View Item 

Show simple item record

Arachidonic Acid Release and Catecholamine Secretion from Digitonin-Treated Chromaffin Cells: Effects of Micromolar Calcium, Phorbol Ester, and Protein Alkylating Agents
dc.contributor.authorFrye, Roy A.en_US
dc.contributor.authorHolz, Ronald W.en_US
dc.date.accessioned2010-04-01T15:04:17Z
dc.date.available2010-04-01T15:04:17Z
dc.date.issued1985-01en_US
dc.identifier.citationFrye, Roy A.; Holz, Ronald W. (1985). "Arachidonic Acid Release and Catecholamine Secretion from Digitonin-Treated Chromaffin Cells: Effects of Micromolar Calcium, Phorbol Ester, and Protein Alkylating Agents." Journal of Neurochemistry 44(1): 265-273. <http://hdl.handle.net/2027.42/65534>en_US
dc.identifier.issn0022-3042en_US
dc.identifier.issn1471-4159en_US
dc.identifier.urihttps://hdl.handle.net/2027.42/65534
dc.identifier.urihttp://www.ncbi.nlm.nih.gov/sites/entrez?cmd=retrieve&db=pubmed&list_uids=3917291&dopt=citationen_US
dc.description.abstractThe relationship between catecholamine secretion and arachidonic acid release from digitonin-treated chromaffin cells was investigated. Digitonin renders permeable the plasma membranes of bovine adrenal chromaffin cells to Ca 2+ , ATP, and proteins. Digitonin-treated cells undergo exocytosis of catecholamine in response to micromolar Ca 2+ in the medium. The addition of micromolar Ca 2+ to digitonin-treated chromaffin cells that had been prelabeled with [ 3 H]arachidonic acid caused a marked increase in the release of [ 3 H]arachidonic acid. The time course of [ 3 H]arachidonic acid release paralleled catecholamine secretion. Although [ 3 H]arachidonic acid release and exocytosis were both activated by free Ca 2+ in the micromolar range, the activation of [ 3 H]arachidonic acid release occurred at Ca 2+ concentrations slightly lower than those required to activate exocytosis. Pretreatment of the chromaffin cells with N -ethylmaleimide (NEM) or p -bromophenacyl bromide (BPB) resulted in dose-dependent inhibition of 10 Μ M Ca 2+ -stimulated [ 3 H]arachidonic acid release and exocytosis. The IC 50 of NEM for both [ 3 H]arachidonic acid release and exocytosis was 40 Μ M. The IC 50 of BPB for both events was 25 Μ M. High concentrations (5–20 m M ) of Mg 2+ caused inhibition of catecholamine secretion without altering [ 3 H]arachidonic acid release. A phorbol ester that activates protein kinase C, 12- O -tetradecanoylphorbol-13-acetate (TPA), caused enhancement of both [ 3 H]arachidonic acid release and exocytosis. The findings demonstrate that [ 3 H]arachidonic acid release is stimulated during catecholamine secretion from digitonin-treated chromaffin cells and they are consistent with a role for phospholipase A 2 in exocytosis from chromaffin cells. Furthermore the data suggest that protein kinase C can modulate both arachidonic acid release and exocytosis.en_US
dc.format.extent877393 bytes
dc.format.extent3110 bytes
dc.format.mimetypeapplication/pdf
dc.format.mimetypetext/plain
dc.publisherBlackwell Publishing Ltden_US
dc.rights1985 International Society for Neurochemistryen_US
dc.subject.otherCatecholamine Secretionen_US
dc.subject.otherArachidonic Acid Releaseen_US
dc.subject.otherChromaffin Cellsen_US
dc.subject.otherDigitoninen_US
dc.titleArachidonic Acid Release and Catecholamine Secretion from Digitonin-Treated Chromaffin Cells: Effects of Micromolar Calcium, Phorbol Ester, and Protein Alkylating Agentsen_US
