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The use of immunohistochemistry to distinguish reactive mesothelial cells from malignant mesothelioma in cytologic effusions

dc.contributor.authorHasteh, Farnazen_US
dc.contributor.authorLin, Grace Y.en_US
dc.contributor.authorWeidner, Noelen_US
dc.contributor.authorMichael, Claire W.en_US
dc.date.accessioned2010-05-07T18:06:36Z
dc.date.available2011-03-01T16:26:45Zen_US
dc.date.issued2010-04-25en_US
dc.identifier.citationHasteh, Farnaz; Lin, Grace Y.; Weidner, Noel; Michael, Claire W. (2010). "The use of immunohistochemistry to distinguish reactive mesothelial cells from malignant mesothelioma in cytologic effusions." Cancer Cytopathology 118(2): 90-96. <http://hdl.handle.net/2027.42/71361>en_US
dc.identifier.issn1934-662Xen_US
dc.identifier.issn1934-6638en_US
dc.identifier.urihttps://hdl.handle.net/2027.42/71361
dc.description.abstractBACKGROUND: The distinction of benign from malignant mesothelial proliferations in cytologic specimens can be problematic. In this study, the authors investigated the utility of immunohistochemical (IHC) markers in making this distinction. METHODS: Archival paraffin-embedded cell blocks of pleural and peritoneal fluids from 52 patients with malignant meothelioma (MM) and 64 patients with reactive mesothelial hyperplasia (MH) were retrieved. IHC stains included desmin, epithelial membrane antigen (EMA), glucose-transport protein 1 (GLUT-1), Ki67, and p53. RESULTS: Desmin was positive in 84% (54 of 64) cases of reactive MH and in 6% (3 of 52) of MM cases ( P < .001). EMA was positive in 9% (6 of 64) of benign and 100% (52 of 52) of malignant cases ( P < .001). GLUT-1 was positive in 12% (5 of 43) of benign and 47% (7 of 15) of malignant cases. Ki67 showed strong nuclear positivity in >40% of mesothelial cells in 9% (6 of 64) of benign and 16% (8 of 49) of malignant cases ( P = .38). p53 showed strong nuclear positivity in 2% (1 of 46) of benign and 47% (7 of 15) of malignant cases ( P < .001). EMA positivity and desmin negativity were found in 2% (1 of 64) of reactive MH cases and 98% (49 of 52) of MM cases ( P < .001). EMA negativity and desmin positivity were found in 86% (55 of 64) of reactive MH cases and 0% of MM cases. CONCLUSIONS: The combination of positive EMA and negative desmin strongly favors MM; conversely, a combination of negative EMA and positive desmin favors a reactive process. Likewise, strong membranous positivity for GLUT-1 and/or strong nuclear staining for p53 favors a mesothelioma. Ki67 proliferative index showed no significant difference between reactive MH and MM cases. Cancer (Cancer Cytopathol) 2010. © 2010 American Cancer Society.en_US
dc.format.extent279572 bytes
dc.format.extent3118 bytes
dc.format.mimetypeapplication/pdf
dc.format.mimetypetext/plain
dc.publisherJohn Wiley & Sons, Inc.en_US
dc.subject.otherLife and Medical Sciencesen_US
dc.subject.otherCancer Research, Oncology and Pathologyen_US
dc.titleThe use of immunohistochemistry to distinguish reactive mesothelial cells from malignant mesothelioma in cytologic effusionsen_US
dc.typeArticleen_US
dc.rights.robotsIndexNoFollowen_US
dc.subject.hlbsecondlevelOncology and Hematologyen_US
dc.subject.hlbtoplevelHealth Sciencesen_US
dc.description.peerreviewedPeer Revieweden_US
dc.contributor.affiliationumDepartment of Pathology, University of Michigan Medical Center, Ann Arbor, Michiganen_US
dc.contributor.affiliationotherDepartment of Pathology, University of California, San Diego, La Jolla, California ; Fax: (619) 543-5249 ; University of California, San Diego Medical Center, Department of Pathology, 200 W. Arbor Dr. MC 8720, San Diego, CA 92103-8720en_US
dc.contributor.affiliationotherDepartment of Pathology, University of California, San Diego, La Jolla, Californiaen_US
dc.contributor.affiliationotherDepartment of Pathology, University of California, San Diego, La Jolla, Californiaen_US
dc.identifier.pmid20209622en_US
dc.description.bitstreamurlhttp://deepblue.lib.umich.edu/bitstream/2027.42/71361/1/20071_ftp.pdf
dc.identifier.doi10.1002/cncy.20071en_US
dc.identifier.sourceCancer Cytopathologyen_US
dc.owningcollnameInterdisciplinary and Peer-Reviewed


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