Uridine Metabolism in the Goldfish Retina During Optic Nerve Regeneration: Cell-Free Preparations
dc.contributor.author | Kohsaka, Shinichi | en_US |
dc.contributor.author | Dokas, Linda A. | en_US |
dc.contributor.author | Agranoff, Bernard W. | en_US |
dc.date.accessioned | 2010-04-01T15:02:32Z | |
dc.date.available | 2010-04-01T15:02:32Z | |
dc.date.issued | 1981-03 | en_US |
dc.identifier.citation | Kohsaka, Shinichi; Dokas, Linda A.; Agranoff, Bernard W. (1981). "Uridine Metabolism in the Goldfish Retina During Optic Nerve Regeneration: Cell-Free Preparations." Journal of Neurochemistry 36(3): 1166-1174. <http://hdl.handle.net/2027.42/65504> | en_US |
dc.identifier.issn | 0022-3042 | en_US |
dc.identifier.issn | 1471-4159 | en_US |
dc.identifier.uri | https://hdl.handle.net/2027.42/65504 | |
dc.identifier.uri | http://www.ncbi.nlm.nih.gov/sites/entrez?cmd=retrieve&db=pubmed&list_uids=6259282&dopt=citation | en_US |
dc.description.abstract | The activities of uridine kinase (EC 2.7.1.48), uridine monophosphate (UMP) kinase (EC 2.7.1.3.14), and uridine diphosphate (UDP) kinase (EC 2.7.4.6) were measured in retinal high-speed supernatant fractions following unilateral optic nerve crush in the goldfish. The enzyme activities followed a similar time course, with initial increases 2-3 days following nerve crush, peak activity at 4 days, and a gradual return to basal levels by day 21. The magnitude of the stimulation on day 4 was about 35% in each case. Activities of two enzymes of intermediary metabolism, pyruvate kinase (EC 2.7.1.40) and lactic dehydrogenase (EC 1.1.1.27), were not altered, indicating that the coordinate increases in nucleoside and nucleotide kinase activities were specific responses to the nerve injury. The increased labeling could not be explained by altered phosphohydrolytic activities. The nature of the enhancement was further studied in UDP kinase, the most active of the kinases examined. Neither low-molecular-weight components nor substrate availability could account for the observed increase in UDP kinase in the 4 day post-crush retinas. The K m , for UDP was unaltered, and a mixing experiment did not support the possibility that stimulatory or inhibitory factors played a role. The enhancement of UDP kinase activity was blocked by injection of actinomycin D following nerve crush. The results suggest that the observed increases in enzymes of uridine metabolism result from their increased formation following nerve crush. | en_US |
dc.format.extent | 801063 bytes | |
dc.format.extent | 3110 bytes | |
dc.format.mimetype | application/pdf | |
dc.format.mimetype | text/plain | |
dc.publisher | Blackwell Publishing Ltd | en_US |
dc.rights | 1981 International Society for Neurochemistry Ltd. | en_US |
dc.subject.other | Uridine | en_US |
dc.subject.other | Goldfish Retina | en_US |
dc.subject.other | Regeneration | en_US |
dc.subject.other | Nucleoside Kinase | en_US |
dc.subject.other | Nucleotide Kinase | en_US |
dc.title | Uridine Metabolism in the Goldfish Retina During Optic Nerve Regeneration: Cell-Free Preparations | en_US |
dc.type | Article | en_US |
dc.rights.robots | IndexNoFollow | en_US |
dc.subject.hlbsecondlevel | Neurosciences | en_US |
dc.subject.hlbtoplevel | Health Sciences | en_US |
dc.description.peerreviewed | Peer Reviewed | en_US |
dc.contributor.affiliationum | Neuroscience Laboratory, Mental Health Research Institute and Department of Biological Chemistry, The University of Michigan, Ann Arbor, Michigan, U.S.A. | en_US |
dc.identifier.pmid | 6259282 | en_US |
dc.description.bitstreamurl | http://deepblue.lib.umich.edu/bitstream/2027.42/65504/1/j.1471-4159.1981.tb01714.x.pdf | |
dc.identifier.doi | 10.1111/j.1471-4159.1981.tb01714.x | en_US |
dc.identifier.source | Journal of Neurochemistry | en_US |
dc.identifier.citedreference | Agarwal R. P. and Parks, R. E. Jr. ( 1971 ) Erythrocytic nucleoside diphosphokinase. J. Biol. Chem. 246, 2258 – 2264. | en_US |
