Synaptic Vesicular Glutamate Uptake: Modulation by a Synaptosomal Cytosolic Factor
dc.contributor.author | Lobur, Anne T. | en_US |
dc.contributor.author | Kish, Phillip E. | en_US |
dc.contributor.author | Ueda, Tetsufumi | en_US |
dc.date.accessioned | 2010-04-01T15:42:18Z | |
dc.date.available | 2010-04-01T15:42:18Z | |
dc.date.issued | 1990-05 | en_US |
dc.identifier.citation | Lobur, Anne T.; Kish, Phillip E.; Ueda, Tetsufumi (1990). "Synaptic Vesicular Glutamate Uptake: Modulation by a Synaptosomal Cytosolic Factor." Journal of Neurochemistry 54(5): 1614-1618. <http://hdl.handle.net/2027.42/66195> | en_US |
dc.identifier.issn | 0022-3042 | en_US |
dc.identifier.issn | 1471-4159 | en_US |
dc.identifier.uri | https://hdl.handle.net/2027.42/66195 | |
dc.identifier.uri | http://www.ncbi.nlm.nih.gov/sites/entrez?cmd=retrieve&db=pubmed&list_uids=1969936&dopt=citation | en_US |
dc.description.abstract | We have demonstrated previously that L-glutamate is taken up into isolated synaptic vesicles in an ATP-dependent manner, supporting the neurotransmitter role of this acidic amino acid. We now report that a nerve terminal cytosolic factor inhibits the ATP-dependent vesicular uptake of glutamate in a dose-dependent manner. This factor appears to be a protein with a molecular weight >100,000, as estimated by size exclusion chromatography, and is precipitated by ammonium sulfate (40% saturation). The inhibitory factor is inactivated by heating to 100°C. Proteolytic digestion of the ammonium sulfate fraction by trypsin or chymotrypsin did not reduce, but rather increased slightly, the inhibition of glutamate uptake. Unlike the native factor, the digest retained inhibitory activity after heating, suggesting that proteolytic digestion may generate active fragments. The inhibition of ATP-dependent vesicular glutamate uptake is not species-specific, as the factor obtained from both rat and bovine brains produced an equal degree of inhibition of glutamate uptake into vesicles of each species. These observations raise the possibility that vesicular uptake of glutamate may be regulated by an endogenous factor in vivo. | en_US |
dc.format.extent | 577011 bytes | |
dc.format.extent | 3110 bytes | |
dc.format.mimetype | application/pdf | |
dc.format.mimetype | text/plain | |
dc.publisher | Blackwell Publishing Ltd | en_US |
dc.rights | 1990 International Society for Neurochemistry Ltd. | en_US |
dc.subject.other | Glutamate Uptake | en_US |
dc.subject.other | Vesicles | en_US |
dc.subject.other | Cytosolic Factor | en_US |
dc.title | Synaptic Vesicular Glutamate Uptake: Modulation by a Synaptosomal Cytosolic Factor | en_US |
dc.type | Article | en_US |
dc.rights.robots | IndexNoFollow | en_US |
dc.subject.hlbsecondlevel | Neurosciences | en_US |
dc.subject.hlbtoplevel | Health Sciences | en_US |
dc.description.peerreviewed | Peer Reviewed | en_US |
dc.contributor.affiliationum | * Department of Pharmacology, The University of Michigan, Ann Arbor, Michigan, U.S.A. | en_US |
dc.contributor.affiliationum | † Department of Psychiatry, The University of Michigan, Ann Arbor, Michigan, U.S.A. | en_US |
dc.contributor.affiliationum | † Mental Health Research Institute, The University of Michigan, Ann Arbor, Michigan, U.S.A. | en_US |
