Receptor-Coupled Phosphoinositide Hydrolysis in Human Retinal Pigment Epithelium
dc.contributor.author | Feldman, Eva L. | en_US |
dc.contributor.author | Randolph, Ann E. | en_US |
dc.contributor.author | Johnston, Gregory C. | en_US |
dc.contributor.author | DelMonte, Monte A. | en_US |
dc.contributor.author | Greene, Douglas A. | en_US |
dc.date.accessioned | 2010-04-01T15:55:55Z | |
dc.date.available | 2010-04-01T15:55:55Z | |
dc.date.issued | 1991-06 | en_US |
dc.identifier.citation | Feldman, Eva L.; Randolph, Ann E.; Johnston, Gregory C.; DelMonte, Monte A.; Greene, Douglas A. (1991). "Receptor-Coupled Phosphoinositide Hydrolysis in Human Retinal Pigment Epithelium." Journal of Neurochemistry 56(6): 2094-2100. <http://hdl.handle.net/2027.42/66430> | en_US |
dc.identifier.issn | 0022-3042 | en_US |
dc.identifier.issn | 1471-4159 | en_US |
dc.identifier.uri | https://hdl.handle.net/2027.42/66430 | |
dc.identifier.uri | http://www.ncbi.nlm.nih.gov/sites/entrez?cmd=retrieve&db=pubmed&list_uids=1851211&dopt=citation | en_US |
dc.description.abstract | Carbachol and histamine stimulated phosphoinositide (PPI) hydrolysis in cultured human retinal pigment epithelium (RPE), as reflected by an accumulation of 3 H-inositol phosphates in the presence of 10 m M Li + . Carbachol increased PPI hydrolysis to greater than 600% of basal with an EC 50 of 60 Μ M ; stimulation was linear up to 60 min. This activation likely occurred via the M 3 muscarinic cholinergic receptor based on the IC 50 values for 4-diphenylacetoxy- N -methylpiperidine methiodide (0.47 n M ), pirenzepine (280 n M ), and 11-[[2-[(diethylamino)methyl]-1-piperidinyl]-acetyl]-5,11-dihydro-6 H -pyrido[2,3- b ][1,4]benzodiazepin-6-one (1.4 Μ M ). Carbachol-mediated PPI hydrolysis was decreased by 80% in the absence of extracellular Ca 2+ . Histamine stimulated PPI turnover in a linear manner by 180% with an EC 50 of 20 Μ M by the H 1 histaminergic receptor. Serotonin, glutamate, norepinephrine, and dopamine were inactive. In human RPE, the resting cytoplasmic Ca 2+ concentration, as determined by fura-2 fluorescence, was 138 ± 24 n M . On the addition of carbachol, there was a 180% increase in peak intracellular Ca 2+ ; addition of histamine increased intracellular Ca 2+ by 187%. These results suggest receptor-mediated, inositol lipid hydrolysis is coupled to intracellular Ca 2+ flux in human RPE. | en_US |
dc.format.extent | 763050 bytes | |
dc.format.extent | 3110 bytes | |
dc.format.mimetype | application/pdf | |
dc.format.mimetype | text/plain | |
dc.publisher | Blackwell Publishing Ltd | en_US |
dc.rights | 1991 International Society for Neurochemistry | en_US |
dc.subject.other | Human Pigment Retinal Epithelium | en_US |
dc.subject.other | M 3 Muscarinic Receptor | en_US |
dc.subject.other | H 1 Histaminergic Receptor | en_US |
dc.subject.other | Phosphoinositide Hydrolysis | en_US |
dc.subject.other | Ca 2+ Activation | en_US |
dc.subject.other | Fura-2 AM | en_US |
dc.title | Receptor-Coupled Phosphoinositide Hydrolysis in Human Retinal Pigment Epithelium | en_US |
dc.type | Article | en_US |
dc.rights.robots | IndexNoFollow | en_US |
dc.subject.hlbsecondlevel | Neurosciences | en_US |
dc.subject.hlbtoplevel | Health Sciences | en_US |
dc.description.peerreviewed | Peer Reviewed | en_US |
dc.contributor.affiliationum | Department of Neurology, University of Michigan, Ann Arbor, Michigan, U.S.A. | en_US |
dc.contributor.affiliationum | * Department of Ophthalmology, University of Michigan, Ann Arbor, Michigan, U.S.A. | en_US |
dc.contributor.affiliationum | † Internal Medicine, University of Michigan, Ann Arbor, Michigan, U.S.A. | en_US |
dc.identifier.pmid | 1851211 | en_US |
dc.description.bitstreamurl | http://deepblue.lib.umich.edu/bitstream/2027.42/66430/1/j.1471-4159.1991.tb03471.x.pdf | |
dc.identifier.doi | 10.1111/j.1471-4159.1991.tb03471.x | en_US |
dc.identifier.source | Journal of Neurochemistry | en_US |
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dc.owningcollname | Interdisciplinary and Peer-Reviewed |
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