Coupling Efficacy and Selectivity of the Human Μ-Opioid Receptor Expressed as Receptor—GΑ Fusion Proteins in Escherichia coli
dc.contributor.author | Stanasila, Laura | en_US |
dc.contributor.author | Lim, William K. | en_US |
dc.contributor.author | Neubig, Richard R. | en_US |
dc.contributor.author | Pattus, Franc | en_US |
dc.date.accessioned | 2010-04-01T15:09:41Z | |
dc.date.available | 2010-04-01T15:09:41Z | |
dc.date.issued | 2000-09 | en_US |
dc.identifier.citation | Stanasila, Laura; Lim, William K.; Neubig, Richard R.; Pattus, Franc (2000). "Coupling Efficacy and Selectivity of the Human Μ-Opioid Receptor Expressed as Receptor—GΑ Fusion Proteins in Escherichia coli ." Journal of Neurochemistry 75(3): 1190-1199. <http://hdl.handle.net/2027.42/65628> | en_US |
dc.identifier.issn | 0022-3042 | en_US |
dc.identifier.issn | 1471-4159 | en_US |
dc.identifier.uri | https://hdl.handle.net/2027.42/65628 | |
dc.identifier.uri | http://www.ncbi.nlm.nih.gov/sites/entrez?cmd=retrieve&db=pubmed&list_uids=10936202&dopt=citation | en_US |
dc.format.extent | 793073 bytes | |
dc.format.extent | 3110 bytes | |
dc.format.mimetype | application/pdf | |
dc.format.mimetype | text/plain | |
dc.publisher | Blackwell Science Ltd. | en_US |
dc.rights | International Society for Neurochemistry | en_US |
dc.subject.other | G Protein-coupled Receptors | en_US |
dc.subject.other | Receptor-G Protein Fusion | en_US |
dc.subject.other | Μ-Opioid Receptor | en_US |
dc.subject.other | Escherichia Coli | en_US |
dc.subject.other | Pharmacology | en_US |
dc.title | Coupling Efficacy and Selectivity of the Human Μ-Opioid Receptor Expressed as Receptor—GΑ Fusion Proteins in Escherichia coli | en_US |
dc.type | Article | en_US |
dc.rights.robots | IndexNoFollow | en_US |
dc.subject.hlbsecondlevel | Neurosciences | en_US |
dc.subject.hlbtoplevel | Health Sciences | en_US |
dc.description.peerreviewed | Peer Reviewed | en_US |
dc.contributor.affiliationum | Department of Pharmacology, University of Michigan, Ann Arbor, Michigan, U.S.A. | en_US |
dc.identifier.pmid | 10936202 | en_US |
dc.description.bitstreamurl | http://deepblue.lib.umich.edu/bitstream/2027.42/65628/1/j.1471-4159.2000.0751190.x.pdf | |
dc.identifier.doi | 10.1046/j.1471-4159.2000.0751190.x | en_US |
dc.identifier.source | Journal of Neurochemistry | en_US |
dc.identifier.citedreference | Alt A., Mansour A., Akil H., Medzihradski F., Traynor J.R., Woods J.H. ( 1998 ) Stimulation of guanosine-5′- O -(3-[ 35 S]thio) triphosphate binding by endogenous opioids acting at a cloned mu receptor. J. Pharmacol. Exp. Ther. 286 282 – 288. | en_US |
dc.identifier.citedreference | Befort K., Tabbara L., Kieffer B.L. ( 1996 ) [ 35 S]GTP gamma S binding: a tool to evaluate functional activity of a cloned opioid receptor transiently expressed in COS cells. Neurochem. Res. 21 1301 – 1307. | en_US |
dc.identifier.citedreference | Bertin B., Freissmuth M., Jockers R., Strosberg A.D., Marullo S. ( 1994 ) Cellular signaling by an agonist-activated receptor/Gs alpha fusion protein. Proc. Natl. Acad. Sci. USA 91 8827 – 8831. | en_US |
dc.identifier.citedreference | Blake A.D., Bot G., Freeman J.C., Reisine T. ( 1997 ) Differential opioid agonist regulation of the mouse mu opioid receptor. J. Biol. Chem. 272 782 – 790. | en_US |
