The use of immunohistochemistry to distinguish reactive mesothelial cells from malignant mesothelioma in cytologic effusions
dc.contributor.author | Hasteh, Farnaz | en_US |
dc.contributor.author | Lin, Grace Y. | en_US |
dc.contributor.author | Weidner, Noel | en_US |
dc.contributor.author | Michael, Claire W. | en_US |
dc.date.accessioned | 2010-05-07T18:06:36Z | |
dc.date.available | 2011-03-01T16:26:45Z | en_US |
dc.date.issued | 2010-04-25 | en_US |
dc.identifier.citation | Hasteh, Farnaz; Lin, Grace Y.; Weidner, Noel; Michael, Claire W. (2010). "The use of immunohistochemistry to distinguish reactive mesothelial cells from malignant mesothelioma in cytologic effusions." Cancer Cytopathology 118(2): 90-96. <http://hdl.handle.net/2027.42/71361> | en_US |
dc.identifier.issn | 1934-662X | en_US |
dc.identifier.issn | 1934-6638 | en_US |
dc.identifier.uri | https://hdl.handle.net/2027.42/71361 | |
dc.description.abstract | BACKGROUND: The distinction of benign from malignant mesothelial proliferations in cytologic specimens can be problematic. In this study, the authors investigated the utility of immunohistochemical (IHC) markers in making this distinction. METHODS: Archival paraffin-embedded cell blocks of pleural and peritoneal fluids from 52 patients with malignant meothelioma (MM) and 64 patients with reactive mesothelial hyperplasia (MH) were retrieved. IHC stains included desmin, epithelial membrane antigen (EMA), glucose-transport protein 1 (GLUT-1), Ki67, and p53. RESULTS: Desmin was positive in 84% (54 of 64) cases of reactive MH and in 6% (3 of 52) of MM cases ( P < .001). EMA was positive in 9% (6 of 64) of benign and 100% (52 of 52) of malignant cases ( P < .001). GLUT-1 was positive in 12% (5 of 43) of benign and 47% (7 of 15) of malignant cases. Ki67 showed strong nuclear positivity in >40% of mesothelial cells in 9% (6 of 64) of benign and 16% (8 of 49) of malignant cases ( P = .38). p53 showed strong nuclear positivity in 2% (1 of 46) of benign and 47% (7 of 15) of malignant cases ( P < .001). EMA positivity and desmin negativity were found in 2% (1 of 64) of reactive MH cases and 98% (49 of 52) of MM cases ( P < .001). EMA negativity and desmin positivity were found in 86% (55 of 64) of reactive MH cases and 0% of MM cases. CONCLUSIONS: The combination of positive EMA and negative desmin strongly favors MM; conversely, a combination of negative EMA and positive desmin favors a reactive process. Likewise, strong membranous positivity for GLUT-1 and/or strong nuclear staining for p53 favors a mesothelioma. Ki67 proliferative index showed no significant difference between reactive MH and MM cases. Cancer (Cancer Cytopathol) 2010. © 2010 American Cancer Society. | en_US |
dc.format.extent | 279572 bytes | |
dc.format.extent | 3118 bytes | |
dc.format.mimetype | application/pdf | |
dc.format.mimetype | text/plain | |
dc.publisher | John Wiley & Sons, Inc. | en_US |
dc.subject.other | Life and Medical Sciences | en_US |
dc.subject.other | Cancer Research, Oncology and Pathology | en_US |
dc.title | The use of immunohistochemistry to distinguish reactive mesothelial cells from malignant mesothelioma in cytologic effusions | en_US |
dc.type | Article | en_US |
dc.rights.robots | IndexNoFollow | en_US |
dc.subject.hlbsecondlevel | Oncology and Hematology | en_US |
dc.subject.hlbtoplevel | Health Sciences | en_US |
dc.description.peerreviewed | Peer Reviewed | en_US |
dc.contributor.affiliationum | Department of Pathology, University of Michigan Medical Center, Ann Arbor, Michigan | en_US |
dc.contributor.affiliationother | Department of Pathology, University of California, San Diego, La Jolla, California ; Fax: (619) 543-5249 ; University of California, San Diego Medical Center, Department of Pathology, 200 W. Arbor Dr. MC 8720, San Diego, CA 92103-8720 | en_US |
dc.contributor.affiliationother | Department of Pathology, University of California, San Diego, La Jolla, California | en_US |
dc.contributor.affiliationother | Department of Pathology, University of California, San Diego, La Jolla, California | en_US |
dc.identifier.pmid | 20209622 | en_US |
dc.description.bitstreamurl | http://deepblue.lib.umich.edu/bitstream/2027.42/71361/1/20071_ftp.pdf | |
dc.identifier.doi | 10.1002/cncy.20071 | en_US |
dc.identifier.source | Cancer Cytopathology | en_US |
dc.owningcollname | Interdisciplinary and Peer-Reviewed |
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