dc.identifier.pmid | 1969936 | en_US |
dc.description.bitstreamurl | http://deepblue.lib.umich.edu/bitstream/2027.42/66195/1/j.1471-4159.1990.tb01212.x.pdf | |
dc.identifier.doi | 10.1111/j.1471-4159.1990.tb01212.x | en_US |
dc.identifier.source | Journal of Neurochemistry | en_US |
dc.identifier.citedreference | Ascher, P. and Nowak, L. ( 1987 ) Electrophysiological studies of NMDA receptors. Trends Neurosci. 10, 284 – 288. | en_US |
dc.identifier.citedreference | Cotman, C. W. and Iversen, L. L. ( 1987 ) Excitatory amino acids in the brain–focus on the NMDA receptors. Trends Neurosci. 10, 263 – 264. | en_US |
dc.identifier.citedreference | Cotman, C. W., Monahan, D. T., Ottersen, O. P., and Storm-Mathisen, J. ( 1987 ) Anatomical organization of excitatory amino acid receptors and their pathways. Trends Neurosci. 10, 273 – 279. | en_US |
dc.identifier.citedreference | Cotman, C. W., Bridges, R. J., Taube, J. S., Clark, A. S., Geddes, J. W., and Monaghan, D. T. ( 1989 ) The role of the NMDA receptor in central nervous system plasticity and pathology. J. NIH Res. 1, 65 – 74. | en_US |
dc.identifier.citedreference | Fagg, G. E., Foster, A. C., and Ganong, A. H. ( 1986 ) Excitatory amino acid synaptic mechanisms and neurological function. Trends Pharmacol. Sci. 7, 357 – 363. | en_US |
dc.identifier.citedreference | Fischer-Bovenkerk, C., Kish, P. E., and Ueda, T. ( 1988 ) ATP-dependent glutamate uptake into synaptic vesicles from cerebellar mutant mice. J. Neurochem. 51, 1054 – 1059. | en_US |
dc.identifier.citedreference | Fonnum, F. ( 1984 ) Glutamate: a neurotransmitter in mammalian brain. J. Neurochem. 42, 1 – 11. | en_US |
dc.identifier.citedreference | Greenamyre, J. T. ( 1986 ) The role of glutamate in neurotransmission and in neurologic disease. Arch. Neurol. 43, 1058 – 1063. | en_US |
dc.identifier.citedreference | Greenamyre, J. T., Young, A. B., and Penney, J. B. ( 1984 ) Quantitative autoradiographic distribution of L-[H 3 ]glutamate-binding sites in rat central nervous system. J. Neurosci. 4, 2133 – 2144. | en_US |
dc.identifier.citedreference | Kemp, J. A., Foster, A. C., and Wong, E. H. F. ( 1987 ) Non-competitive antagonists of excitatory amino acid receptors. Trends Neurosci. 10, 294 – 298. | en_US |
dc.identifier.citedreference | Kish, P. E. and Ueda, T. ( 1989 ) Glutamate accumulation into synaptic vesicles, in Methods in Enzymology, Biomembranes, Part U: Biological Transport Vol. 174, Cellular and Subcellular Transport: Eukaryotic (Non-Epithelial) Cells ( Fleischer, S. and Fleischer, B., eds ), pp. 9 – 25. | en_US |
dc.identifier.citedreference | Kish, P. E., Kim, S. Y., and Ueda, T. ( 1989 ) Ontogeny of glutamate accumulating activity in rat brain synaptic vesicles. Neurosci. Lett. 97, 185 – 190. | en_US |
dc.identifier.citedreference | Lobur, A. T., Kish, P. E., and Ueda, T. ( 1988 ) Evidence for a synaptosomal cytosolic factor modulating vesicular glutamate uptake. Soc. Neurosci. Abstr. 14, 275.18. | en_US |
dc.identifier.citedreference | Lowry, O. H., Rosebrough, N. J., Farr, A. L., and Randall, R. J. ( 1951 ) Protein measurement with Folin phenol reagent. J. Biol. Chem. 193, 265 – 275. | en_US |