dc.identifier.citedreference | Bradford M.M. ( 1976 ) A rapid and sensitive method for the quantitation of microgram quantities of protein using the principle of protein-dye binding. Anal. Biochem. 72 248 – 254. | en_US |
dc.identifier.citedreference | Burt A.R., Sautel M., Wilson M.A., Rees S., Wise A., Milligan G. ( 1998 ) Agonist occupation of an alpha2A-adrenoreceptor-Gi 1 alpha fusion protein results in activation of both receptor-linked and endogenous Gi proteins. Comparisons of their contributions to GTPase activity and signal transduction and analysis of receptor-G protein activation stoichiometry. J. Biol. Chem. 273 10367 – 10375. | en_US |
dc.identifier.citedreference | Chakrabarti S., Prather P.L., Yu L., Law P., Loh H.H. ( 1995 ) Expression of the Μ-opioid receptor in CHO cells: ability of Μ-opioid ligands to promote Α-azidoanilido[ 32 P]GTP labeling of multiple G protein Α subunits. J. Neurochem. 64 2534 – 2543. | en_US |
dc.identifier.citedreference | Chakrabarti S., Law P., Loh H.H. ( 1998 ) Distinct differences between morphine- and [D-Ala 2, N -MePhe 4, Gly-ol 5 ]-enkephalin-Μ-opioid receptor complexes demonstrated by cyclic AMP-dependent protein kinase phosphorylation, J. Neurochem. 71 231 – 239. | en_US |
dc.identifier.citedreference | Chan J.S.C., Chiu T.T., Wong Y.H. ( 1995 ) Activation of type II adenylyl cyclase by the cloned Μ-opioid receptor: coupling to multiple G proteins. J. Neurochem. 65 2682 – 2689. | en_US |
dc.identifier.citedreference | Childers S.R. ( 1993 ) Opioid receptor-coupled second messenger systems, in Opioids I, Vol. 104, Part I (Herz A., ed), pp. 189 – 208. Springer-Verlag, Berlin. | en_US |
dc.identifier.citedreference | Fan L., Gioannini T.L., Wolinsky T., Hiller J.M., Simon E.J. ( 1995 ) Functional reconstitution of a highly purified Μ-opioid receptor protein with purified G proteins in liposomes. J. Neurochem. 65 2537 – 2542. | en_US |
dc.identifier.citedreference | Ferguson K., Higashijima T., Smigel M.D., Gilman A.G. ( 1986 ) The influence of bound GDP on the kinetics of guanine nucleotide binding to G proteins. J. Biol. Chem. 261 7393 – 7399. | en_US |
dc.identifier.citedreference | Gaibelet G., Meilhoc E., Riond J., Saves I., Exner T., Liaubet L., Nurnberg B., Masson J., Emorine L.J. ( 1999 ) Nonselective coupling of the human Μ-opioid receptor to multiple inhibitory G-protein isoforms. Eur. J. Biochem. 261 517 – 523. | en_US |
dc.identifier.citedreference | Jockers R., Linder M.E., Hohenegger M., Nanoff C., Bertin B., Strosberg A.D., Marullo S., Freissmuth M. ( 1994 ) Species difference in the G protein selectivity of the human and bovine A1-adenosine receptor. J. Biol. Chem. 269 32077 – 32084. | en_US |
dc.identifier.citedreference | Jones D.T. & Reed R.R. ( 1987 ) Molecular cloning of five GTP-binding protein cDNA species from rat olfactory neuroepithelium. J. Biol. Chem. 262 14241 – 14249. | en_US |
dc.identifier.citedreference | Kieffer B.L. ( 1995 ) Recent advances in molecular recognition and signal transduction of active peptides: receptors for opioid peptides. Cell Mol. Neurobiol. 15 615 – 635. | en_US |
