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Detection of Two Anaerobic Periodontopathogens in Children by Means of the BANA and ELISA Assays
dc.contributor.authorWatson, M.-R.en_US
dc.contributor.authorLopatin, Dennis E.en_US
dc.contributor.authorBretz, Walter A.en_US
dc.contributor.authorErtel, Inta J.en_US
dc.contributor.authorLoesche, Walter J.en_US
dc.date.accessioned2010-04-13T19:58:48Z
dc.date.available2010-04-13T19:58:48Z
dc.date.issued1991en_US
dc.identifier.citationWatson, M.-R.; Lopatin, D.E.; Bretz, W.A.; Ertel, I.J.; Loesche, W.J. (1991). "Detection of Two Anaerobic Periodontopathogens in Children by Means of the BANA and ELISA Assays." Journal of Dental Research 7(70): 1052-1056. <http://hdl.handle.net/2027.42/67961>en_US
dc.identifier.issn0022-0345en_US
dc.identifier.urihttps://hdl.handle.net/2027.42/67961
dc.description.abstractThe mouths of young children become colonized by a variety of bacteria, but there have been only a few studies that have sought the presence of periodontopathic species in this population. Almost all of these studies used culturing techniques rather than the newer detection methodologies for various periodontopathogens. Studies in adults have shown that Treponema denticola and Porphyromonas (Bacteroides) gingivalis can be detected in dental plaque by use of the BANA and ELISA diagnostic tests. In the present study, plaque samples from four subgingival sites in each of 157 children (aged from two to 18 years) were tested for BANA hydrolysis with a BANA reagent card, and for T. denticola and P. gingivalis with an ELISA assay. Anaerobic periodontopathogens hydrolyzing the BANA substrate were found to be present in at least one of four plaque samples in 88 children (56%). T. denticola and/or P. gingivalis were detected by ELISA in at least one plaque sample in each of 135 children (86%). This study shows that children are widely colonized by these micro-organisms. A higher proportion of Black children than Caucasian children was colonized by these BANA-positive organisms. Also, children having a parent with a documented history of periodontal disease were more likely to be BANA-positive than were children of parents with unknown periodontal status.en_US
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dc.publisherSAGE Publicationsen_US
dc.titleDetection of Two Anaerobic Periodontopathogens in Children by Means of the BANA and ELISA Assaysen_US
dc.typeArticleen_US
dc.subject.hlbsecondlevelDentistryen_US
dc.subject.hlbtoplevelHealth Sciencesen_US
dc.description.peerreviewedPeer Revieweden_US
dc.contributor.affiliationumDepartment of Biologic and Materials Sciences, The University of Michigan School of Dentistry, 1011 North University, Ann Arbor, MI 48109en_US
dc.contributor.affiliationumDepartment of Biologic and Materials Sciences, The University of Michigan School of Dentistry, 1011 North University, Ann Arbor, MI 48109en_US
dc.contributor.affiliationumDepartment of Biologic and Materials Sciences, The University of Michigan School of Dentistry, 1011 North University, Ann Arbor, MI 48109en_US
dc.contributor.affiliationumDepartment of Biologic and Materials Sciences, The University of Michigan School of Dentistry, 1011 North University, Ann Arbor, MI 48109en_US
dc.contributor.affiliationumDepartment of Biologic and Materials Sciences, The University of Michigan School of Dentistry, 1011 North University, Ann Arbor, MI 48109en_US
dc.description.bitstreamurlhttp://deepblue.lib.umich.edu/bitstream/2027.42/67961/2/10.1177_00220345910700070701.pdf
dc.identifier.doi10.1177/00220345910700070701en_US
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dc.owningcollnameInterdisciplinary and Peer-Reviewed


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