Glutamate Uptake into Synaptic Vesicles: Competitive Inhibition by Bromocriptine
dc.contributor.author | Carlson, Martha D. | en_US |
dc.contributor.author | Kish, Phillip E. | en_US |
dc.contributor.author | Ueda, Tetsufumi | en_US |
dc.date.accessioned | 2010-04-01T15:46:55Z | |
dc.date.available | 2010-04-01T15:46:55Z | |
dc.date.issued | 1989-12 | en_US |
dc.identifier.citation | Carlson, Martha D.; Kish, Phillip E.; Ueda, Tetsufumi (1989). "Glutamate Uptake into Synaptic Vesicles: Competitive Inhibition by Bromocriptine." Journal of Neurochemistry 53(6): 1889-1894. <http://hdl.handle.net/2027.42/66274> | en_US |
dc.identifier.issn | 0022-3042 | en_US |
dc.identifier.issn | 1471-4159 | en_US |
dc.identifier.uri | https://hdl.handle.net/2027.42/66274 | |
dc.identifier.uri | http://www.ncbi.nlm.nih.gov/sites/entrez?cmd=retrieve&db=pubmed&list_uids=2809599&dopt=citation | en_US |
dc.description.abstract | The ATP-dependent uptake of l-glutamate into synaptic vesicles has been well characterized, implicating a key role for synaptic vesicles in glutamatergic neurotransmission. In the present study, we provide evidence that vesicular glutamate uptake is selectively inhibited by the pep-tide-containing halogenated ergot bromocriptine. It is the most potent inhibitor of the agents tested; the IC 5 o was de-termined to be 22 Μ M . The uptake was also inhibited by other ergopeptines such as ergotamine and ergocristine, but with less potency. Ergots devoid of the peptide moiety, however, such as ergonovine, lergotrile, and methysergide, had little or no effect. Although bromocriptine is known to elicit dopaminergic and serotonergic effects, its inhibitory effect on vesicular glutamate uptake was not mimicked by agents known to interact with dopamine and serotonin receptors. Kinetic data suggest that bromocriptine competes with glutamate for the glutamate binding site on the glutamate trans-locator. It is proposed that this inhibitor could be useful as a prototype probe in identifying and characterizing the vesicular glutamate translocator, as well as in developing a more specific inhibitor of the transport system. | en_US |
dc.format.extent | 627324 bytes | |
dc.format.extent | 3110 bytes | |
dc.format.mimetype | application/pdf | |
dc.format.mimetype | text/plain | |
dc.publisher | Blackwell Publishing Ltd | en_US |
dc.rights | 1989 International Society for Neurochemistry | en_US |
dc.subject.other | Glutamate | en_US |
dc.subject.other | Synaptic Vesicle | en_US |
dc.subject.other | ATP-dependent Uptake | en_US |
dc.subject.other | Ergots | en_US |
dc.subject.other | Competitive Inhibition | en_US |
dc.subject.other | Glutamate Translocator | en_US |
dc.title | Glutamate Uptake into Synaptic Vesicles: Competitive Inhibition by Bromocriptine | en_US |
dc.type | Article | en_US |
dc.rights.robots | IndexNoFollow | en_US |
dc.subject.hlbsecondlevel | Neurosciences | en_US |
dc.subject.hlbtoplevel | Health Sciences | en_US |
dc.description.peerreviewed | Peer Reviewed | en_US |
dc.contributor.affiliationum | * The Department of Pharmacology, The University of Michigan, Ann Arbor, Michigan, U.S.A. | en_US |
dc.contributor.affiliationum | The Department of Psychiatry, The University of Michigan, Ann Arbor, Michigan, U.S.A. | en_US |
dc.contributor.affiliationum | Mental Health Research Institute, The University of Michigan, Ann Arbor, Michigan, U.S.A. | en_US |
dc.identifier.pmid | 2809599 | en_US |
dc.description.bitstreamurl | http://deepblue.lib.umich.edu/bitstream/2027.42/66274/1/j.1471-4159.1989.tb09258.x.pdf | |
dc.identifier.doi | 10.1111/j.1471-4159.1989.tb09258.x | en_US |
dc.identifier.source | Journal of Neurochemistry | en_US |
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dc.owningcollname | Interdisciplinary and Peer-Reviewed |
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