View Item 

Show simple item record

Biochemical and Morphological Studies of Rat Submandibular Gland: I. Centrifugal Fractionation of Granule-Rich Fraction
dc.contributor.authorHanks, Carl T.en_US
dc.contributor.authorChakrabarti, S. G.en_US
dc.date.accessioned2010-04-13T20:07:06Z
dc.date.available2010-04-13T20:07:06Z
dc.date.issued1975en_US
dc.identifier.citationHanks, C.T.; Chakrabarti, S.G. (1975). "Biochemical and Morphological Studies of Rat Submandibular Gland: I. Centrifugal Fractionation of Granule-Rich Fraction." Journal of Dental Research 5(54): 938-947. <http://hdl.handle.net/2027.42/68103>en_US
dc.identifier.issn0022-0345en_US
dc.identifier.urihttps://hdl.handle.net/2027.42/68103
dc.description.abstractSubmandibular glands from male rats were homogenized in 0.34 M sucrose and 0.5 mM ethylenediaminetetraacetic acid in 10 mM HEPES buffer at a pH of 7.4. The extract was centrifuged and filtered through nuclepore filters to prepare a granule-rich fraction. Electron dense zymogen granules constituted approximately 85% of the particles in this fraction which also contained about a third of the total alkaline esterase activity in the gland.en_US
dc.format.extent3108 bytes
dc.format.extent1199940 bytes
dc.format.mimetypetext/plain
dc.format.mimetypeapplication/pdf
dc.publisherSAGE Publicationsen_US
dc.titleBiochemical and Morphological Studies of Rat Submandibular Gland: I. Centrifugal Fractionation of Granule-Rich Fractionen_US
dc.typeArticleen_US
dc.subject.hlbsecondlevelDentistryen_US
dc.subject.hlbtoplevelHealth Sciencesen_US
dc.description.peerreviewedPeer Revieweden_US
dc.contributor.affiliationumInstitute of Dental Research, Departments of Oral Pathologyand Oral Biology, School of Dentistry, University of Michigan, Ann Arbor, Michigan 48104, USAen_US
dc.contributor.affiliationumInstitute of Dental Research, Departments of Oral Pathologyand Oral Biology, School of Dentistry, University of Michigan, Ann Arbor, Michigan 48104, USAen_US
dc.description.bitstreamurlhttp://deepblue.lib.umich.edu/bitstream/2027.42/68103/2/10.1177_00220345750540053201.pdf
dc.identifier.doi10.1177/00220345750540053201en_US
dc.identifier.citedreferenceBhoola, K.D.: Comparative Study of the Subcellular Distribution of Submaxillary Kallekrein, Biochem Pharmacol 18: 1252, 1960.en_US
dc.identifier.citedreferenceCohen, S.: Purification of a Nerve Growth Promoting Protein from the Mouse Salivary Gland and Its Neuro-Cytotoxic Antiserum, Proc Natl Acad Sci USA 46: 302, 1960.en_US
dc.identifier.citedreferenceErdös, E.G.; Tague, L.L.; and Miwa, I.: Kallekrein in Granules of the Submaxillary Gland, Biochem Pharmacol 17: 667, 1968.en_US
dc.identifier.citedreferenceGeipert, F., and Erdös, E.G.: Properties of Granules that Contain Kallekrein and Renin, Experientia 27: 912, 1971.en_US
dc.identifier.citedreferenceNaughton, M.A. ; Geczy, C.; Bender, V.; Hoffman, H.; and Hamilton, E.: Esteropeptidase and Thymotropic Activity of a Protein Isolated from Mouse Submaxillary Gland, Biochim Biophys Acta 263: 106, 1972.en_US
dc.identifier.citedreferenceChiang, T.S. ; Erdös, E.G.; Miwa, I.; Tague, L.; and Coalson, J.J.: Isolation from a Salivary Gland of Granules Containing Renin and Kallekrein, Circ Res 23: 507, 1968.en_US
dc.identifier.citedreferencePasquini, F. ; Petris, A.; Sbaraglia, G.; Sco-Pelliti, R.; Cenci, G.; and Frati, L.:. Biological Activities in the Granules Isolated from the Mouse Submaxillary Gland, Exp Cell Res 86: 233, 1974.en_US
dc.identifier.citedreferenceSreebny, L.M. : Studies of Salivary Gland Proteases, Ann NY Acad Sci 85: 182, 1960.en_US
dc.identifier.citedreferenceAngeletti, R.A. ; Angeletti, P.V.; and Cal-Issano, P.: Testosterone Induction of Estero-Proteolytic Activity in the Mouse Submaxillary Gland, Biochim Biophys Acta 139: 372, 1967.en_US
dc.identifier.citedreferenceRiekkinen, P.J., and Niemi, M.: Androgen-Dependent Salivary Gland Proteases in the Rat, Endocrinology 83: 1224, 1968.en_US
