Repression of motility during fimbrial expression: identification of 14 mrpJ gene paralogues in Proteus mirabilis
dc.contributor.author | Pearson, Melanie M. | en_US |
dc.contributor.author | Mobley, Harry L. T. | en_US |
dc.date.accessioned | 2010-06-01T18:19:36Z | |
dc.date.available | 2010-06-01T18:19:36Z | |
dc.date.issued | 2008-07 | en_US |
dc.identifier.citation | Pearson, Melanie M.; Mobley, Harry L. T. (2008). "Repression of motility during fimbrial expression: identification of 14 mrpJ gene paralogues in Proteus mirabilis ." Molecular Microbiology 69(2): 548-558. <http://hdl.handle.net/2027.42/71536> | en_US |
dc.identifier.issn | 0950-382X | en_US |
dc.identifier.issn | 1365-2958 | en_US |
dc.identifier.uri | https://hdl.handle.net/2027.42/71536 | |
dc.identifier.uri | http://www.ncbi.nlm.nih.gov/sites/entrez?cmd=retrieve&db=pubmed&list_uids=18630347&dopt=citation | en_US |
dc.description.abstract | Proteus mirabilis alternates between motile and adherent forms. MrpJ, a transcriptional regulator previously reported to repress motility, is encoded at the 3′ end of the mrp fimbrial operon in P. mirabilis . Sequencing of the P. mirabilis genome revealed 14 additional paralogues of mrpJ , 10 of which are associated with fimbrial operons. Twelve of these genes, when overexpressed, repressed motility; several distinct patterns of swarming motility were noted. Expression of 10 of the 14 mrpJ paralogues repressed flagellin (FlaA) synthesis. Alignment of the predicted amino acid sequences of MrpJ and its 14 paralogues revealed a conserved consensus motif (SQQQFSRYE) within the helix-turn-helix domain. Site-directed mutagenesis of these residues coupled with linker insertion mutagenesis of MrpJ confirmed the importance of this domain for repression of motility. Gel shift assays demonstrated that MrpJ and another paralogue UcaJ bind directly to the promoter region of the flagellar master regulator flhDC . Thus, P. mirabilis appears to use a related mechanism to inhibit motility during the production of at least 10 of its predicted fimbriae. | en_US |
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dc.format.mimetype | application/pdf | |
dc.format.mimetype | application/pdf | |
dc.format.mimetype | text/plain | |
dc.publisher | Blackwell Publishing Ltd | en_US |
dc.rights | Journal compilation © 2008 Blackwell Publishing | en_US |
dc.title | Repression of motility during fimbrial expression: identification of 14 mrpJ gene paralogues in Proteus mirabilis | en_US |
dc.type | Article | en_US |
dc.subject.hlbsecondlevel | Microbiology and Immunology | en_US |
dc.subject.hlbtoplevel | Science | en_US |
dc.description.peerreviewed | Peer Reviewed | en_US |
dc.identifier.pmid | 18630347 | en_US |
dc.description.bitstreamurl | http://deepblue.lib.umich.edu/bitstream/2027.42/71536/1/j.1365-2958.2008.06307.x.pdf | |
dc.description.bitstreamurl | http://deepblue.lib.umich.edu/bitstream/2027.42/71536/2/MMI_6307_sm_Figure_S1_and_Tables_S1-S3.pdf | |
dc.identifier.doi | 10.1111/j.1365-2958.2008.06307.x | en_US |
dc.identifier.source | Molecular Microbiology | en_US |
dc.identifier.citedreference | Adegbola, R.A., Old, D.C., and Senior, B.W. ( 1983 ) The adhesins and fimbriae of Proteus mirabilis strains associated with high and low affinity for the urinary tract. J Med Microbiol 16: 427 – 431. | en_US |
dc.identifier.citedreference | Akerley, B.J., Cotter, P.A., and Miller, J.F. ( 1995 ) Ectopic expression of the flagellar regulon alters development of the Bordetella –host interaction. Cell 80: 611 – 620. | en_US |
