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Altered growth characteristics of skin fibroblasts from wild-derived mice, and genetic loci regulating fibroblast clone size
dc.contributor.authorYuan, Rongen_US
dc.contributor.authorFlurkey, Kevinen_US
dc.contributor.authorVan Aelst-Bouma, Reneeen_US
dc.contributor.authorZhang, Weidongen_US
dc.contributor.authorKing, Benjaminen_US
dc.contributor.authorAustad, Steveen_US
dc.contributor.authorMiller, Richard A.en_US
dc.contributor.authorHarrison, David E.en_US
dc.date.accessioned2010-06-01T18:12:19Z
dc.date.available2010-06-01T18:12:19Z
dc.date.issued2006-06en_US
dc.identifier.citationYuan, Rong; Flurkey, Kevin; Van Aelst-Bouma, Renee; Zhang, Weidong; King, Benjamin; Austad, Steve; Miller, Richard A.; Harrison, David E. (2006). "Altered growth characteristics of skin fibroblasts from wild-derived mice, and genetic loci regulating fibroblast clone size." Aging Cell 5(3): 203-212. <http://hdl.handle.net/2027.42/71416>en_US
dc.identifier.issn1474-9718en_US
dc.identifier.issn1474-9726en_US
dc.identifier.urihttps://hdl.handle.net/2027.42/71416
dc.identifier.urihttp://www.ncbi.nlm.nih.gov/sites/entrez?cmd=retrieve&db=pubmed&list_uids=16842493&dopt=citationen_US
dc.description.abstractMouse fibroblast senescence in vitro is an important model for the study of aging at cellular level. However, common laboratory mouse strains may have lost some important allele variations related to aging processes. In this study, growth in vitro of tail skin fibroblasts (TSFs) derived from a wild-derived stock, Pohnpei (Pohn) mice, differed from growth of control C57BL/6 J (B6) TSFs. Pohn TSFs exhibited higher proliferative ability, fewer apoptotic cells, decreased expression of Cip1 , smaller surface areas, fewer cells positive for senescence associated-β-galactosidase (SA-β-gal) and greater resistance to H 2 O 2 -induced SA-β-gal staining and Cip1 expression. These data suggest that TSFs from Pohn mice resist cellular senescence-like changes. Using large clone ratio (LCR) as the phenotype, a quantitative trait locus (QTL) analysis in a Pohn/B6 backcross population found four QTLs for LCR: Fcs1 on Chr 3 at 55 cm; Fcs2 on Chr X at 50 cm; Fcs3 on Chr 4 at 51 cm and Fcs4 on Chr 10 at 25 cm. Together, these four QTLs explain 26.1% of the variations in LCRs in the N2 population. These are the first QTLs reported that regulate fibroblast growth. Glutathione S transferase mu ( GST-mu ) genes are overrepresented in the 95% confidence interval of Fcs1 , and Pohn TSFs have higher H 2 O 2 -induced GST-mu 4 , 5 and 7 mRNA levels than B6 TSFs. These enzymes may protect Pohn TSFs from oxidation.en_US
dc.format.extent241039 bytes
dc.format.extent3109 bytes
dc.format.mimetypeapplication/pdf
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dc.publisherBlackwell Publishing Ltden_US
dc.rights© 2006 The Authors Journal compilation © Blackwell Publishing Ltd/Anatomical Society of Great Britain and Ireland 2006en_US
dc.subject.otherFibroblasten_US
dc.subject.otherOxidative Stress (OS)en_US
dc.subject.otherQuantitative Trait Locus (QTL)en_US
dc.titleAltered growth characteristics of skin fibroblasts from wild-derived mice, and genetic loci regulating fibroblast clone sizeen_US
dc.typeArticleen_US
dc.subject.hlbsecondlevelMolecular, Cellular and Developmental Biologyen_US
dc.subject.hlbtoplevelHealth Sciencesen_US
dc.description.peerreviewedPeer Revieweden_US
dc.contributor.affiliationumDepartment of Pathology, Geriatrics Center, and VA GRECC, University of Michigan School of Medicine, 5316 CCGCB, Box 0940, 1500 E. Medical Center Drive, Ann Arbor, MI 48109, USAen_US
dc.contributor.affiliationotherThe Jackson Laboratory, 600 Main Street, Bar Harbor, ME 04609, USAen_US
dc.contributor.affiliationotherDepartment of Cellular & Structural Biology, Barshop Institute for Longevity & Aging Studies, University of Texas Health Science Center, 15355 Lambda Drive, STCBM Bldg. 3.100, San Antonio, TX 78245, USAen_US
dc.identifier.pmid16842493en_US
dc.description.bitstreamurlhttp://deepblue.lib.umich.edu/bitstream/2027.42/71416/1/j.1474-9726.2006.00208.x.pdf
dc.identifier.doi10.1111/j.1474-9726.2006.00208.xen_US
dc.identifier.sourceAging Cellen_US
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dc.owningcollnameInterdisciplinary and Peer-Reviewed


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