Intracerebral NMDA Injection Stimulates Production of Interleukin-1Β in Perinatal Rat Brain
dc.contributor.author | Hagan, P. | en_US |
dc.contributor.author | Poole, S. | en_US |
dc.contributor.author | Bristow, A. F. | en_US |
dc.contributor.author | Tilders, F. | en_US |
dc.date.accessioned | 2010-04-01T14:54:37Z | |
dc.date.available | 2010-04-01T14:54:37Z | |
dc.date.issued | 1996-11 | en_US |
dc.identifier.citation | Hagan, P.; Poole, S.; Bristow, A. F.; Tilders, F. (1996). "Intracerebral NMDA Injection Stimulates Production of Interleukin-1Β in Perinatal Rat Brain." Journal of Neurochemistry 67(5): 2215-2218. <http://hdl.handle.net/2027.42/65366> | en_US |
dc.identifier.issn | 0022-3042 | en_US |
dc.identifier.issn | 1471-4159 | en_US |
dc.identifier.uri | https://hdl.handle.net/2027.42/65366 | |
dc.identifier.uri | http://www.ncbi.nlm.nih.gov/sites/entrez?cmd=retrieve&db=pubmed&list_uids=8863535&dopt=citation | en_US |
dc.description.abstract | Susceptibility to NMDA neurotoxicity peaks in the early postnatal period in rats. Although indirect evidence suggests that interleukin-1Β is a mediator of NMDA neurotoxicity in perinatal rats, direct confirmation of NMDA-induced interleukin-1Β production in the brain has not been reported previously. The primary goal of this study was to determine if intracerebral injection of a neurotoxic dose of NMDA stimulates interleukin-1Β production acutely. We used a rat-specific interleukin-1Β ELISA to quantify brain tissue homogenate interleukin-1Β content, and an immunocytochemical assay with a monoclonal anti-rat interleukin-1Β antibody to visualize its distribution. NMDA (10 nmol) was injected stereotaxically into 7-day-old rats, using coordinates that targeted the striatum and overlying dorsal hippocampus. Interleukin-1Β concentrations were measured in samples from the injected and contralateral cerebral hemispheres 0–12 h later; in addition, the impact of treatment with the noncompetitive NMDA antagonist MK-801 on interleukin-1Β production was assessed. We found marked increases in tissue content of interleukin-1Β in the lesioned hemisphere; values peaked at 6 h post injection. Treatment with MK-801 (1 mg/kg) blocked NMDA-induced increases in interleukin-1Β. Preliminary immunocytochemical analysis demonstrated high concentrations of interleukin-1Β-immunoreactive cells in the lesioned hippocampus, and concurrent increases in interleukin-1Β immunoreactivity diffusely in the ependyma at 6 h after NMDA administration. Our data provide the first direct evidence that NMDA-induced excitotoxic injury stimulates interleukin-1Β production in vivo. | en_US |
dc.format.extent | 585096 bytes | |
dc.format.extent | 3110 bytes | |
dc.format.mimetype | application/pdf | |
dc.format.mimetype | text/plain | |
dc.publisher | Blackwell Science Ltd | en_US |
dc.rights | Blackwell Science Inc | en_US |
dc.subject.other | Interleukin-1Β | en_US |
dc.subject.other | N -Methyl-d-Aspartate | en_US |
dc.subject.other | MK-801 | en_US |
dc.subject.other | Rat Brain | en_US |
dc.title | Intracerebral NMDA Injection Stimulates Production of Interleukin-1Β in Perinatal Rat Brain | en_US |
dc.type | Article | en_US |
dc.rights.robots | IndexNoFollow | en_US |
dc.subject.hlbsecondlevel | Neurosciences | en_US |
dc.subject.hlbtoplevel | Health Sciences | en_US |
dc.description.peerreviewed | Peer Reviewed | en_US |
dc.contributor.affiliationother | † Department of Pharmacology, Free University of Amsterdam, Amsterdam, The Netherlands | en_US |
dc.identifier.pmid | 8863535 | en_US |
dc.description.bitstreamurl | http://deepblue.lib.umich.edu/bitstream/2027.42/65366/1/j.1471-4159.1996.67052215.x.pdf | |
dc.identifier.doi | 10.1046/j.1471-4159.1996.67052215.x | en_US |
dc.identifier.source | Journal of Neurochemistry | en_US |
dc.owningcollname | Interdisciplinary and Peer-Reviewed |
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