dc.identifier.citedreference | Bahrani, F.K., and Mobley, H.L. ( 1993 ) Proteus mirabilis MR/P fimbriae: molecular cloning, expression, and nucleotide sequence of the major fimbrial subunit gene. J Bacteriol 175: 457 – 464. | en_US |
dc.identifier.citedreference | Bahrani, F.K., Johnson, D.E., Robbins, D., and Mobley, H.L. ( 1991 ) Proteus mirabilis flagella and MR/P fimbriae: isolation, purification, N-terminal analysis, and serum antibody response following experimental urinary tract infection. Infect Immun 59: 3574 – 3580. | en_US |
dc.identifier.citedreference | Bahrani, F.K., Cook, S., Hull, R.A., Massad, G., and Mobley, H.L. ( 1993 ) Proteus mirabilis fimbriae: N-terminal amino acid sequence of a major fimbrial subunit and nucleotide sequences of the genes from two strains. Infect Immun 61: 884 – 891. | en_US |
dc.identifier.citedreference | Bahrani, F.K., Massad, G., Lockatell, C.V., Johnson, D.E., Russell, R.G., Warren, J.W., and Mobley, H.L. ( 1994 ) Construction of an MR/P fimbrial mutant of Proteus mirabilis: role in virulence in a mouse model of ascending urinary tract infection. Infect Immun 62: 3363 – 3371. | en_US |
dc.identifier.citedreference | Bijlsma, I.G., van Dijk, L., Kusters, J.G., and Gaastra, W. ( 1995 ) Nucleotide sequences of two fimbrial major subunit genes, pmpA and ucaA, from canine-uropathogenic Proteus mirabilis strains. Microbiology 141 ( Part 6 ): 1349 – 1357. | en_US |
dc.identifier.citedreference | Bryan, A., Roesch, P., Davis, L., Moritz, R., Pellett, S., and Welch, R.A. ( 2006 ) Regulation of type 1 fimbriae by unlinked FimB- and FimE-like recombinases in uropathogenic Escherichia coli strain CFT073. Infect Immun 74: 1072 – 1083. | en_US |
dc.identifier.citedreference | Clegg, S., and Hughes, K.T. ( 2002 ) FimZ is a molecular link between sticking and swimming in Salmonella enterica serovar Typhimurium. J Bacteriol 184: 1209 – 1213. | en_US |
dc.identifier.citedreference | Coker, C., Poore, C.A., Li, X., and Mobley, H.L. ( 2000 ) Pathogenesis of Proteus mirabilis urinary tract infection. Microbes Infect 2: 1497 – 1505. | en_US |
dc.identifier.citedreference | Cook, S.W., Mody, N., Valle, J., and Hull, R. ( 1995 ) Molecular cloning of Proteus mirabilis uroepithelial cell adherence ( uca ) genes. Infect Immun 63: 2082 – 2086. | en_US |
dc.identifier.citedreference | Finlay, B.B., and Falkow, S. ( 1997 ) Common themes in microbial pathogenicity revisited. Microbiol Mol Biol Rev 61: 136 – 169. | en_US |
dc.identifier.citedreference | Gardel, C.L., and Mekalanos, J.J. ( 1996 ) Alterations in Vibrio cholerae motility phenotypes correlate with changes in virulence factor expression. Infect Immun 64: 2246 – 2255. | en_US |
dc.identifier.citedreference | He, H., Snyder, H.A., and Forst, S. ( 2004 ) Unique organization and regulation of the mrx fimbrial operon in Xenorhabdus nematophila. Microbiology 150: 1439 – 1446. | en_US |
dc.identifier.citedreference | Heimer, S.R., and Mobley, H.L. ( 2001 ) Interaction of Proteus mirabilis urease apoenzyme and accessory proteins identified with yeast two-hybrid technology. J Bacteriol 183: 1423 – 1433. | en_US |
dc.identifier.citedreference | Jansen, A.M., Lockatell, C.V., Johnson, D.E., and Mobley, H.L. ( 2003 ) Visualization of Proteus mirabilis morphotypes in the urinary tract: the elongated swarmer cell is rarely observed in ascending urinary tract infection. Infect Immun 71: 3607 – 3613. | en_US |
