Presence of functionally active protease-activated receptors 1 and 2 in myenteric glia
dc.contributor.author | Garrido, Rosario | en_US |
dc.contributor.author | Segura, Bradley J. | en_US |
dc.contributor.author | Zhang, Weizhen | en_US |
dc.contributor.author | Mulholland, Michael W. | en_US |
dc.date.accessioned | 2010-04-01T15:33:10Z | |
dc.date.available | 2010-04-01T15:33:10Z | |
dc.date.issued | 2002-11 | en_US |
dc.identifier.citation | Garrido, Rosario; Segura, Bradley; Zhang, Weizhen; Mulholland, Michael (2002). "Presence of functionally active protease-activated receptors 1 and 2 in myenteric glia." Journal of Neurochemistry 83(3): 556-564. <http://hdl.handle.net/2027.42/66037> | en_US |
dc.identifier.issn | 0022-3042 | en_US |
dc.identifier.issn | 1471-4159 | en_US |
dc.identifier.uri | https://hdl.handle.net/2027.42/66037 | |
dc.identifier.uri | http://www.ncbi.nlm.nih.gov/sites/entrez?cmd=retrieve&db=pubmed&list_uids=12390517&dopt=citation | en_US |
dc.description.abstract | Protease-activated receptors (PARs) belong to the family of membrane receptors coupled to G-proteins; their presence is reported in a wide variety of cells. The object of this study was to demonstrate the presence of PAR-1 and PAR-2 in myenteric glia of the guinea pig, and to elucidate the cellular mechanisms that are triggered upon receptor activation. Thrombin and PAR-1 agonist peptide (PARP-1) activate PAR-1 with a maximum mean ± SEM change in intracellular calcium concentration with respect to basal level (δ[Ca 2+ ] i ) of 183 ± 18 nm and 169 ± 6 nm, respectively. Trypsin and PAR-2 agonist peptide (PARP-2) activate PAR-2 with a maximum δ[Ca 2+ ] i of 364 ± 28 nm and 239 ± 19 nm, respectively. Inhibition of phospholipase C by U73312 (1 µm) decreased the δ[Ca 2+ ] i due to PAR-1 activation from 167 ± 10 nm to 87 ± 6 nm. The PAR-2-mediated δ[Ca 2+ ] i decreased from 193 ± 10 nm to 124 ± 8 nm when phospholipase C activity was inhibited. Blockade of sphingosine kinase with dimethylsphingosine (1 µm) decreased the δ[Ca 2+ ] i due to PAR-2 activation from 149 ± 19 nm to 67 ± 1 nm, but did not influence the PAR-1-mediated δ[Ca 2+ ] i . PAR-1 and PAR-2 were localized in myenteric glia by immunolabeling. Our results indicate that PAR-1 and PAR-2 are present in myenteric glia of the guinea pig, and their activation leads to increases in intracellular calcium via different signal transduction mechanisms that involve activation of phospholipase C and sphingosine kinase. | en_US |
dc.format.extent | 387724 bytes | |
dc.format.extent | 3110 bytes | |
dc.format.mimetype | application/pdf | |
dc.format.mimetype | text/plain | |
dc.publisher | Blackwell Science Ltd | en_US |
dc.rights | 2002 International Society for Neurochemistry | en_US |
dc.subject.other | Calcium | en_US |
dc.subject.other | Enteric Glia | en_US |
dc.subject.other | Protease-activated Receptors | en_US |
dc.subject.other | Signal Transduction | en_US |
dc.title | Presence of functionally active protease-activated receptors 1 and 2 in myenteric glia | en_US |
dc.type | Article | en_US |
dc.rights.robots | IndexNoFollow | en_US |
dc.subject.hlbsecondlevel | Neurosciences | en_US |
dc.subject.hlbtoplevel | Health Sciences | en_US |
dc.description.peerreviewed | Peer Reviewed | en_US |
dc.contributor.affiliationum | † Physiology, University of Michigan Medical Center, Ann Arbor, Michigan, USA | en_US |
dc.contributor.affiliationother | * Surgery and | en_US |
dc.identifier.pmid | 12390517 | en_US |
dc.description.bitstreamurl | http://deepblue.lib.umich.edu/bitstream/2027.42/66037/1/j.1471-4159.2002.01119.x.pdf | |
dc.identifier.doi | 10.1046/j.1471-4159.2002.01119.x | en_US |
dc.identifier.source | Journal of Neurochemistry | en_US |
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dc.owningcollname | Interdisciplinary and Peer-Reviewed |
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