Effects of steady electric fields on human retinal pigment epithelial cell orientation and migration in culture
dc.contributor.author | Sulik, Gregory L. | en_US |
dc.contributor.author | Soong, H. Kaz | en_US |
dc.contributor.author | Chang, Patricia C. T. | en_US |
dc.contributor.author | Parkinson, William Charles | en_US |
dc.contributor.author | Elner, Susan G. | en_US |
dc.contributor.author | Elner, Victor M. | en_US |
dc.date.accessioned | 2010-06-01T18:11:39Z | |
dc.date.available | 2010-06-01T18:11:39Z | |
dc.date.issued | 1992-02 | en_US |
dc.identifier.citation | Sulik, Gregory L.; Soong, H. Kaz; Chang, Patricia C. T.; Parkinson, William C.; Elner, Susan G.; Elner, Victor M. (1992). "Effects of steady electric fields on human retinal pigment epithelial cell orientation and migration in culture." Acta Ophthalmologica 70(1): 115-122. <http://hdl.handle.net/2027.42/71405> | en_US |
dc.identifier.issn | 1755-375X | en_US |
dc.identifier.issn | 1755-3768 | en_US |
dc.identifier.uri | https://hdl.handle.net/2027.42/71405 | |
dc.description.abstract | Low-level, steady electric fields of 6–10 volts/cm stimulated directional orientation and translocation of cultured human retinal pigment epithelial cells. The orientative movements (galvanotropism) consisted of somatic elongation of the cells into spindle shapes, followed by pivotal alignment orthogonal to the field. The anodal edges of the cells underwent retraction of their plasmalemmal extensions, while the cathode edges and the longitudinal ends developed lamellipodia and ruffled membranes. These tropic movements were followed by a translocational movement (galvanotaxis) of the cells towards the cathode. Staining of these migrating cells for actin showed the accumulation of stress fibers at the leading (cathodal) edge, as well as at the longitudinal ends of the elongated somata. These results suggest that endogenous, biologically-generated electric fields (eg., injury currents) may play a role in the guidance and migration of retinal pigment epithelial cells after retinal injury. | en_US |
dc.format.extent | 768382 bytes | |
dc.format.extent | 3109 bytes | |
dc.format.mimetype | application/pdf | |
dc.format.mimetype | text/plain | |
dc.publisher | Blackwell Publishing Ltd | en_US |
dc.rights | 1992 Institution Acta Ophthalmologica Scandinavica | en_US |
dc.subject.other | Cell Migration | en_US |
dc.subject.other | Electric Field | en_US |
dc.subject.other | Retinal Pigment Epithelium | en_US |
dc.subject.other | Galvanotaxis | en_US |
dc.subject.other | Galvanotropism | en_US |
dc.title | Effects of steady electric fields on human retinal pigment epithelial cell orientation and migration in culture | en_US |
dc.type | Article | en_US |
dc.subject.hlbsecondlevel | Ophthalmology | en_US |
dc.subject.hlbtoplevel | Health Sciences | en_US |
dc.description.peerreviewed | Peer Reviewed | en_US |
dc.contributor.affiliationum | Departments of Ophthalmology, The University of Michigan, Ann Arbor, Michigan | en_US |
dc.contributor.affiliationum | Physics, The University of Michigan, Ann Arbor, Michigan | en_US |
dc.contributor.affiliationum | Pathology, The University of Michigan, Ann Arbor, Michigan | en_US |
dc.description.bitstreamurl | http://deepblue.lib.umich.edu/bitstream/2027.42/71405/1/j.1755-3768.1992.tb02102.x.pdf | |
dc.identifier.doi | 10.1111/j.1755-3768.1992.tb02102.x | en_US |
dc.identifier.source | Acta Ophthalmologica | en_US |
dc.identifier.citedreference | Anderson JD ( 1951 ): Galvanotaxis of slime mold. J Gen Physiol 35: 1 – 16. | en_US |
dc.identifier.citedreference | Chang PCT, Sulik GL, Soong HK, Parkinson WC, Qu X & Meyer RF ( 1991 ): Galvanotropic and galvanotaxic responses of corneal endothelial cells are inhibited by cytochalasin D and W-7. Invest Ophthalmol Vis Sci (suppl) 32: 1219. | en_US |
dc.identifier.citedreference | Cooper MS & Schliwa M ( 1986 ): Motility of cultured fish epidermal cells in the presence and absence of direct current electric fields. J Cell Biol 102: 1384 – 1399. | en_US |
dc.identifier.citedreference | Dineur E ( 1891 ): Note sur la sensibilite des leucocytes a l'electricite. Bull Seances Soc Belge Microscopie (Bruxelles) 18: 113 – 118. | en_US |
dc.identifier.citedreference | Elner SG, Strieter RM, Elner VM, Rollins BJ, Del Monte MA & Kunkel SL ( 1991 ): Monocyte chemotactic protein gene expression by cytokine-treated human retinal pigment epithelial cells. Lab Invest 64: 819 – 825. | en_US |
dc.identifier.citedreference | Erickson CA & Nuccitelli R ( 1984 ): Embryonic fibroblast motility and orientation can be influenced by physiological electric fields. J Cell Biol 98: 296 – 307. | en_US |
dc.identifier.citedreference | Ferrier J, Ross SM, Kanehisa J & Aubin JE ( 1986 ): Osteoclasts and osteoblasts migrate in opposite directions in response to a constant electric field. J Cell Physiol 129: 283 – 288. | en_US |
