N-System Amino Acid Transport at the Blood-CSF Barrier

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dc.contributor.author Xiang, Jianming en_US
dc.date.accessioned 2010-04-01T15:55:23Z
dc.date.available 2010-04-01T15:55:23Z
dc.date.issued 1995-12 en_US
dc.identifier.citation Xiang, Jianming (1995). "N-System Amino Acid Transport at the Blood-CSF Barrier." Journal of Neurochemistry 65(6): 2571-2576. <http://hdl.handle.net/2027.42/66421> en_US
dc.identifier.issn 0022-3042 en_US
dc.identifier.issn 1471-4159 en_US
dc.identifier.uri http://hdl.handle.net/2027.42/66421
dc.identifier.uri http://www.ncbi.nlm.nih.gov/sites/entrez?cmd=retrieve&db=pubmed&list_uids=7595553&dopt=citation en_US
dc.description.abstract Despite l-glutamine being the most abundant amino acid in CSF, the mechanisms of its transport at the choroid plexus have not been fully elucidated. This study examines the role of L-, A-, ASC-, and N-system amino acid transporters in l-[ 14 C]glutamine uptake into isolated rat choroid plexus. In the absence of competing amino acids, approximately half the glutamine uptake was via a Na + -dependent mechanism. The Na + -independent uptake was inhibited by 2-amino-2-norbornane carboxylic acid, indicating that it is probably via an L-system transporter. Na + -dependent uptake was inhibited neither by the A-system substrate Α-(methylamino)isobutyric acid nor by the ASC-system substrate cysteine. It was inhibited by histidine, asparagine, and l-glutamate Γ-hydroxamate, three N-system substrates. Replacement of Na + with Li + had little effect on uptake, another feature of N-system amino acid transport. These data therefore indicate that N-system amino acid transport is present at the choroid plexus. The V max and K max for glutamine transport by this system were 8.1 ± 0.3 nmol/mg/min and 3.3 ± 0.4 m M , respectively. This system may play an important role in the control of CSF glutamine, particularly when the CSF glutamine level is elevated as in hepatic encephalopathy. en_US
dc.format.extent 559193 bytes
dc.format.extent 3110 bytes
dc.format.mimetype application/pdf
dc.format.mimetype text/plain
dc.publisher Blackwell Science Ltd en_US
dc.rights Blackwell Science Inc en_US
dc.subject.other Choroid Plexus en_US
dc.subject.other Glutamine en_US
dc.subject.other Histidine en_US
dc.subject.other Rat en_US
dc.subject.other Influx en_US
dc.title N-System Amino Acid Transport at the Blood-CSF Barrier en_US
dc.type Article en_US
dc.rights.robots IndexNoFollow en_US
dc.subject.hlbsecondlevel Neurosciences en_US
dc.subject.hlbtoplevel Health Sciences en_US
dc.description.peerreviewed Peer Reviewed en_US
dc.contributor.affiliationum Department of Surgery, University of Michigan, Ann Arbor, Michigan, U.S.A. en_US
dc.identifier.pmid 7595553 en_US
dc.description.bitstreamurl http://deepblue.lib.umich.edu/bitstream/2027.42/66421/1/j.1471-4159.1995.65062571.x.pdf
dc.identifier.doi 10.1046/j.1471-4159.1995.65062571.x en_US
dc.identifier.source Journal of Neurochemistry en_US
dc.owningcollname Interdisciplinary and Peer-Reviewed
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