dc.typeArticleen_US
dc.rights.robotsIndexNoFollowen_US
dc.subject.hlbsecondlevelNeurosciencesen_US
dc.subject.hlbtoplevelHealth Sciencesen_US
dc.description.peerreviewedPeer Revieweden_US
dc.contributor.affiliationumDepartment of Pharmacology, The University of Michigan Medical School, Ann Arbor, Michigan, U.S.A.en_US
dc.identifier.pmid3917291en_US
dc.description.bitstreamurlhttp://deepblue.lib.umich.edu/bitstream/2027.42/65534/1/j.1471-4159.1985.tb07140.x.pdf
dc.identifier.doi10.1111/j.1471-4159.1985.tb07140.xen_US
dc.identifier.sourceJournal of Neurochemistryen_US
dc.identifier.citedreferenceAhkong Q. F., Cramp F. C., Fisher D., Howell J. I., and Lucy J. A. ( 1972 ) Studies on chemically induced cell fusion. J. Cell Sci. 10, 769 – 787.en_US
dc.identifier.citedreferenceBell R. L., Kennerly D. A., Stanford N., and Majerus P. W. ( 1979 ) Diglyceride lipase: a pathway for arachidonate release from human platelets. Proc. Natl. Acad. Sci. USA 76, 3238 – 3241.en_US
dc.identifier.citedreferenceBlackwell G. J., Carnuccio R., DiRosa M., Flower R. J., Langham C. S. J., Parente L., Persico P., Russell-Smith N. C., and Stone D. ( 1982 ) Glucocorticoids induce the formation and release of anti-inflammatory and anti-phospholipase proteins into the peritoneal cavity of the rat. Br. J. Pharmacol. 76, 185 – 194.en_US
dc.identifier.citedreferenceBills T. K., Smith J. B., and Silver M. J. ( 1976 ) Metabolism of [ 14 C]arachidonic acid by human platelets. Biochim. Biophys. Acta 424, 303 – 314.en_US
dc.identifier.citedreferenceCastagna M., Takai Y., Kaibuchi K., Sano K., Kikkawa U., and Nishizuka Y. ( 1982 ) Direct activation of calcium-activated, phospholipid-dependent protein kinase by tumor promoting phorbol esters. J. Biol. Chem. 257, 7847 – 7851.en_US
dc.identifier.citedreferenceCreutz C. E. ( 1981 ) cis -Unsaturated fatty acids induce the fusion of chromaffin granules aggregated by synexin. J. Cell. Biol. 91, 247 – 256.en_US
dc.identifier.citedreferenceDonnelly T. E. and Jensen R. ( 1983 ) Effect of fluphenazine on the stimulation of calcium-sensitive phospholipid-dependent protein kinase by 12- O -tetradecanoyl phorbol-13-acetate. Life Sci. 33, 2247 – 2253.en_US
dc.identifier.citedreferenceDunn L. A. and Holz R. W. ( 1983 ) Catecholamine secretion from digitonin-treated adrenal medullary chromaffin cells. J. Biol. Chem. 258, 4989 – 4993.en_US
dc.identifier.citedreferenceFenwick E. M., Fajdiga P. B., Howe N. S. B., and Livett B. G. ( 1978 ) Functional and morphological characterization of isolated bovine adrenal medullary cells. J. Cell. Biol. 76, 12 – 30.en_US
dc.identifier.citedreferenceFrye R. A. and Holz R. W. ( 1983 ) Phospholipase A 2 inhibitors block catecholamine secretion and calcium uptake in cultured bovine adrenal medullary cells. Mol. Pharmacol. 23, 547 – 550.en_US
dc.identifier.citedreferenceFrye R. A. and Holz R. W. ( 1984 ) The relationship between arachidonic acid release and catecholamine secretion from cultured bovine adrenal chromaffin cells. J. Neurochem. 43, 146 – 150.en_US
dc.identifier.citedreferenceHirata F. ( 1981 ) The regulation of lipomodulin, a phospholipase inhibitory protein in rabbit neutrophils by phosphorylation. J. Biol. Chem. 256, 7730 – 7733.en_US