dc.identifier.citedreference | Agranoff B. W., Smith C. B., and Sokoloff L. ( 1980 ) Regional protein synthesis in rat brain after hypoglossal axotomy. Trans. Am. Soc. Neurochem. 11 ( 1 ), 95. | en_US |
dc.identifier.citedreference | Anderson E. P. ( 1973 ) Nucleoside and nucleotide kinases. in The Enzymes, 3rd ed. ( Boyer, P. D., ed ), pp. 49 – 96. Academic Press, New York. | en_US |
dc.identifier.citedreference | Bandurski R. S. and Axelrod B. ( 1951 ) The chromatographic identification of some biologically important phosphate esters. J. Biol. Chem. 193, 405 – 410. | en_US |
dc.identifier.citedreference | Burrell H. R., Dokas L. A., and Agranoff B. W. ( 1978 ) RNA metabolism in the goldfish retina during optic nerve regeneration. J. Neurochem. 31, 289 – 298. | en_US |
dc.identifier.citedreference | Burrell H. R., Heacock A. M., Water R. D., and Agranoff B. W. ( 1979 ) Increased tubulin messenger RNA in the goldfish retina during optic nerve regeneration. Brain Res. 168, 628 – 632. | en_US |
dc.identifier.citedreference | Cotman C. W. and Nadler J. V. ( 1978 ) Reactive synaptogenesis in the hippocampus. in Neuronal Plasticity ( Cotman, C. W., ed ), pp. 227 – 271. Raven Press, New York. | en_US |
dc.identifier.citedreference | Cragg B. G. ( 1970 ) What is the signal for chromatolysis? Brain Res. 23, 1 – 21. | en_US |
dc.identifier.citedreference | Dokas L. A. and Agranoff B. W. ( 1979 ) RNA precursor metabolism characterized during optic nerve regeneration. Trans. Am. Soc. Neurochem. 10 ( 1 ), 232. | en_US |
dc.identifier.citedreference | Dokas L. A., Kohsaka S., Burrell H. R., and Agranoff B. W. ( 1981 ) Uridine metabolism in the goldfish retina during optic nerve regeneration: Whole retina studies. J. Neurochem. 36, 1160 – 1165. | en_US |
dc.identifier.citedreference | Dunlop D. S., Van Elder W., and Lajtha A. ( 1974 ) Measurement of rates of protein synthesis in rat brain slices. J. Neurochem. 22, 821 – 830. | en_US |
dc.identifier.citedreference | Giulian D., Ruisseaux H. D., and Cowburn D. ( 1980 ) Biosynthesis and intra-axonal transport of proteins during neuronal regeneration. J. Biol. Chem. 255, 6494 – 6501. | en_US |
dc.identifier.citedreference | Goldenberg G. J. and Stein W. D. ( 1978 ) Stimulation of uridine uptake in 3T3 cells is associated with increased ATP affhity of uridine phosphorylating system. Nature 274, 475 – 477. | en_US |
dc.identifier.citedreference | Grafstein B. and McQuarrie I. G. ( 1978 ) Role of the nerve cell body in axonal regeneration, in Neuronal Plasticity ( Cotman, C. W., ed ), pp. 155 – 195. Raven Piess, New York. | en_US |
dc.identifier.citedreference | Heacock A. M. and Agranoff B. W. ( 1976 ) Enchanced labeling of a retinal protein during regeneration of optic nerve in goldfish. Proc. Natl. Acad. Sci. USA 73, 828 – 832. | en_US |
dc.identifier.citedreference | Ingoglia N. A. ( 1978 ) The effect of intraocular injection of cordycepin on retinal RNA synthesis and on RNA axonally transported during regeneration of the optic nerves of goldfish. J. Neurochem. 30, 1029 – 1039. | en_US |
dc.identifier.citedreference | Kaye P. L., Jeffrey R. L., and Austin L. ( 1978 ) Bilateral enzymic changes in rat nodose ganglia following unilateral cervical vagotomy. Neuroscience 3, 1231 – 1239. | en_US |
dc.identifier.citedreference | Kohsaka S., Schwartz M., and Agranoff S. W. ( 1981 ) Increased activity of ornithine decarboxylase in goldfish following optic nerve crush. Dev. Brain Res. ( in press ). | en_US |
dc.identifier.citedreference | Krystal G. and Scholefield P. G. ( 1973 ) The partial purification and properties of uridine kinase from Ehrlich ascites tumor cells. Can. J. Biochem. 51, 379 – 389. | en_US |
dc.identifier.citedreference | Levine R. L., Hogenraad N. J., and Kretchmer N. ( 1974 ) A review: Biological and clinical aspects of pyrimidine metabolism. Pediatr. Res. 8, 724 – 734. | en_US |
