Ia ANTIGENS ON MURINE LYMPHOID CELLS: DISTRIBUTION, SURFACE MOVEMENT AND PARTIAL CHARACTERIZATION *
dc.contributor.author | Goding, J. W. | en_US |
dc.contributor.author | Nossal, G. J. V. | en_US |
dc.contributor.author | Shreffler, Donald C. | en_US |
dc.contributor.author | Marchalonis, J. J. | en_US |
dc.date.accessioned | 2010-06-01T18:18:22Z | |
dc.date.available | 2010-06-01T18:18:22Z | |
dc.date.issued | 1975-02 | en_US |
dc.identifier.citation | Goding, J. W.; Nossal, G. J. V.; Shreffler, D. C.; Marchalonis, J. J. (1975). "Ia ANTIGENS ON MURINE LYMPHOID CELLS: DISTRIBUTION, SURFACE MOVEMENT AND PARTIAL CHARACTERIZATION * ." International Journal of Immunogenetics 2(1): 9-25. <http://hdl.handle.net/2027.42/71516> | en_US |
dc.identifier.issn | 1744-3121 | en_US |
dc.identifier.issn | 1744-313X | en_US |
dc.identifier.uri | https://hdl.handle.net/2027.42/71516 | |
dc.description.abstract | Antisera directed against the I (immune response gene) segment of the murine major histocompatibility complex were prepared, and the distribution of lymphoid cells binding the antisera studied by radioautography. A clear bimodal distribution of grain counts was observed in lymph node and spleen. Bone marrow lymphocytes showed heavy but heterogeneous labelling, while thymocytes were lightly but definitely labelled. Approximately 30% of lymph node cells and 60% of spleen cells were heavily labelled. The heavily labelled cells were shown to be B cells. Ia antigens are present on plaque forming cells, but probably in smaller amounts than on resting B cells. One plasma cell tumour was studied, and was found to possess little or no Ia antigen. There was no demonstrable labelling of thoracic duct lymphocytes from preparations of T cells activated to histocompatibility antigens. However, several T cell lymphomas probably possess Ia antigens, since they were rejected when transplanted into mice differing at the I region. Overall, the data indicate that Ia antigens are expressed on at least some T cells. The Ia antigens were shown to move independently of surface immunoglobulin on B cells, demonstrating the non-identity of these molecules. Pretreatment of B cells with anti-immunoglobulin did not inhibit binding of anti-Ia sera. The Ia antigens could be capped by a sandwich technique in 75% of labelled cells. The Ia antigens were specifically precipitated from lysates of surface radioiodinated spleen cells, and the products analysed by polyacrylamide gel electrophoresis in the presence of sodium dodecyl sulphate. A single specific peak of radioactivity corresponding to a molecular weight of approximately 30,000 was found. The significance of these results in relation to immune response genes, receptors for antigen on T cells, and receptors for the Fc portion of IgG on B cells, is discussed. | en_US |
dc.format.extent | 1097287 bytes | |
dc.format.extent | 3109 bytes | |
dc.format.mimetype | application/pdf | |
dc.format.mimetype | text/plain | |
dc.publisher | Blackwell Publishing Ltd | en_US |
dc.rights | 1975 Blackwell Scientific Publications | en_US |
dc.title | Ia ANTIGENS ON MURINE LYMPHOID CELLS: DISTRIBUTION, SURFACE MOVEMENT AND PARTIAL CHARACTERIZATION * | en_US |
dc.type | Article | en_US |
dc.subject.hlbsecondlevel | Microbiology and Immunology | en_US |
dc.subject.hlbtoplevel | Health Sciences | en_US |
dc.description.peerreviewed | Peer Reviewed | en_US |
dc.contributor.affiliationum | † Hospital, Parkville 3050, Victoria, Australia and the Department of Human Genetics University of Michigan, Ann Arbor, Michigan | en_US |
dc.contributor.affiliationother | The Walter and Eliza Hall Institute of Medical Research, Post Office, Royal Melbourne | en_US |
dc.description.bitstreamurl | http://deepblue.lib.umich.edu/bitstream/2027.42/71516/1/j.1744-313X.1975.tb00500.x.pdf | |
dc.identifier.doi | 10.1111/j.1744-313X.1975.tb00500.x | en_US |
dc.identifier.source | International Journal of Immunogenetics | en_US |
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dc.owningcollname | Interdisciplinary and Peer-Reviewed |
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