dc.identifier.citedreferenceShafer, W.G., and Muhler, J.C.: Endocrine Influences upon the Salivary Glands, Ann NY Acad Sci 85: 215, 1960.en_US
dc.identifier.citedreferenceSchwert, G.W., and Takenaka, Y.: A Spectrophotometric Determination of Trypsin and Chymotrypin, Biochim Biophys Acta 16: 570, 1955.en_US
dc.identifier.citedreferenceEkfors, T.O. ; Malmiharju, T.; and Hopsu-Havu, V.K.: Isolation of Six Trypsin-Like Esteropeptidases from the Mouse Submandibular Gland, Enzymology 43: 151, 1972.en_US
dc.identifier.citedreferenceHuggins, C., and Talalay, P.: Sodium Phenolphthalein Phosphate as a Substrate for Phosphatase Tests, J Biol Chem 159: 399, 1945.en_US
dc.identifier.citedreferenceNachlas, M.M. ; Margulis, S.I.; and Selig-Man, A.M.: Site of Electron Transfer to Tetrazolium Salts in the Succinoxidase System, J Biol Chem 235: 2739, 1960.en_US
dc.identifier.citedreferenceFiske, C.H., and Subbarow, Y.: The Colorimetric Determination of Phosphorus, J Biol Chem 66: 375, 1925.en_US
dc.identifier.citedreferenceLowry, O.H. ; Rosebrouch, N.J.; Farr, A.L.; and Randall, R.J.: Protein Measurement with Folin Phenol Reagent, J Biol Chem 193: 265, 1951.en_US
dc.identifier.citedreferenceLuft, J.H.: Improvements in Epoxy Resin Embedding Methods, J Biophys Biochem Cytol 9: 409, 1961.en_US
dc.identifier.citedreferenceMollenhauer, H.H.: Plastic Embedding Mixtures for Use in Electron Microscopy, Stain Technol 39: 111, 1964.en_US
dc.identifier.citedreferenceStempak, J.G., and Ward, R.T.: An Improved Staining Method for Electron Microscopy, J Cell Biol 22: 697, 1964.en_US
dc.identifier.citedreferenceReynolds, E.S. : The Use of Lead Citrate at High p H as an Electron-Opaque Stain in Electron Microscopy, J Cell Biol 17: 208, 1963.en_US
dc.identifier.citedreferenceHopsu, V.K., and Glenner, G.G.: A Histochemical Enzyme Kinetic System Applied to the Trypsin-Like Amidase and Esterase Activity in Human Mast Cells, J Cell Biol 17: 503, 1963.en_US
dc.identifier.citedreferenceAdams, C.W.M. : A p-Dimethylaminobenzaldehyde-nitrite Method for Histochemical Demonstration of Tryptophane and Related Compounds, J Clin Pathol 10: 56, 1957.en_US
dc.identifier.citedreferenceHanks, C.T., and Chaudhry, A.P.: Radio-autographic Evidence of 3H-Tryptophan Incorporation in Secretory Cells of Rat Submandibular Glands, J Dent Res 50: 1626, 1971.en_US
dc.identifier.citedreferenceTamarin, A. ; Wanamaker, B.; and Sreebny, L.M.: The Effect of Inanition of the Submandibular Salivary Glands and Exocrine Pancreas of the Rat, Ann NY Acad Sci 106: 609, 1963.en_US
dc.identifier.citedreferenceEkfors, T.O., and Hopsu-Havu, V.K.: Immuno-Fluorescent Localization of Trypsin-Like Esteropeptidases in the Mouse Submandibular Gland, Histochem J 3: 415, 1971.en_US
dc.identifier.citedreferenceRiekkinen, P.J.; Ekfors, T.O.; and Hopsu, V.K.: Purification and Characteristics of an Alkaline Protease from Rat Submandibular Gland, Biochim Biophys Acta 118: 604, 1966.en_US
dc.identifier.citedreferenceHopsu-HAvu, V.K.; Riekkinen, P.J.; and Ekfors, T.O.: Studies on the Alkaline Trypsin-Like Enzyme in Rat Submandibular Gland and Saliva, Acta Odontol Scand 25: 657, 1967.en_US
dc.owningcollnameInterdisciplinary and Peer-Reviewed


Files in this item

Name:
10.1177_00220345750540053201.pdf
Size:
1.144MB
Format:
PDF
View/Open

Show simple item record

Remediation of Harmful Language

The University of Michigan Library aims to describe library materials in a way that respects the people and communities who create, use, and are represented in our collections. Report harmful or offensive language in catalog records, finding aids, or elsewhere in our collections anonymously through our metadata feedback form. More information at Remediation of Harmful Language.

Accessibility

If you are unable to use this file in its current format, please select the Contact Us link and we can modify it to make it more accessible to you.