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Effects of steady electric fields on human retinal pigment epithelial cell orientation and migration in culture

dc.contributor.authorSulik, Gregory L.en_US
dc.contributor.authorSoong, H. Kazen_US
dc.contributor.authorChang, Patricia C. T.en_US
dc.contributor.authorParkinson, William Charlesen_US
dc.contributor.authorElner, Susan G.en_US
dc.contributor.authorElner, Victor M.en_US
dc.date.accessioned2010-06-01T18:11:39Z
dc.date.available2010-06-01T18:11:39Z
dc.date.issued1992-02en_US
dc.identifier.citationSulik, Gregory L.; Soong, H. Kaz; Chang, Patricia C. T.; Parkinson, William C.; Elner, Susan G.; Elner, Victor M. (1992). "Effects of steady electric fields on human retinal pigment epithelial cell orientation and migration in culture." Acta Ophthalmologica 70(1): 115-122. <http://hdl.handle.net/2027.42/71405>en_US
dc.identifier.issn1755-375Xen_US
dc.identifier.issn1755-3768en_US
dc.identifier.urihttps://hdl.handle.net/2027.42/71405
dc.description.abstractLow-level, steady electric fields of 6–10 volts/cm stimulated directional orientation and translocation of cultured human retinal pigment epithelial cells. The orientative movements (galvanotropism) consisted of somatic elongation of the cells into spindle shapes, followed by pivotal alignment orthogonal to the field. The anodal edges of the cells underwent retraction of their plasmalemmal extensions, while the cathode edges and the longitudinal ends developed lamellipodia and ruffled membranes. These tropic movements were followed by a translocational movement (galvanotaxis) of the cells towards the cathode. Staining of these migrating cells for actin showed the accumulation of stress fibers at the leading (cathodal) edge, as well as at the longitudinal ends of the elongated somata. These results suggest that endogenous, biologically-generated electric fields (eg., injury currents) may play a role in the guidance and migration of retinal pigment epithelial cells after retinal injury.en_US
dc.format.extent768382 bytes
dc.format.extent3109 bytes
dc.format.mimetypeapplication/pdf
dc.format.mimetypetext/plain
dc.publisherBlackwell Publishing Ltden_US
dc.rights1992 Institution Acta Ophthalmologica Scandinavicaen_US
dc.subject.otherCell Migrationen_US
dc.subject.otherElectric Fielden_US
dc.subject.otherRetinal Pigment Epitheliumen_US
dc.subject.otherGalvanotaxisen_US
dc.subject.otherGalvanotropismen_US
dc.titleEffects of steady electric fields on human retinal pigment epithelial cell orientation and migration in cultureen_US
dc.typeArticleen_US
dc.subject.hlbsecondlevelOphthalmologyen_US
dc.subject.hlbtoplevelHealth Sciencesen_US
dc.description.peerreviewedPeer Revieweden_US
dc.contributor.affiliationumDepartments of Ophthalmology, The University of Michigan, Ann Arbor, Michiganen_US
dc.contributor.affiliationumPhysics, The University of Michigan, Ann Arbor, Michiganen_US
dc.contributor.affiliationumPathology, The University of Michigan, Ann Arbor, Michiganen_US
dc.description.bitstreamurlhttp://deepblue.lib.umich.edu/bitstream/2027.42/71405/1/j.1755-3768.1992.tb02102.x.pdf
dc.identifier.doi10.1111/j.1755-3768.1992.tb02102.xen_US
dc.identifier.sourceActa Ophthalmologicaen_US
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dc.owningcollnameInterdisciplinary and Peer-Reviewed


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