dc.identifier.citedreference | Laugwitz K.L., Offermanns S., Spicher K., Schultz G. ( 1993 ) Mu and delta opioid receptors differentially couple to G protein subtypes in membranes of human neuroblastoma SH-SY5Y cells. Neuron 10 233 – 242. | en_US |
dc.identifier.citedreference | Law S.F. & Reisine T. ( 1997 ) Changes in association of G protein subunits with the cloned mouse delta opioid receptor on agonist stimulation. J. Pharmacol. Exp. Ther. 281 1476 – 1486. | en_US |
dc.identifier.citedreference | Leff P., Scaramellini C., Law C., McKechnie K. ( 1997 ) A three-state receptor model of agonist action. Trends Pharmacol. Sci. 18 355 – 362. | en_US |
dc.identifier.citedreference | Mestek A., Hurley J.H., Bye L.S., Campbell A.D., Chen Y., Tian M., Liu J., Schulman H., Yu L. ( 1995 ) The human mu opioid receptor: modulation of functional desensitization by calcium/calmodulin-dependent protein kinase and protein kinase C. J. Neurosci. 15 2396 – 2406. | en_US |
dc.identifier.citedreference | Milligan G. ( 2000 ) Insights into ligand pharmacology using receptor-G-protein fusion proteins. Trends Pharmacol. Sci. 21 24 – 28. | en_US |
dc.identifier.citedreference | Murthy K.S. & Makhlouf G.M. ( 1996 ) Opioid mu, delta, and kappa receptor-induced activation of phospholipase C-beta 3 and inhibition of adenylyl cyclase are mediated by Gi2 and Go in smooth muscle. Mol. Pharmacol. 50 870 – 877. | en_US |
dc.identifier.citedreference | North R.A. ( 1993 ) Opioid action on membrane ion channels, in Opioids I, Vol. 104, Part I (Herz A., ed), pp. 773 – 793. Springer-Verlag, Berlin. | en_US |
dc.identifier.citedreference | Prather P.L., Loh H.H., Law P.Y. ( 1994 ) Interaction of delta-opioid receptors with multiple G proteins: a non-relationship between agonist potency to inhibit adenylyl cyclase and to activate G proteins. Mol. Pharmacol. 45 997 – 1003. | en_US |
dc.identifier.citedreference | Prather P.L., McGinn T.M., Claude P.A., Liu C.L., Loh H.H., Law P.Y. ( 1995 ) Properties of a kappa-opioid receptor expressed in CHO cells: interaction with multiple G-proteins is not specific for any individual G alpha subunit and is similar to that of other opioid receptors. Brain Res. Mol. Brain Res. 29 336 – 346. | en_US |
dc.identifier.citedreference | Satoh M. & Minami M. ( 1995 ) Molecular pharmacology of the opioid receptors. Pharmacol. Ther. 68 343 – 364. | en_US |
dc.identifier.citedreference | Sanchez-Blazquez P., Rodriguez-Diaz M., DeAntonio I., Garzon J. ( 1999 ) Endomorphin-1 and endomorphin-2 show differences in their activation of Μ opioid receptor-regulated G proteins in supraspinal antinociception in mice. J. Pharmacol. Exp. Ther. 209 12 – 18. | en_US |
dc.identifier.citedreference | Seifert R., Wenzel S.K., Lee T.W., Gether U., Sanders B.E., Kobilka B.K. ( 1998 ) Different effects of Gsalpha splice variants on beta2-adrenoreceptor-mediated signaling. The beta2-adrenoreceptor coupled to the long splice variant of Gsalpha has properties of a constitutively active receptor. J. Biol. Chem. 273 5109 – 5116. | en_US |
dc.identifier.citedreference | Seifert R., Wenzel-Seifert K., Kobilka B.K. ( 1999 a ) GPCR-GΑ fusion proteins: molecular analysis of receptor-G-protein coupling. Trends Pharmacol. Sci. 20 383 – 389. | en_US |
