Cytotoxic Effects of Resin Components on Cultured Mammalian Fibroblasts
dc.contributor.author | Hanks, Carl T. | en_US |
dc.contributor.author | Strawn, S. E. (Susan E.) | en_US |
dc.contributor.author | Watahai, J. C. | en_US |
dc.contributor.author | Craig, Robert G. | en_US |
dc.date.accessioned | 2010-04-13T18:41:30Z | |
dc.date.available | 2010-04-13T18:41:30Z | |
dc.date.issued | 1991 | en_US |
dc.identifier.citation | Hanks, C.T.; Strawn, S.E.; Watahai, J.C.; Craig, R.G. (1991). "Cytotoxic Effects of Resin Components on Cultured Mammalian Fibroblasts." Journal of Dental Research 11(70): 1450-1455. <http://hdl.handle.net/2027.42/66623> | en_US |
dc.identifier.issn | 0022-0345 | en_US |
dc.identifier.uri | https://hdl.handle.net/2027.42/66623 | |
dc.description.abstract | The objectives of this study were to determine the cytotoxic concentrations of 11 components of resin composites on monolayers of cultured Balb/c 3T3 fibroblasts, to study the inhibitory effects of these components on DNA synthesis, total protein content, and protein synthesis, and to determine whether effects were reversible when the components were withdrawn from the medium. These data were reported as concentrations which inhibited 10% (ID10) and 50% (ID50) of a particular metabolic process as well as the range of concentrations over which cell metabolism was irreversibly inhibited. For any individual component, the ID50 values for all three metabolic parameters were of the same magnitude. The same was true for the ranges of irreversibility. Ethoxylated Bis-phenol A dimethacrylate (E-BPA) was the most toxic molecule of the group (ID50 being between 1 and 10 μmol/L). The ID50 concentrations for three of the components, including Bis-GMA, UDMA, TEGDMA, and Bis-phenol A, ranged between 10 and 100 μmol/L, while the ID 50 values of three components (N,N dihydroxyethyl-p-toluidine, camphoroquinone, and N,N dimethylaminoethyl methacrylate) were above 100 μmol/L. The concentrations to which the cells and tissues are exposed in uiuo are not known. This study should help to identify the concentrations of organic composite components which pose clinical cytotoxic hazards. | en_US |
dc.format.extent | 3108 bytes | |
dc.format.extent | 683255 bytes | |
dc.format.mimetype | text/plain | |
dc.format.mimetype | application/pdf | |
dc.publisher | SAGE Publications | en_US |
dc.title | Cytotoxic Effects of Resin Components on Cultured Mammalian Fibroblasts | en_US |
dc.type | Article | en_US |
dc.subject.hlbsecondlevel | Dentistry | en_US |
dc.subject.hlbtoplevel | Health Sciences | en_US |
dc.description.peerreviewed | Peer Reviewed | en_US |
dc.contributor.affiliationum | Departments of Oral Medicine, Pathology and Surgery, School of Dentistry, University of Michigan, Ann Arbor, Michigan 48109-1078 | en_US |
dc.contributor.affiliationum | Departments of Oral Medicine, Pathology and Surgery, School of Dentistry, University of Michigan, Ann Arbor, Michigan 48109-1078 | en_US |
dc.contributor.affiliationum | Biological and Materials Sciences, School of Dentistry, University of Michigan, Ann Arbor, Michigan 48109-1078 | en_US |
dc.contributor.affiliationum | Biological and Materials Sciences, School of Dentistry, University of Michigan, Ann Arbor, Michigan 48109-1078 | en_US |
dc.description.bitstreamurl | http://deepblue.lib.umich.edu/bitstream/2027.42/66623/2/10.1177_00220345910700111201.pdf | |
dc.identifier.doi | 10.1177/00220345910700111201 | en_US |
dc.identifier.citedreference | Baume, L.J. and Fiore-Donno, G. (1968): Response of the Human Pulp to a New Restorative Material, J Am Dent Assoc 76:1016-1022. | en_US |
dc.identifier.citedreference | Bergenholtz, G.; Cox, C.F.; Loesche, W.J.; and Syed, S.A. (1982): Bacterial Leakage Around Dental Restorations: Its Effect on the Dental Pulp, J Oral Pathol 11:439-450. | en_US |
dc.identifier.citedreference | Block, W.W.; Austin, J.C.; Cleaton-Jones, P.E.; Wilton-Cox, H.; and Fatti, L.P. (1977): Pulpal Response to a New Visible Light-cured Composite Restorative Material: Fotofil®, J Oral Pathol 6:278-287. | en_US |
dc.identifier.citedreference | Brannstrom, M. and Nyborg, H. (1972): Pulpal Reaction to Composite Resin Restorations, J Prosthet Dent 27:181-189. | en_US |
dc.identifier.citedreference | Cotran, R.S.; Kumar, V.; and Robbins, S.L. (1989): Pathologic Basis of Disease, Philadelphia: W.B. Saunders Co., pp. 1-38. | en_US |
