Agonist-Induced Endocytosis of Muscarinic Cholinergic Receptors: Relationship to Stimulated Phosphoinositide Turnover
dc.contributor.author | Sorensen, Scott D. | en_US |
dc.contributor.author | McEwen, Edward L. | en_US |
dc.contributor.author | Linseman, Daniel A. | en_US |
dc.date.accessioned | 2010-04-01T15:10:35Z | |
dc.date.available | 2010-04-01T15:10:35Z | |
dc.date.issued | 1997-04 | en_US |
dc.identifier.citation | Sorensen, Scott D.; McEwen, Edward L.; Linseman, Daniel A. (1997). "Agonist-Induced Endocytosis of Muscarinic Cholinergic Receptors: Relationship to Stimulated Phosphoinositide Turnover." Journal of Neurochemistry 68(4): 1473-1483. <http://hdl.handle.net/2027.42/65644> | en_US |
dc.identifier.issn | 0022-3042 | en_US |
dc.identifier.issn | 1471-4159 | en_US |
dc.identifier.uri | https://hdl.handle.net/2027.42/65644 | |
dc.identifier.uri | http://www.ncbi.nlm.nih.gov/sites/entrez?cmd=retrieve&db=pubmed&list_uids=9084417&dopt=citation | en_US |
dc.description.abstract | The ability of muscarinic cholinergic receptors to activate phosphoinositide turnover following agonist-induced internalization has been investigated. Incubation of SH-SY5Y neuroblastoma cells with oxotremorine-M resulted in a time-dependent endocytosis of both muscarinic receptors and Α subunits of G q and G 11 , but not of isoforms of phosphoinositide-specific phospholipase C, into a subfraction of smooth endoplasmic reticulum (V 1 ). Agonist-induced increases in diacylglycerol mass and in 32 P-phosphatidate labeling, much of which was of the tetraenoic species, were also observed in the V 1 fraction, but these increases persisted when the agonist-induced translocation of receptors into the V 1 fraction was blocked. All enzymes of the phosphoinositide cycle were detectable in the V 1 fraction. However, with the exception of phosphatidylinositol 4-kinase, none was enriched when compared with cell lysates. Both 32 P-labeling studies and enzyme assays point to a very limited capacity of this fraction to synthesize phosphatidylinositol 4,5-bisphosphate, whereas the synthesis of phosphatidylinositol 4-phosphate is robust. These results indicate that endocytosed receptors do not appear to retain their ability to activate phosphoinositide turnover. The availability of the substrate for phospholipase C, phosphatidylinositol 4,5-bisphosphate, may be one factor that limits the activity of muscarinic receptors in this subcellular compartment. | en_US |
dc.format.extent | 1383680 bytes | |
dc.format.extent | 3110 bytes | |
dc.format.mimetype | application/pdf | |
dc.format.mimetype | text/plain | |
dc.publisher | Blackwell Science Ltd | en_US |
dc.rights | Blackwell Science Inc | en_US |
dc.subject.other | G Αq/11 | en_US |
dc.subject.other | Phospholipase C isozymes | en_US |
dc.subject.other | Diacylglycerol | en_US |
dc.subject.other | Phosphatidylinositol 4-phosphate 5-kinase | en_US |
dc.subject.other | Lipid substrate availability | en_US |
dc.subject.other | Phosphatidate | en_US |
dc.title | Agonist-Induced Endocytosis of Muscarinic Cholinergic Receptors: Relationship to Stimulated Phosphoinositide Turnover | en_US |
dc.type | Article | en_US |
dc.rights.robots | IndexNoFollow | en_US |
dc.subject.hlbsecondlevel | Neurosciences | en_US |
dc.subject.hlbtoplevel | Health Sciences | en_US |
dc.description.peerreviewed | Peer Reviewed | en_US |
dc.contributor.affiliationum | Neuroscience Laboratory, Mental Health Research Institute, and Department of Pharmacology, University of Michigan, Ann Arbor, Michigan, U.S.A. | en_US |
dc.identifier.pmid | 9084417 | en_US |
dc.description.bitstreamurl | http://deepblue.lib.umich.edu/bitstream/2027.42/65644/1/j.1471-4159.1997.68041473.x.pdf | |
dc.identifier.doi | 10.1046/j.1471-4159.1997.68041473.x | en_US |
dc.identifier.source | Journal of Neurochemistry | en_US |
dc.owningcollname | Interdisciplinary and Peer-Reviewed |
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