dc.identifier.citedreference | Seifert R., Gether U., Wenzel-Seifert K., Kobilka B.K. ( 1999 b ) Effects of guanine, inosine and xanthine on Β2-adrenergic receptor/Gs interactions: evidence for multiple receptor conformations. Mol. Pharmacol. 56 348 – 358. | en_US |
dc.identifier.citedreference | Sim L.J., Liu Q., Childers S.R., Selley D.E. ( 1998 ) Endomorphin-stimulated [ 35 S]GTPΓS binding in rat brain: evidence for partial agonist activity at Μ-opioid receptors. J. Neurochem. 70 1567 – 1576. | en_US |
dc.identifier.citedreference | Stanasila L., Massotte D., Kieffer B.L., Pattus F. ( 1999 ) Expression of Δ, Μ and Κ human opioid receptors in Escherichia coli and reconstitution of the high affinity state for agonist with heterotrimeric G proteins. Eur. J. Biochem. 260 430 – 438. | en_US |
dc.identifier.citedreference | Sternini C., Spann M., Anton B., Keith D.J., Bunnett N.W., von Zastrow M., Evans C., Brecha N.C. ( 1996 ) Agonist-selective endocytosis of mu opioid receptor by neurons in vivo. Proc. Natl. Acad. Sci. USA 93 9241 – 9246. | en_US |
dc.identifier.citedreference | Taylor J.M., Jacob Mosier G., Lawton R.G., Van Dort M., Neubig R.R. ( 1996 ) Receptor and membrane interaction sites on Gbeta. A receptor-derived peptide binds to the carboxyl terminus. J. Biol. Chem. 271 3336 – 3339. | en_US |
dc.identifier.citedreference | Ueda H., Harada H., Nozaki M., Katada T., Ui M., Satoh M., Takagi H. ( 1988 ) Reconstitution of rat brain Μ opioid receptors with purified guanine nucleotide-binding regulatory proteins, Gi and Go. Proc. Natl. Acad. Sci. USA 85 7013 – 7017. | en_US |
dc.identifier.citedreference | Van den Broeck J. ( 1996 ) G-protein-coupled receptors in insect cells. Int. Rev. Cytol. 164 189 – 268. | en_US |
dc.identifier.citedreference | Waldhoer M., Wise A., Milligan G., Freissmuth M., Nanoff C. ( 1999 ) Kinetics of ternary complex formation with fusion proteins composed of the A 1 -adenosine receptor and G protein Α-subunits. J. Biol. Chem. 274 30571 – 30579. | en_US |
dc.identifier.citedreference | Whistler J.L. & von Zastrow M. ( 1998 ) Morphine-activated opioid receptors elude desensitization by beta-arrestin. Proc. Natl. Acad. Sci. USA 95 9914 – 9919. | en_US |
dc.identifier.citedreference | Wise A. & Milligan G. ( 1997 ) Rescue of functional interactions between the alpha2A-adrenoreceptor and acylation-resistant forms of Gi 1 alpha by expressing the proteins from chimeric open reading frames. J. Biol. Chem. 272 24673 – 24678. | en_US |
dc.identifier.citedreference | Yang Q. & Lanier S.M. ( 1999 ) Influence of G protein type on agonist efficacy. Mol. Pharmacol. 56 651 – 656. | en_US |
dc.identifier.citedreference | Zadina J.E., Hackler L., Ge L.J., Kastin A.J. ( 1997 ) A potent and selective endogenous agonist for the mu-opiate receptor. Nature 386 499 – 502. | en_US |
dc.identifier.citedreference | Zhang J., Ferguson S.S., Barak L.S., Bodduluri S.R., Laporte S.A., Law P.Y., Caron M.G. ( 1998 ) Role for G protein-coupled receptor kinase in agonist-specific regulation of mu-opioid receptor responsiveness. Proc. Natl. Acad. Sci. USA 95 7157 – 7166. | en_US |
dc.owningcollname | Interdisciplinary and Peer-Reviewed |
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