dc.identifier.citedreference | Lowry O. H., Rosebrough N. J., Farr A. L., and Randall R. J. ( 1951 ) Protein measurement with the Folin phenol reagent. J. Biol. Chem. 193, 265 – 275. | en_US |
dc.identifier.citedreference | Lynch G. and Cotman C. W. ( 1975 ) The hippocampus as a model for studying anatomical plasticity in the adult brain, in The Hippocampus Vol. I: Structure and Development ( Isaacson, R. L., and Pribam, K. H., eds ), pp. 123 – 154. Plenum Press, New York. | en_US |
dc.identifier.citedreference | MacNeal R. K. and Punch D. L. ( 1978 ) Stoichiometry and role of GTP hydrolysis in bovine neurotubule assembly. J. Biol. Chem. 253, 4683 – 4687. | en_US |
dc.identifier.citedreference | Mandel P. ( 1971 ) Free nucleotides, in Handbook of Neurochemistry ( Lajtha, A., ed ), pp. 249 – 281. Plenum Press, New York. | en_US |
dc.identifier.citedreference | Martin T. J. F. and Tashjian A. H. ( 1978 ) Thyrotropin-releasing hormone modulation of uridine uptake in rat pituitary cells. Evidence that uridine phosphorylation is regulated. J. Biol. Chem. 253, 106 – 115. | en_US |
dc.identifier.citedreference | Murray M. ( 1973 ) 3 H-Uridine incorporation by regenerating retinal ganglion cells of goldfish. Exp. Neurol. 39, 489 – 497. | en_US |
dc.identifier.citedreference | Murray M. ( 1976 ) Regeneration of retinal axons into the goldfish optic tectum. J. Comp. Neurol. 168, 175 – 196. | en_US |
dc.identifier.citedreference | Murray M. and Grafstein B. ( 1969 ) Change in the morphology and amino acid incorporation of regenerating goldfish optic neurons. Exp. Neurol. 23, 544 – 560. | en_US |
dc.identifier.citedreference | Nicholas P. C. and Bachelard H. S. ( 1974 ) Kinetic properties of cerebral pyruvate kinase. Biochem. J. 141, 165 – 171. | en_US |
dc.identifier.citedreference | Nickerson J. A. and Wells W. W. ( 1978 ) Association of nucleoside diphosphate kinase with microtubules. Biochem. Biophys. Res. Commun. 85, 820 – 826. | en_US |
dc.identifier.citedreference | Singer P. A. and Mahler S. ( 1979 ) The time course of increased glucose utilization in hypoglossal nucleus neurons during regeneration. Soc. Neurosci. 5, 683 ( Abstr. ). | en_US |
dc.identifier.citedreference | Springer A. D. and Agranoff B. W. ( 1977 ) Effect of temperature on rate of goldfish optic nerve regeneration: A radioautographic and behavioral study. Brain Res. 128, 405 – 415. | en_US |
dc.identifier.citedreference | Terry B. J. and Punch D. L. ( 1979 ) Nucleotide release from tubulin and nucleoside 5′-diphosphate kinase action in microtubule assembly. J. Biol. Chem. 254, 9469 – 9476. | en_US |
dc.identifier.citedreference | Valentin-Hansen P. ( 1978 ) Uridine-cytidine kinase from Escherichia coli, in Methods in Enzymology, Vol. LI ( Hoffee, P. A. and Jones, M. E., eds ), pp. 308 – 314. Academic Press, New York. | en_US |
dc.identifier.citedreference | Watanabe T. and Flavin M. ( 1976 ) Nucleotide-metabolizing enzymes in chlamydonas flagella. J. Biol. Chem. 251, 182 – 192. | en_US |
dc.identifier.citedreference | Wroblewski F. and LaDue J. S. ( 1955 ) Lactic dehydrogenase activity in blood. Proc. Soc. Exp. Biol. Med. 90, 210 – 213. | en_US |
dc.owningcollname | Interdisciplinary and Peer-Reviewed |
Files in this item
Remediation of Harmful Language
The University of Michigan Library aims to describe library materials in a way that respects the people and communities who create, use, and are represented in our collections. Report harmful or offensive language in catalog records, finding aids, or elsewhere in our collections anonymously through our metadata feedback form. More information at Remediation of Harmful Language.
Accessibility
If you are unable to use this file in its current format, please select the Contact Us link and we can modify it to make it more accessible to you.