Effects of Unpolymerized Resin Components on the Function of Accessory Cells Derived from the Rat Incisor Pulp
dc.contributor.author | Jontell, M. | en_US |
dc.contributor.author | Hanks, Carl T. | en_US |
dc.contributor.author | Bratel, J. | en_US |
dc.contributor.author | Bergenholtz, G. | en_US |
dc.date.accessioned | 2010-04-13T19:38:47Z | |
dc.date.available | 2010-04-13T19:38:47Z | |
dc.date.issued | 1995 | en_US |
dc.identifier.citation | Jontell, M.; Hanks, C.T.; Bratel, J.; Bergenholtz, G. (1995). "Effects of Unpolymerized Resin Components on the Function of Accessory Cells Derived from the Rat Incisor Pulp." Journal of Dental Research 5(74): 1162-1167. <http://hdl.handle.net/2027.42/67618> | en_US |
dc.identifier.issn | 0022-0345 | en_US |
dc.identifier.uri | https://hdl.handle.net/2027.42/67618 | |
dc.description.abstract | Monomeric resin components from dental composites are toxic to fibroblasts in culture and thus may interfere with the local immune system of the pulp, reducing its effective defense potential, either by cytotoxicity or by a more specific immune mechanism. Therefore, the present study was undertaken to observe the cytotoxic effects elicited by certain unpolymerized components of resin composites upon the function of accessory pulp cells in mitogen-induced proliferation of T-lymphocytes. Accessory cells from the rat incisor pulp were released following enzymatic digestion with collagenase. The assay included incubation of these cells with purified T-lymphocytes from cervical lymph nodes for 72 h in the presence of different concentrations of the resin components. The proliferative T-lymphocyte response was monitored by 3H-thymidine incorporation. Initially, we conducted experiments on spleen cells to determine the proper concentration intervals for suitable testing of the resin components. To assess the individual susceptibility of accessory cells and T-lymphocytes, we pre-treated each of these cells with some of the test materials prior to assay. At low concentrations, urethane dimethacrylate (UDMA), bisglycidyl methacrylate (bis-GMA), triethylene glycol dimethacrylate (TEGDMA), and bis-phenol A (BPA) increased spleen cell proliferation to concanavalin A (con A). Purified T-lymphocytes stimulated by pulpal cells did not show enhanced responses to UDMA, bis-GMA, glycidyl methacrylate (GMA), or N,N-dihydroxyethyl-p-toluidine (DHEpT). At higher concentrations, all substances except camphoroquinone (CAMP) showed inhibitory effects in both test systems. The in vitro study shows that resin components can evoke either immunosuppression or immunostimulation on mitogen-driven proliferation of purified T-lymphocytes and spleen cells. | en_US |
dc.format.extent | 3108 bytes | |
dc.format.extent | 749432 bytes | |
dc.format.mimetype | text/plain | |
dc.format.mimetype | application/pdf | |
dc.publisher | SAGE Publications | en_US |
dc.subject.other | Dental Restoration | en_US |
dc.subject.other | Immunocompetence, | en_US |
dc.subject.other | Cytotoxicity | en_US |
dc.subject.other | Composites | en_US |
dc.subject.other | Lymphocytes | en_US |
dc.title | Effects of Unpolymerized Resin Components on the Function of Accessory Cells Derived from the Rat Incisor Pulp | en_US |
dc.type | Article | en_US |
dc.subject.hlbsecondlevel | Dentistry | en_US |
dc.subject.hlbtoplevel | Health Sciences | en_US |
dc.description.peerreviewed | Peer Reviewed | en_US |
dc.contributor.affiliationum | The Department of Oral Medicine, Pathology and Surgery, The University of Michigan School of Dentistry, Ann Arbor, Michigan, USA | en_US |
dc.contributor.affiliationother | Department of Endodontology and Oral Diagnosis, Facultyof Odontology, University of Goteborg, Goteborg, Sweden | en_US |
dc.contributor.affiliationother | Department of Endodontology and Oral Diagnosis, Facultyof Odontology, University of Goteborg, Goteborg, Sweden | en_US |
dc.contributor.affiliationother | Department of Endodontology and Oral Diagnosis, Facultyof Odontology, University of Goteborg, Goteborg, Sweden | en_US |
dc.description.bitstreamurl | http://deepblue.lib.umich.edu/bitstream/2027.42/67618/2/10.1177_00220345950740050401.pdf | |
dc.identifier.doi | 10.1177/00220345950740050401 | en_US |
dc.identifier.citedreference | Bergenholtz (1989). Bacterial leakage around dental restorations-impact on the pulp. Proceedings of symposium. In: Quality of dental restorations. Anusavice KJ, editor. Chicago: Quintessence Publishing Co., pp. 243-254. | en_US |
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dc.identifier.citedreference | Brännström M., Nyborg H. (1972). Pulpal reaction to composite resin restorations. J Prosthet Dent 27:181-189. | en_US |
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dc.identifier.citedreference | Ferracane JL, Condon JR (1990). Rate of elution of leachable components from composite. Dent Meter 6:282-287. Hanks CT, Strewn SE, Wataha JC, Craig RG (1991), Cytotoxic effects of resin components on cultured mammalian fibroblasts. J Dent Res 70:1450-1455. | en_US |
dc.identifier.citedreference | Jontell M., Bergenholtz G. (1992). Accessory cells in the immune defense of the dental pulp. Proc Finn Dent Soc 88(Suppl 1):344-355. | en_US |
dc.identifier.citedreference | Jontell M, Bergenholtz G, Scheynius A, Ambrose W (1988). Dendritic cells and macrophages expressing class II antigens in the normal rat incisor pulp. J Dent Res 67:1263-1266. Jontell M, Eklöf C, Dahlgren U, Bergenholtz C (1994). Difference in capacity between macrophages and dendritic cells from rat incisor pulp to provide signals to concanavalin A stimulated T lymphocytes. J Dent Res 73:1056-1060. Jontell M, Gunraj MN, Bergenholtz G (1987). Immunocompetent cells in the normal dental pulp. J Dent Res 66:1149-1153. Luster MI (1989). Immunotoxicology and the immune system. Health Environ Dig 3:1-3. | en_US |
dc.identifier.citedreference | Miller K., Anderson JM (1988). Human monocyte/macrophage activation and interleukin 1 generation by biomedical polymers. J Biomed Mater Res 22:713-731. | en_US |
dc.identifier.citedreference | Rathbun MA, Craig RG, Hanks CT, Filisko FE (1991). Cytotoxicity of a BIS-GMA dental composite before and after leaching in organic solvents. J Biomed Meter Res 25:443-457. | en_US |
dc.owningcollname | Interdisciplinary and Peer-Reviewed |
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