Fluorescence depolarization by anisotropic rotational diffusion of a luminophore and its carrier molecule
dc.contributor.author | Burghardt, Thomas P. | en_US |
dc.date.accessioned | 2010-05-06T21:34:29Z | |
dc.date.available | 2010-05-06T21:34:29Z | |
dc.date.issued | 1983-05-15 | en_US |
dc.identifier.citation | Burghardt, Thomas P. (1983). "Fluorescence depolarization by anisotropic rotational diffusion of a luminophore and its carrier molecule." The Journal of Chemical Physics 78(10): 5913-5919. <http://hdl.handle.net/2027.42/70062> | en_US |
dc.identifier.uri | https://hdl.handle.net/2027.42/70062 | |
dc.description.abstract | The analytical expression for the polarization anisotropy is derived for a luminophore undergoing rotational diffusion about a single axis while attached to a nonluminescing, rotationally diffusing, symmetrical carrier molecule. In contrast to previous related calculations, the rotation axis of the luminophore is assumed to have an arbitrary orientation relative to the carrier. Additionally, the polarization anisotropy is measured for bovine serum albumin (BSA) labeled with dansyl, NBD, rhodamine, or eosin that is: (a) surface adsorbed to a glass/buffer interface, using a variation of the technique of total internal reflection fluorescence spectroscopy (TIRFS), or (b) bulk dissolved, using conventional transmitted illumination fluorescence spectroscopy. With this theory, using previously published values for the rotational diffusion constants of BSA and the fluorescence lifetimes of the fluorophores, the rotational diffusion constant of the covalently bound probes is estimated from the measured anisotropy values. The results indicate a wide variability in the rotational diffusion constant of the probes (from ∼107 s−1 for dansyl to ∼109 s−1 for eosin) attached to both the surface adsorbed and bulk dissolved forms of BSA. Contrasting the rotational diffusion constant for each probe for surface adsorbed BSA vs bulk dissolved BSA indicates surface adsorption of the BSA molecule inhibits the rotational motion of the probe. These results have important implications in the application of other fluorescence techniques, such as singlet–singlet energy transfer, where the rotational mobility of the probe is important. | en_US |
dc.format.extent | 3102 bytes | |
dc.format.extent | 479909 bytes | |
dc.format.mimetype | text/plain | |
dc.format.mimetype | application/pdf | |
dc.publisher | The American Institute of Physics | en_US |
dc.rights | © The American Institute of Physics | en_US |
dc.title | Fluorescence depolarization by anisotropic rotational diffusion of a luminophore and its carrier molecule | en_US |
dc.type | Article | en_US |
dc.subject.hlbsecondlevel | Physics | en_US |
dc.subject.hlbtoplevel | Science | en_US |
dc.description.peerreviewed | Peer Reviewed | en_US |
dc.contributor.affiliationum | Department of Physics and Biophysics Research Division, University of Michigan, Ann Arbor, Michigan 48109 | en_US |
dc.description.bitstreamurl | http://deepblue.lib.umich.edu/bitstream/2027.42/70062/2/JCPSA6-78-10-5913-1.pdf | |
dc.identifier.doi | 10.1063/1.444596 | en_US |
dc.identifier.source | The Journal of Chemical Physics | en_US |
dc.identifier.citedreference | R. Rigler and M. Ehrenberg, Q. Rev. Biophys. 6, 139 (1973). | en_US |
dc.identifier.citedreference | D. C. Yang, W. E. Gall, and G. M. Edelman, J. Biol. Chem. 249 (21), 7018 (1974). | en_US |
dc.identifier.citedreference | J. Yguerabide, H. F. Epstein, and L. Stryer, J. Mol. Biol. 51, 573 (1970). | en_US |
