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Synapsin I Is Associated with Cholinergic Nerve Terminals in the Electric Organs of Torpedo, Electrophorus , and Malapterurus and Copurifies with Torpedo Synaptic Vesicles
dc.contributor.authorVolknandt, W.en_US
dc.contributor.authorNaito, Shigetakaen_US
dc.contributor.authorUeda, Tetsufumien_US
dc.contributor.authorZimmermann, H.en_US
dc.date.accessioned2010-04-01T15:50:50Z
dc.date.available2010-04-01T15:50:50Z
dc.date.issued1987-08en_US
dc.identifier.citationVolknandt, W.; Naito, S.; Ueda, T.; Zimmermann, H. (1987). "Synapsin I Is Associated with Cholinergic Nerve Terminals in the Electric Organs of Torpedo, Electrophorus , and Malapterurus and Copurifies with Torpedo Synaptic Vesicles." Journal of Neurochemistry 49(2): 342-347. <http://hdl.handle.net/2027.42/66342>en_US
dc.identifier.issn0022-3042en_US
dc.identifier.issn1471-4159en_US
dc.identifier.urihttps://hdl.handle.net/2027.42/66342
dc.identifier.urihttp://www.ncbi.nlm.nih.gov/sites/entrez?cmd=retrieve&db=pubmed&list_uids=3110371&dopt=citationen_US
dc.description.abstractUsing an affinity-purified monospecific polyclonal antibody against bovine brain synapsin I, the distribution of antigenically related proteins was investigated in the electric organs of the three strongly electric fish Torpedo marmorata, Electrophorus electricus, Malapterurus electricus and in the rat diaphragm. On application of indirect fluorescein isothiocyanate-immunofluorescence and using Α-bungarotoxin for identification of synaptic sites, intense and very selective staining of nerve terminals was found in all of these tissues. Immunotransfer blots of tissue homogenates revealed specific bands whose molecular weights are similar to those of synapsin Ia and synapsin Ib. Moreover, synapsin I-like proteins are still attached to the synaptic vesicles that were isolated in isotonic glycine solution from Torpedo electric organ by density gradient centrifugation and chromatography on Sephacryl-1000. Our results suggest that synapsin I-like proteins are also associated with cholinergic synaptic vesicles of electric organs and that the electric organ may be an ideal source for studying further the functional and molecular properties of synapsin I.en_US
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dc.format.extent3110 bytes
dc.format.mimetypeapplication/pdf
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dc.publisherBlackwell Publishing Ltden_US
dc.rights1987 International Society for Neurochemistry Ltd.en_US
dc.subject.otherSynapsin Ien_US
dc.subject.otherProtein Ien_US
dc.subject.otherTorpedoen_US
dc.subject.otherSynaptic Vesiclesen_US
dc.subject.otherAcetylcholineen_US
dc.subject.otherElectrophorusen_US
dc.subject.otherMalapterurusen_US
dc.titleSynapsin I Is Associated with Cholinergic Nerve Terminals in the Electric Organs of Torpedo, Electrophorus , and Malapterurus and Copurifies with Torpedo Synaptic Vesiclesen_US
dc.typeArticleen_US
dc.rights.robotsIndexNoFollowen_US
dc.subject.hlbsecondlevelNeurosciencesen_US
dc.subject.hlbtoplevelHealth Sciencesen_US
dc.description.peerreviewedPeer Revieweden_US
dc.contributor.affiliationum* Mental Health Research Institute, University of Michigan, Ann Arbor, Michigan, U.S.A.en_US
dc.contributor.affiliationotherAK Neurochemie, Zoologisches Institut der J. W. Goethe-UniversitÄt, Frankfurt am Main, F.R.G.en_US
dc.identifier.pmid3110371en_US
dc.description.bitstreamurlhttp://deepblue.lib.umich.edu/bitstream/2027.42/66342/1/j.1471-4159.1987.tb02871.x.pdf
dc.identifier.doi10.1111/j.1471-4159.1987.tb02871.xen_US
dc.identifier.sourceJournal of Neurochemistryen_US
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dc.identifier.citedreferenceVolknandt W. and Zimmermann H. ( 1986 ) Acetylcholine, ATP and proteoglycan are common to synaptic vesicles isolated from the electric organs of electric eel and electric catfish as well as from rat diaphragm. J. Neurochem. 47, 1449 – 1462.en_US
dc.identifier.citedreferenceZimmermann H. ( 1982 ) Biochemistry of the isolated cholinergic vesicles, in Neurotransmitter Vesicles ( Klein R. L., Lagercrantz H., and Zimmermann H., eds ), pp. 271 – 304. Academic Press, London.en_US
dc.owningcollnameInterdisciplinary and Peer-Reviewed


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