dc.identifier.citedreferenceHirata F., Schiffmann E., Venkatasubramanian K., Solomon D., and Axelrod J. ( 1980 ) A phospholipase A 2 inhibitory protein in rabbit neutrophils induced by glucocorticoids. Proc. Natl. Acad. Sci. USA 77, 2533 – 2536.en_US
dc.identifier.citedreferenceHoffman S. L., Prescott S. M., and Majerus P. W. ( 1982 ) The effects of mepacrine and p-bromophenacyl bromide on arachidonic acid release in human platelets. Arch. Biochem. Biophys. 215, 237 – 244.en_US
dc.identifier.citedreferenceKilpatrick D. L., Ledbetter F. H., Carson K. A., Kirshner A. G., Slepetis R., and Kirshner N. ( 1980 ) Stability of bovine adrenal medulla cells in culture. J. Neurochem. 35, 679 – 692.en_US
dc.identifier.citedreferenceKikkawa U., Takai Y., Tanaka Y., Miyake R., and Nishizuka Y. ( 1983 ) Protein kinase C as a possible receptor protein of tumor promoting phorbol esters. J. Biol. Chem. 258, 11442 – 11445.en_US
dc.identifier.citedreferenceKnight D. E. and Baker P. F. ( 1983 ) The phobol ester TPA increases the affinity of exocytosis for calcium in ‘leaky’ adrenal medullary cells. FEBS Lett. 160, 98 – 100.en_US
dc.identifier.citedreferenceMarone G., Kagey-Sobotka A., and Lichtenstein L. M. ( 1981 ) Possible role of phospholipase A 2 in triggering histamine secretion from human basophils in vitro. Clin. Immunol. Immunopathol. 20, 231 – 239.en_US
dc.identifier.citedreferenceMcGivney A., Morita Y., Crews F. T., Hirata F., Axelrod J., and Siraganian R. P. ( 1981 ) Phospholipase activation in the IgE-mediated and Ca 2+ ionophore A23187-induced release of histamine from rat basophilic leukemia cells. Arch. Biochem. Biophys. 212, 572 – 580.en_US
dc.identifier.citedreferenceMufson R. A., DeFeo D., and Weinstein I. B. ( 1979 ) Effects of phorbol ester tumor promoters on arachidonic acid metabolism in chick embryo fibroblasts. Mol. Pharmacol. 16, 569 – 578.en_US
dc.identifier.citedreferenceNaor Z. and Catt K. J. ( 1981 ) Mechanism of action of gonadotropin releasing hormone. Involvement of phospholipid turnover in luteinizing hormone release. J. Biol. Chem. 256, 2226 – 2229.en_US
dc.identifier.citedreferenceNiedel J. E., Kuhn L. J., and Vandenbark G. R. ( 1983 ) Phorbol diester receptor copurifies with protein kinase C. Proc. Natl. Acad. Sci. USA 80, 36 – 40.en_US
dc.identifier.citedreferencehuchi K. and Levine L. ( 1978 ) Stimulation of prostaglandin synthesis by tumor-promoting phorbol-12,13-diesters in canine kidney (MDCK) cells. J. Biol. Chem. 253, 4783 – 4790.en_US
dc.identifier.citedreferencePortzehl H., Caldwell P. C., and Ruegg J. C. ( 1964 ) The dependence of contraction and relaxation of muscle fibres from the crab Maia squinado on the internal concentration of free calcium ions. Biochim. Biophys. Acta 79, 581 – 591.en_US
dc.identifier.citedreferenceReddy T. S. and Bazan N. G. ( 1983 ) Kinetic properties of arachidonoyl-coenzyme A synthetase in rat brain microsomes. Arch. Biochem. Biophys. 226, 125 – 133.en_US
dc.identifier.citedreferenceRittenhouse-Simmons S. ( 1979 ) Production of diglyceride from phosphatidyl inositol in activated human platelets. J. Clin. Invest. 63, 580 – 587.en_US
dc.identifier.citedreferenceRoberts L. J., Lewis R. A., Oates J. A., and Austen K. F. ( 1979 ) Prostaglandin, thromboxane, and 12-hydroxy-5,8,10,14-eicosatetraenoic acid production by ionophore-stimulated rat serousal mast cells. Biochim. Biophys. Acta 575, 185 – 192.en_US