dc.identifier.citedreference | Goldman RD, Schloss JA & Starger JM ( 1976 ): Organizational changes of actin-like microfilaments during animal cell movement. In: Goldman RD, Pollard T & Rosenbaum J ( eds ). Cell Motility, p 217. Cold Spring Harb Press, Cold Spring Harbor ( ME ). | en_US |
dc.identifier.citedreference | Henderson D & Weber K ( 1979 ): Three-dimensional organization of microfilaments and microtubules in the cytoskeleton. Exp Cell Res 124: 301 – 309. | en_US |
dc.identifier.citedreference | Hinkle L, McCaig CD & Robinson KR ( 1981 ): The direction of growth of differentiating neurones and myoblasts from frog embryos in an applied electric field. J Physiol (London) 314: 121 – 135. | en_US |
dc.identifier.citedreference | Jaffe LD & Nuccitelli R ( 1974 ): An ultrasensitive vibrating electrode for measuring steady extracellular currents. J Cell Biol 63: 614 – 628. | en_US |
dc.identifier.citedreference | Jaffe LF & Stern CD ( 1979a ): Neurites grow faster towards the cathode than the anode in a steady field. J Exp Zool 209: 115 – 128. | en_US |
dc.identifier.citedreference | Jaffe LF & Stern CD ( 1979b ): Strong electrical currents leave the primitive streak of chick embryos. Science 206: 569 – 571. | en_US |
dc.identifier.citedreference | Lazarides E & Weber K ( 1974 ): Actin antibody: The specific visualization of actin filaments in non-muscle cells. Proc Natl Acad Sci USA 71: 2268 – 2272. | en_US |
dc.identifier.citedreference | Luther PW, Peng HB & Lin JJ ( 1983 ): Changes in cell shape and actin distribution induced by constant electric fields. Nature (London) 303: 61 – 64. | en_US |
dc.identifier.citedreference | McCaig CD & Robinson KR ( 1982 ): The ontogeny of the transepithelial potential difference in from embryos. Dev Biol 90: 335 – 339. | en_US |
dc.identifier.citedreference | Nuccitelli R ( 1938 ): Physiological electric fields can influence cell motility, growth, and polarity. In: Miller K ( ed ). Advances in Cell Biology. Vol 2, p 213. JAI Press, Greenwich. | en_US |
dc.identifier.citedreference | Nuccitelli R & Erickson CA ( 1983 ): Embryonic cell motility can be guided by physiological electric fields. Exp Cell Res 147: 195 – 201. | en_US |
dc.identifier.citedreference | Orida N & Feldman JD ( 1982 ): Directional protrusive pseudopodial activity and motility in macrophages induced by extracellular electric fields. Cell Motil Cytoskeleton 2: 243 – 255. | en_US |
dc.identifier.citedreference | Patel N & Poo M-m ( 1982 ): Orientation of neurite growth by extracellular electric fields. J Neurosci 2: 483 – 496. | en_US |
dc.identifier.citedreference | Robinson KR ( 1985 ): The responses of cells to electric fields: A review. J Cell Biol 101: 2023 – 2027. | en_US |
dc.identifier.citedreference | Robinson KR & Stump RF ( 1984 ): Self generated electrical currents through Xenopus neurulae. J Physiol 352: 339 – 352. | en_US |
dc.identifier.citedreference | Soong HK, Parkinson WC, Bafna S, Sulik GL & Huang S ( 1990a ): Movements of cultured corneal epithelial cells and stromal fibroblasts in electric fields. Invest Ophthalmol Vis Sci 31: 2278 – 2282. | en_US |
dc.identifier.citedreference | Soong HK, Parkinson WC, Sulik GL & Bafna S ( 1990b ): Effects of electric fields on cytoskeleton of corneal stromal fibroblasts. Curr Eye Res 9: 893 – 901. | en_US |
dc.identifier.citedreference | Stump RF & Robinson KR ( 1983 ): Xenopus neural crest cell migration in an applied electric field. J Cell Biol 97: 1226 – 1233. | en_US |
dc.identifier.citedreference | Trinkaus JP ( 1982 ): Some thoughts on directional cell movement during morphogenesis. In: Bellairs R, Curtis A & Dunn G ( eds ). Cell Behaviour, p 471. Cambridge University Press, Cambridge. | en_US |
dc.identifier.citedreference | Verwon M ( 1896 ): Untersuchungen Über die polare Erregung der lebendigen Substanz durch den konstanten Strom. III. Mitteliung. Pflugers Arch Eur J Physiol 62: 415 – 450. | en_US |
dc.identifier.citedreference | Wehland J, Osborn M & Weber K ( 1984 ): Cell to substate contacts in living cells, a direct correlation between interference reflexion and indirect immunofluorescent microscopy using antibodies against actin and neurulae. J Physiol 352: 339 – 352. | en_US |
dc.identifier.citedreference | Yang WP, Onuma EK & Hui SW ( 1984 ): Response of C3H/10T1/2 fibroblasts to an external steady electric field stimulation: Reorientation, shape change, Con A receptor and intramembranous particle distibution and cytoskeleton reorganization. Exp Cell Res 155: 91 – 104. | en_US |
dc.owningcollname | Interdisciplinary and Peer-Reviewed |
Files in this item
Remediation of Harmful Language
The University of Michigan Library aims to describe library materials in a way that respects the people and communities who create, use, and are represented in our collections. Report harmful or offensive language in catalog records, finding aids, or elsewhere in our collections anonymously through our metadata feedback form. More information at Remediation of Harmful Language.
Accessibility
If you are unable to use this file in its current format, please select the Contact Us link and we can modify it to make it more accessible to you.