dc.identifier.citedreference | J. R. Lakowicz and G. Weber, Biophys. J. 32, 591 (1980). | en_US |
dc.identifier.citedreference | W. F. Harrington, P. Johnson, and R. H. Ottewill, Biochem. J. 62, 562 (1956). | en_US |
dc.identifier.citedreference | P. Wahl and G. Weber, J. Mol. Biol. 30, 371 (1967). | en_US |
dc.identifier.citedreference | G. Lipari and A. Szabo, Biophys. J. 30, 489 (1980). | en_US |
dc.identifier.citedreference | I. Munro, I. Pecht, and L. Stryer, Proc. Natl. Acad. Sci. 76, 56 (1979). | en_US |
dc.identifier.citedreference | F. Jahnig, Proc. Natl. Acad. Sci. 76, 6361 (1979). | en_US |
dc.identifier.citedreference | K. Kinosita, Jr., S. Kawato, and A. Ikegami, Biophys. J. 20, 289 (1977). | en_US |
dc.identifier.citedreference | M. Shinitzky and P. Henkart, Int. Rev. Cytol. 60, 121 (1979). | en_US |
dc.identifier.citedreference | R. J. Cherry, FEBS Lett. 55, 1 (1975). | en_US |
dc.identifier.citedreference | R. H. Austin, S. S. Chan, and T. M. Jovin, Proc. Natl. Acad. Sci. 76 (11), 5650 (1979). | en_US |
dc.identifier.citedreference | D. Axelrod, Biophys. J. 26, 557 (1979). | en_US |
dc.identifier.citedreference | M. Shinitzky and Y. Barenholz, Biochim. Biophys. Acta 515, 367 (1978). | en_US |
dc.identifier.citedreference | Y. Gottlieb and P. Wahl, J. Chim. Phys. 60, 849 (1963). | en_US |
dc.identifier.citedreference | J. M. Schurr, Chem. Phys. 65, 417 (1982). | en_US |
dc.identifier.citedreference | S. Krause and C. T. O’Konski, J. Am. Chem. Soc. 81, 5082 (1959). | en_US |
dc.identifier.citedreference | T. P. Burghardt and D. Axelrod, Biochemistry 22, 979 (1983). | en_US |
dc.identifier.citedreference | L. D. Favro, Phys. Rev. 119, 53 (1960). | en_US |
dc.identifier.citedreference | L. D. Landau and E. M. Lifshitz, Quantum Mechanics: Nonrelativistic Theory (Pergamon, New York, 1975). | en_US |
dc.identifier.citedreference | A. S. Davydov, Quantum Mechanics (NEO, Ann Arbor, Michigan, 1966). | en_US |
dc.identifier.citedreference | N. J. Harrick, Internal Reflection Spectroscopy (Wiley, New York, 1966). | en_US |
dc.identifier.citedreference | T. P. Burghardt and D. Axelrod, Biophys. J. 33, 455 (1981). | en_US |
dc.identifier.citedreference | N. L. Thompson, Ph.D. thesis, University of Michigan, 1982. | en_US |
dc.identifier.citedreference | M. D. Barkley, A. A. Kowalczyk, and L. Brand, J. Chem. Phys. 75 (7), 3581 (1981). | en_US |
dc.identifier.citedreference | R. E. Dale and J. Eisinger, Biopolymers 13, 1573 (1974). | en_US |
dc.identifier.citedreference | CRC Standard Mathematical Tables, 16th ed., edited by S. M. Selby (Chemical Rubber, Clevland, Ohio, 1968). | en_US |
dc.identifier.citedreference | R. Haugland (private communication). | en_US |
dc.identifier.citedreference | H. E. Lessing and A. Von Jena, Chem. Phys. 41, 395 (1979). | en_US |
dc.owningcollname | Physics, Department of |
Files in this item
Remediation of Harmful Language
The University of Michigan Library aims to describe library materials in a way that respects the people and communities who create, use, and are represented in our collections. Report harmful or offensive language in catalog records, finding aids, or elsewhere in our collections anonymously through our metadata feedback form. More information at Remediation of Harmful Language.
Accessibility
If you are unable to use this file in its current format, please select the Contact Us link and we can modify it to make it more accessible to you.