dc.identifier.citedreference | Maycox, P. R., Deckwerth, T., Hell, J. W., and Jahn, R. ( 1988 ) Glutamate uptake by brain synaptic vesicles. J. Biol. Chem. 263, 15423 – 15428. | en_US |
dc.identifier.citedreference | Meldrum, B. ( 1985 ) Possible therapeutic applications of antagonists of excitatory amino acid neurotransmitters. Clin. Sci. 68, 113 – 122. | en_US |
dc.identifier.citedreference | Naito, S. and Ueda, T. ( 1983 ) Adenosine triphosphate-dependent up-take of glutamate into Protein I-associated synaptic vesicles. J. Biol. Chem. 258, 696 – 699. | en_US |
dc.identifier.citedreference | Naito, S. and Ueda, T. ( 1985 ) Characterization of glutamate uptake into synaptic vesicles. J. Neurochem. 44, 99 – 109. | en_US |
dc.identifier.citedreference | Nicholls, D. G. and Sihra, T. ( 1986 ) Synaptosomes possess an exocytotic pool of glutamate. Nature 321, 772 – 773. | en_US |
dc.identifier.citedreference | Olney, J. W., Collins, R. C., and Sloviter, R. S. ( 1986 ) Excitotoxic mechanism of epileptic brain damage. Adv. Neurol. 44, 857 – 877. | en_US |
dc.identifier.citedreference | Parsons, S. M., Bahr, B. A., Gracz, L. M., Kaufman, R., Kornreich, W. D., Nilsson, L., and Rogers, G. A. ( 1987 ) Acetylcholine transport: fundamental properties and effects of pharmacologic agents. Ann. NY Acad. Sci. 493, 220 – 233. | en_US |
dc.identifier.citedreference | Rand, M. J. and Jurevics, H. ( 1977 ) The pharmacology of raulwolfia alkaloids. Handb. Exp. Pharmacol. 39, 77 – 99. | en_US |
dc.identifier.citedreference | Robinson, M. B. and Coyle, J. T. ( 1987 ) Glutamate and related acidic excitatory neurotransmitters: from basic science to clinical application. FASEB J. 1, 446 – 455. | en_US |
dc.identifier.citedreference | Rothman, S. M. and Olney, J. W. ( 1987 ) Excitotoxicity and the NMDA receptor. Trends Neurosci. 10, 299 – 302. | en_US |
dc.identifier.citedreference | Shank, R. P. and Campbell, G. Le M. ( 1984 ) Glutamine, glutamate, and other possible regulators of Α -ketoglutarate and malate up-take by synaptic terminals. J. Neurochem. 42, 1162 – 1169. | en_US |
dc.identifier.citedreference | Storm-Mathisen, J., Leknes, A. K., Bore, A. T., Vaaland, J. L., Edminson, P., Haug, F.-M. S., and Ottersen, O. P. ( 1983 ) First visualization of glutamate and GABA in neurons by immunocytochemistry. Nature 301, 517 – 520. | en_US |
dc.identifier.citedreference | Ueda, T. ( 1986 ) Glutamate transport in the synaptic vesicle, in Excitatory Amino Acids ( Roberts, P. J., Storm-Mathisen, J., and Bradford, H. F., eds ), pp. 173 – 195. Macmillan Press, London. | en_US |
dc.identifier.citedreference | Watkins, J. C. and Evans, R. H. ( 1981 ) Excitatory amino acid transmitters. Annu. Rev. Pharmacol. Toxicol. 21, 165 – 204. | en_US |
dc.identifier.citedreference | Watkins, J. C. and Olverman, H. J. ( 1987 ) Agonists and antagonists for excitatory amino acid receptors. Trends Neurosci. 10, 265 – 272. | en_US |
dc.identifier.citedreference | Weiner, N. ( 1970 ) Regulation of norepinephrine biosynthesis. Annu. Rev. Pharmacol. 75 ( Suppl. ). 87 – 94. | en_US |
dc.owningcollname | Interdisciplinary and Peer-Reviewed |
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