dc.identifier.citedreferenceSilk S. T., Wong K. T. H., and Marcus A. J. ( 1981 ) Arachidonic acid releasing activity in platelet membranes: effects of sulfhydryl-modifying reagents. Biochemistry 20, 391 – 397.en_US
dc.identifier.citedreferenceSmith A. D. and Winkler H. ( 1968 ) Lysosomal phospholipases A 1 and A 2 of bovine adrenal medulla. Biochem. J. 108, 867 – 874.en_US
dc.identifier.citedreferenceSmolen J. E. and Weissman G. ( 1980 ) Effects of indomethacin, 5,8,11,14-eicosatetraynoic acid, and p -bromophenacyl bromide on lysosomal enzyme release and superoxide anion generation by human polymorphonuclear leukocytes. Biochem. Pharmacol. 29, 533 – 538.en_US
dc.identifier.citedreferenceViveros O. H. ( 1975 ) Mechanism of secretion of catecholamines from adrenal medulla, in Handbook of Physiology Endocrinology, Sec. 7, Vol. 6, ( Blaschko H., Sayers G., and Smith A. D., eds ), pp. 389 – 426. American Physiological Society. Washington, D.C.en_US
dc.identifier.citedreferenceVolverk J. J., Pieterson W. A., and de Haas G. H. ( 1974 ) Histidine at the active site of phospholipase A 2. Biochemistry 13, 1446 – 1454.en_US
dc.identifier.citedreferenceWalenga R. W., Opas E. E., and Feinstein M. B. ( 1981 ) Differential effects of calmodulin antagonists on phospholipase A 2 and C in thrombin-stimulated platelets. J. Biol. Chem. 256, 12523 – 12528.en_US
dc.identifier.citedreferenceWaymire J. C., Bennett W. F., Boehme R., Hanteins L., Gilmer-Waymire K., and Haycock J. ( 1983 ) Bovine adrenal chromaffin cells: high yield purification and viability in suspension culture. J. Neurosci. Meth. 7, 329 – 351.en_US
dc.identifier.citedreferenceWightman P. D., Humes J. L., Davies P., and Bonney R. J. ( 1981 ) Identification and characterization of 2 phospholipase A 2 activities in resident mouse peritoneal macrophages. Biochem. J. 195, 427 – 433.en_US
dc.identifier.citedreferenceWightman P. D., Dahlgren M. E., and Bonney R. J. ( 1982 ) Protein kinase activation of phospholipase A 2 in sonicates of mouse peritoneal macrophages. J. Biol. Chem. 257, 6650 – 6652.en_US
dc.identifier.citedreferenceWilson S. P. and Kirshner N. ( 1983 ) Calcium-evoked secretion from digitonin-permeabilized adrenal medullary chromaffin cells. J. Biol. Chem. 258, 4994 – 5000.en_US
dc.identifier.citedreferenceWilson D. B., Prescott S. M., and Majerus P. W. ( 1982 ) Discovery of an arachidonyl coenzyme A synthetase in human platelets. J. Biol. Chem. 257, 3510 – 3515.en_US
dc.identifier.citedreferenceYamanishi J., Takai Y., Kaibuchi K., Sano K., Castagna M., and Nishizuka Y. ( 1983 ) Synergistic functions of phorbol ester and calcium in serotonin release from human platelets. Biochem. Biophys. Res. Commun. 112, 778 – 786.en_US
dc.owningcollnameInterdisciplinary and Peer-Reviewed


Files in this item

Name:
j.1471-4159.1985.tb07140.x.pdf
Size:
856.8KB
Format:
PDF
View/Open

Show simple item record

Remediation of Harmful Language

The University of Michigan Library aims to describe library materials in a way that respects the people and communities who create, use, and are represented in our collections. Report harmful or offensive language in catalog records, finding aids, or elsewhere in our collections anonymously through our metadata feedback form. More information at Remediation of Harmful Language.

Accessibility

If you are unable to use this file in its current format, please select the Contact Us link and we can modify it to make it more accessible to you.