N-System Amino Acid Transport at the Blood-CSF Barrier
dc.contributor.author | Xiang, Jianming | en_US |
dc.date.accessioned | 2010-04-01T15:55:23Z | |
dc.date.available | 2010-04-01T15:55:23Z | |
dc.date.issued | 1995-12 | en_US |
dc.identifier.citation | Xiang, Jianming (1995). "N-System Amino Acid Transport at the Blood-CSF Barrier." Journal of Neurochemistry 65(6): 2571-2576. <http://hdl.handle.net/2027.42/66421> | en_US |
dc.identifier.issn | 0022-3042 | en_US |
dc.identifier.issn | 1471-4159 | en_US |
dc.identifier.uri | https://hdl.handle.net/2027.42/66421 | |
dc.identifier.uri | http://www.ncbi.nlm.nih.gov/sites/entrez?cmd=retrieve&db=pubmed&list_uids=7595553&dopt=citation | en_US |
dc.description.abstract | Despite l-glutamine being the most abundant amino acid in CSF, the mechanisms of its transport at the choroid plexus have not been fully elucidated. This study examines the role of L-, A-, ASC-, and N-system amino acid transporters in l-[ 14 C]glutamine uptake into isolated rat choroid plexus. In the absence of competing amino acids, approximately half the glutamine uptake was via a Na + -dependent mechanism. The Na + -independent uptake was inhibited by 2-amino-2-norbornane carboxylic acid, indicating that it is probably via an L-system transporter. Na + -dependent uptake was inhibited neither by the A-system substrate Α-(methylamino)isobutyric acid nor by the ASC-system substrate cysteine. It was inhibited by histidine, asparagine, and l-glutamate Γ-hydroxamate, three N-system substrates. Replacement of Na + with Li + had little effect on uptake, another feature of N-system amino acid transport. These data therefore indicate that N-system amino acid transport is present at the choroid plexus. The V max and K max for glutamine transport by this system were 8.1 ± 0.3 nmol/mg/min and 3.3 ± 0.4 m M , respectively. This system may play an important role in the control of CSF glutamine, particularly when the CSF glutamine level is elevated as in hepatic encephalopathy. | en_US |
dc.format.extent | 559193 bytes | |
dc.format.extent | 3110 bytes | |
dc.format.mimetype | application/pdf | |
dc.format.mimetype | text/plain | |
dc.publisher | Blackwell Science Ltd | en_US |
dc.rights | Blackwell Science Inc | en_US |
dc.subject.other | Choroid Plexus | en_US |
dc.subject.other | Glutamine | en_US |
dc.subject.other | Histidine | en_US |
dc.subject.other | Rat | en_US |
dc.subject.other | Influx | en_US |
dc.title | N-System Amino Acid Transport at the Blood-CSF Barrier | en_US |
dc.type | Article | en_US |
dc.rights.robots | IndexNoFollow | en_US |
dc.subject.hlbsecondlevel | Neurosciences | en_US |
dc.subject.hlbtoplevel | Health Sciences | en_US |
dc.description.peerreviewed | Peer Reviewed | en_US |
dc.contributor.affiliationum | Department of Surgery, University of Michigan, Ann Arbor, Michigan, U.S.A. | en_US |
dc.identifier.pmid | 7595553 | en_US |
dc.description.bitstreamurl | http://deepblue.lib.umich.edu/bitstream/2027.42/66421/1/j.1471-4159.1995.65062571.x.pdf | |
dc.identifier.doi | 10.1046/j.1471-4159.1995.65062571.x | en_US |
dc.identifier.source | Journal of Neurochemistry | en_US |
dc.owningcollname | Interdisciplinary and Peer-Reviewed |
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