dc.identifier.citedreference | Cox, C.F.; Keall, C.L.; Keall, H.J.; Ostro, E.; and Bergenholtz, G. (1987): Biocompatibility of Surface-sealed Dental Materials against Exposed Pulps, J Prosthet Dent 57:1-8. | en_US |
dc.identifier.citedreference | Darnell, J.; Lodish, H.; and Baltimore, D. (1986): Molecular Cell Biology, New York: W.H. Freeman and Company, pp. 105-129. | en_US |
dc.identifier.citedreference | Ferracane, J.L. and Condon, J.R. (1990): Rate of Elution of Leachable Components from Composite, Dent Mater 6:282-287. | en_US |
dc.identifier.citedreference | Freund, M. and Munksgaard, E.C. (1990): Enzymatic Degradation of Bis-GMA/TEGDMA Polymers Causing Decreased Microhardness and Greater Wear in vitro, Scand J Dent Res 98:351-355. | en_US |
dc.identifier.citedreference | Fujisawa, S.; Kadoma, Y.; and Komoda, Y. (1988): 1H and 13C NMR Studies of the Interaction of Eugenol, Phenol and Triethyleneglycol Dimethacrylate with Phospholipid Liposomes as a Model System for Odontoblast Membranes, J Dent Res 67:1438-1441. | en_US |
dc.identifier.citedreference | Gosselin, R.E.; Smith, R.P.; and Hodge, H.C. (1984): Clinical Toxicology of Commercial Products, 5th ed., Baltimore: Williams and Wilkins, p. 1-2. | en_US |
dc.identifier.citedreference | Guess, W.L.; Rosenbluth, S.A.; Schmidt, B.; and Autian, J. (1965): Agar Diffusion Method for Toxicity Screening of Plastics on Cultured Cell Monolayers, J Pharm Sci 54:1545-1547. | en_US |
dc.identifier.citedreference | Hanks, C.T.; Craig, R.G.; Diehl, M.L.; and Pashley, D.H. (1988): Cytotoxicity of Dental Composites and Other Materials in a New in vitro Device, J Oral Pathol 17:396-403. | en_US |
dc.identifier.citedreference | Hensten-Pettersen, A. and Helgeland, K. (1981): Sensitivity of Different Human Cell Lines in the Biologic Evaluation of Dental Resin-based Restorative Materials, Scand J Dent Res 89:102-107. | en_US |
dc.identifier.citedreference | Lu, F.C. (1985): Basic Toxicology: Fundamentals, Target Organs, and Risk Assessment, New York: Hemisphere Pub., p. 104. | en_US |
dc.identifier.citedreference | Nasjleti, C.E.; Castelli, W.A.; and Caffesse, R.G. (1983): Effects of Composite Restorations on the Periodontal Membrane of Monkeys, J Dent Res 62:75-78. | en_US |
dc.identifier.citedreference | Øysaed, H. and Ruyter, I.E. (1986): Water Sorption and Filler Characteristics of Composites for Use in Posterior Composites, J Dent Res 65:1315-1318. | en_US |
dc.identifier.citedreference | Rathbun, M.A.; Craig, R.G.; Hanks, C.T.; and Filisko, F.E. (1991): Cytotoxicity of a bis-GMA Dental Composite before and after Leaching in Organic Solvents, J Biomed Mater Res 25:443-457. | en_US |
dc.identifier.citedreference | Rueggeberg, F.A. and Craig, R.G. (1988): Correlation of Parameters Used to Estimate Monomer Conversion in a Light Cured Composite, J Dent Res 67:932-937. | en_US |
dc.identifier.citedreference | Ruyter, I.E. and Øysaed, H. (1987): Composites for Use in Posterior Teeth: Composition and Conversion, J Biomed Mater Res 21:11-23. | en_US |
dc.identifier.citedreference | Stanley, H.R.; Bowen, R.L.; and Folio, J. (1979): Compatibility of Various Materials with Oral Tissues. II. Responses to Composite Ingredients, J Dent Res 58:1507-1517. | en_US |
dc.identifier.citedreference | Stanley, H.R.; Going, R.E.; and Chauncey, H.H. (1975): Human Pulp Response to Acid Pretreatment of Dentin and to Composite Restoration, JAm Dent Assoc 91:817-825. | en_US |
dc.identifier.citedreference | Stanley, H.R.; Swerdlow, H.; and Buonocore, M.G. (1967): Pulp Reactions to Anterior Restorative Materials, J Am Dent Assoc 75 :132-141. | en_US |
dc.identifier.citedreference | Terakado, M.; Yamazaki, M.; Tsujimoto, Y.; Kawashima, T.; Nagashima, K. ; Ogawa, J.; Fujita, Y.; Sugiya, H.; Sakai, T.; and Furuyama, S. (1984): Lipid Peroxidation as a Possible Cause of Benzoyl Peroxide Toxicity in Rabbit Dental Pulp-a Microsomal Lipid Peroxidation in vitro, J Dent Res 63:901-905. | en_US |
dc.identifier.citedreference | Tobias, M.; Cataldo, E.; Shiere, F.R.; and Clark, R.E. (1973): Pulp Reaction to a Resin-bonded Quartz Composite Material, J Dent Res 52:1281-1286. | en_US |
dc.identifier.citedreference | Wennberg, A.; Hasselgren, G.; and Tronstad, L. (1979): A Method of Toxicity Screening of Biomaterials using Cells Cultured on Millipore Filters, J Biomed Mater Res 13:109-120. | en_US |
dc.owningcollname | Interdisciplinary and Peer-Reviewed |
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