ATP-Dependent Glutamate Uptake into Synaptic Vesicles from Cerebellar Mutant Mice
dc.contributor.author | Fischer-Bovenkerk, Carolyn | en_US |
dc.contributor.author | Kish, Phillip E. | en_US |
dc.contributor.author | Ueda, Tetsufumi | en_US |
dc.date.accessioned | 2010-04-01T15:57:01Z | |
dc.date.available | 2010-04-01T15:57:01Z | |
dc.date.issued | 1988-10 | en_US |
dc.identifier.citation | Fischer-Bovenkerk, Carolyn; Kish, Phillip E.; Ueda, Tetsufumi (1988). "ATP-Dependent Glutamate Uptake into Synaptic Vesicles from Cerebellar Mutant Mice." Journal of Neurochemistry 51(4): 1054-1059. <http://hdl.handle.net/2027.42/66449> | en_US |
dc.identifier.issn | 0022-3042 | en_US |
dc.identifier.issn | 1471-4159 | en_US |
dc.identifier.uri | https://hdl.handle.net/2027.42/66449 | |
dc.identifier.uri | http://www.ncbi.nlm.nih.gov/sites/entrez?cmd=retrieve&db=pubmed&list_uids=2901460&dopt=citation | en_US |
dc.description.abstract | The ATP-dependent glutamate uptake system in synaptic vesicles prepared from mouse cerebellum was characterized, and the levels of glutamate uptake were investigated in the cerebellar mutant mice, staggerer and weaver, whose main defect is the loss of cerebellar granule cells, and the nervous mutant, whose main defect is the loss of Purkinje cells. The ATP-dependent glutamate uptake is stimulated by low concentrations of chloride, is insensitive to aspartate, and is inhibited by agents known to dissipate the electrochemical proton gradient. These properties are similar to those of the glutamate uptake system observed in the highly purified synaptic vesicles prepared from bovine cortex. The ATP-dependent glutamate uptake system is reduced by 68% in the staggerer and 57–67% in the weaver mutant; these reductions parallel the substantial loss of granule cells in those mutants. In contrast, the cerebellar levels of glutamate uptake are not altered significantly in the nervous mutant, which has lost Purkinje cells, but not granule cells. In view of evidence that granule cells are glutamatergic neurons and Purkinje cells are GABAergic neurons, these observations support the notion that the ATP-dependent glutamate uptake system is present in synaptic vesicles of glutamatergic neurons. | en_US |
dc.format.extent | 587376 bytes | |
dc.format.extent | 3110 bytes | |
dc.format.mimetype | application/pdf | |
dc.format.mimetype | text/plain | |
dc.publisher | Blackwell Publishing Ltd | en_US |
dc.rights | 1988 International Society for Neurochemistry Ltd. | en_US |
dc.subject.other | Glutamate | en_US |
dc.subject.other | Synaptic Vesicle | en_US |
dc.subject.other | Staggerer | en_US |
dc.subject.other | Weaver | en_US |
dc.subject.other | Nervous | en_US |
dc.subject.other | Granule Cell | en_US |
dc.title | ATP-Dependent Glutamate Uptake into Synaptic Vesicles from Cerebellar Mutant Mice | en_US |
dc.type | Article | en_US |
dc.rights.robots | IndexNoFollow | en_US |
dc.subject.hlbsecondlevel | Neurosciences | en_US |
dc.subject.hlbtoplevel | Health Sciences | en_US |
dc.description.peerreviewed | Peer Reviewed | en_US |
dc.contributor.affiliationum | Departments of Pharmacology and Psychiatry, The University of Michigan, Ann Arbor, Michigan, U.S.A. | en_US |
dc.contributor.affiliationother | Mental Health Research Institute . | en_US |
dc.identifier.pmid | 2901460 | en_US |
dc.description.bitstreamurl | http://deepblue.lib.umich.edu/bitstream/2027.42/66449/1/j.1471-4159.1988.tb03068.x.pdf | |
dc.identifier.doi | 10.1111/j.1471-4159.1988.tb03068.x | en_US |
dc.identifier.source | Journal of Neurochemistry | en_US |
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dc.owningcollname | Interdisciplinary and Peer-Reviewed |
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