Characterization of Cellular Responses Involved in Reparative Dentinogenesis in Rat Molars
dc.contributor.author | D'Souza, R. N. | en_US |
dc.contributor.author | Bachman, T. | en_US |
dc.contributor.author | Baumgardner, K. R. | en_US |
dc.contributor.author | Butler, W. T. | en_US |
dc.contributor.author | Litz, M. | en_US |
dc.date.accessioned | 2010-04-13T19:18:42Z | |
dc.date.available | 2010-04-13T19:18:42Z | |
dc.date.issued | 1995 | en_US |
dc.identifier.citation | D'Souza, R.N.; Bachman, T.; Baumgardner, K.R.; Butler, W.T.; Litz, M. (1995). "Characterization of Cellular Responses Involved in Reparative Dentinogenesis in Rat Molars." Journal of Dental Research 2(74): 702-709. <http://hdl.handle.net/2027.42/67273> | en_US |
dc.identifier.issn | 0022-0345 | en_US |
dc.identifier.uri | https://hdl.handle.net/2027.42/67273 | |
dc.description.abstract | During primary dentin formation, differentiating primary odontoblasts secrete an organic matrix, consisting principally of type I collagen and non-collagenous proteins, that is capable of mineralizing at its distal front. In contrast to ameloblasts that form enamel and undergo programed cell death, primary odontoblasts remain metabolically active in a functional tooth. When dentin is exposed to caries or by operative procedures, and when exposed dentinal tubules are treated with therapeutic dental materials, the original population of odontoblasts is often injured and destroyed. The characteristics of the replacement pool of cells that form reparative dentin and the biologic mechanisms that modulate the formation of this matrix are poorly understood. Based on the hypothesis that events governing primary dentinogenesis are reiterated during dentin repair, the present study was designed to test whether cells that form reparative dentin are odontoblast-like. Cervical cavities were prepared in rat first molars to generate reparative dentin, and animals were killed at various time intervals. In situ hybridization with gene-specific riboprobes for collagen types I and III was used to study de novo synthesis by cells at the injured dentin-pulp interface. Polyclonal antibodies raised against dentin sialoprotein (DSP), a dentin-specific protein that marks the odontoblast phenotype, were used in immunohistochemical experiments. Data from our temporal and spatial analyses indicated that cells forming reparative dentin synthesize type I but not type III collagen and are immunopositive for DSP. Our results suggest that cells that form reparative dentin are odontoblast-like. | en_US |
dc.format.extent | 3108 bytes | |
dc.format.extent | 1395938 bytes | |
dc.format.mimetype | text/plain | |
dc.format.mimetype | application/pdf | |
dc.publisher | SAGE Publications | en_US |
dc.subject.other | Odontoblasts | en_US |
dc.subject.other | Reparative Dentin | en_US |
dc.subject.other | Collagen | en_US |
dc.subject.other | Dentin Sialoprotein | en_US |
dc.subject.other | Dental Pulp | en_US |
dc.title | Characterization of Cellular Responses Involved in Reparative Dentinogenesis in Rat Molars | en_US |
dc.type | Article | en_US |
dc.subject.hlbsecondlevel | Dentistry | en_US |
dc.subject.hlbtoplevel | Health Sciences | en_US |
dc.description.peerreviewed | Peer Reviewed | en_US |
dc.contributor.affiliationum | Department of Cariology, Restorative Sciences and Endodontics, University of Michigan, Ann Arbor, Michigan | en_US |
dc.contributor.affiliationother | Department of Basic Sciences, Room 4.133 F | en_US |
dc.contributor.affiliationother | Division of Endodontics, Department of Stomatology, University of Texas at Houston Health Science Center, 6516 John Freeman Avenue, Houston, Texas 77030 | en_US |
dc.contributor.affiliationother | Department of Basic Sciences, Room 4.133 F | en_US |
dc.contributor.affiliationother | Department of Basic Sciences, Room 4.133 F | en_US |
dc.description.bitstreamurl | http://deepblue.lib.umich.edu/bitstream/2027.42/67273/2/10.1177_00220345950740021301.pdf | |
dc.identifier.doi | 10.1177/00220345950740021301 | en_US |
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dc.owningcollname | Interdisciplinary and Peer-Reviewed |
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