitrophenyl phosphatase activity in somatosensory cortex of the rat. J. Histochem. Cytochem. 24, 731 – 739.en_US
dc.identifier.citedreferenceStahl W. L. and Broderson S. H. ( 1976b ) Localization of (Na +, K + )ATPase in the brain. Fed. Proc. 35, 1260 – 1265.en_US
dc.identifier.citedreferenceSternberger L. A. ( 1976 ) Immunocytochemistry, Prentice-Hall, Englewood Cliffs, New Jersey.en_US
dc.identifier.citedreferenceSweadner K. J. ( 1978 ) Purification from brain of an intrinsic membrane protein fraction enriched in (Na + + K + )-ATPase. Biochem. Biophys. Acta 508, 486 – 499.en_US
dc.identifier.citedreferenceUesugi S., Dulak N. C., Dixon J. F., Hexum T. D., Dahl J. L., Perdue J. F. and Hokin L. E. ( 1969 ) Studies on the characterization of the sodium potassium transport adenosine triphosphatase. IV. Large scale purification and properties of a lubrol solubilized bovine brain enzyme. J. Biol. Chem. 25, 531 – 543.en_US
dc.identifier.citedreferenceWood J. B., Jean D. H., Whitaker J. N., McLaughlin N. J. and Albers R. W. ( 1977 ) Immunocytochemical localization of sodium potassium ATPase in knifefish brain. J. Neurocytol. 6, 571 – 581.en_US
dc.owningcollnameInterdisciplinary and Peer-Reviewed


Files in this item

Show simple item record

Remediation of Harmful Language

The University of Michigan Library aims to describe library materials in a way that respects the people and communities who create, use, and are represented in our collections. Report harmful or offensive language in catalog records, finding aids, or elsewhere in our collections anonymously through our metadata feedback form. More information at Remediation of Harmful Language.

Accessibility

If you are unable to use this file in its current format, please select the Contact Us link and we can modify it to make it more accessible to you.