dc.identifier.citedreference | Lane, M.C., Simms, A.N., and Mobley, H.L. ( 2007 ) Complex interplay between type 1 fimbrial expression and flagellum-mediated motility of uropathogenic Escherichia coli. J Bacteriol 189: 5523 – 5533. | en_US |
dc.identifier.citedreference | Latta, R.K., Grondin, A., Jarrell, H.C., Nicholls, G.R., and BÉrubÉ, L.R. ( 1999 ) Differential expression of nonagglutinating fimbriae and MR/P pili in swarming colonies of Proteus mirabilis. J Bacteriol 181: 3220 – 3225. | en_US |
dc.identifier.citedreference | Li, X., Rasko, D.A., Lockatell, C.V., Johnson, D.E., and Mobley, H.L. ( 2001 ) Repression of bacterial motility by a novel fimbrial gene product. EMBO J 20: 4854 – 4862. | en_US |
dc.identifier.citedreference | Li, X., Lockatell, C.V., Johnson, D.E., and Mobley, H.L. ( 2002 ) Identification of MrpI as the sole recombinase that regulates the phase variation of MR/P fimbria, a bladder colonization factor of uropathogenic Proteus mirabilis. Mol Microbiol 45: 865 – 874. | en_US |
dc.identifier.citedreference | Livak, K.J., and Schmittgen, T.D. ( 2001 ) Analysis of relative gene expression data using real-time quantitative PCR and the 2(-Delta Delta C(T)) Method. Methods 25: 402. | en_US |
dc.identifier.citedreference | Macnab, R.M. ( 1996 ) Flagella and motility. In Escherichia coli and Salmonella: Cellular and Molecular Biology. Neidhardt, F.C. (ed.). Washington, DC: American Society for Microbiology Press, pp. 123 – 145. | en_US |
dc.identifier.citedreference | Massad, G., Lockatell, C.V., Johnson, D.E., and Mobley, H.L. ( 1994a ) Proteus mirabilis fimbriae: construction of an isogenic pmfA mutant and analysis of virulence in a CBA mouse model of ascending urinary tract infection. Infect Immun 62: 536 – 542. | en_US |
dc.identifier.citedreference | Massad, G., Bahrani, F.K., and Mobley, H.L. ( 1994b ) Proteus mirabilis fimbriae: identification, isolation, and characterization of a new ambient-temperature fimbria. Infect Immun 62: 1989 – 1994. | en_US |
dc.identifier.citedreference | Meslet-Cladiere, L.M., Pimenta, A., Duchaud, E., Holland, I.B., and Blight, M.A. ( 2004 ) In vivo expression of the mannose-resistant fimbriae of Photorhabdus temperata K122 during insect infection. J Bacteriol 186: 611 – 622. | en_US |
dc.identifier.citedreference | Mobley, H.L., and Warren, J.W. ( 1987 ) Urease-positive bacteriuria and obstruction of long-term urinary catheters. J Clin Microbiol 25: 2216 – 2217. | en_US |
dc.identifier.citedreference | Mobley, H.L., Belas, R., Lockatell, V., Chippendale, G., Trifillis, A.L., Johnson, D.E., and Warren, J.W. ( 1996 ) Construction of a flagellum-negative mutant of Proteus mirabilis: effect on internalization by human renal epithelial cells and virulence in a mouse model of ascending urinary tract infection. Infect Immun 64: 5332 – 5340. | en_US |
dc.identifier.citedreference | Pearson, M.M., Sebaihia, M., Churcher, C., Quail, M.A., Seshasayee, A.S., Luscombe, N.M., et al. ( 2008 ) The complete genome sequence of uropathogenic Proteus mirabilis, a master of both adherence and motility. J Bacteriol 190: 4027 – 4037. | en_US |
dc.identifier.citedreference | Rather, P.N. ( 2005 ) Swarmer cell differentiation in Proteus mirabilis. Environ Microbiol 7: 1065 – 1073. | en_US |
dc.identifier.citedreference | Simm, R., Morr, M., Kader, A., Nimtz, M., and RÖmling, U. ( 2004 ) GGDEF and EAL domains inversely regulate cyclic di-GMP levels and transition from sessility to motility. Mol Microbiol 53: 1123 – 1134. | en_US |
dc.identifier.citedreference | Tolson, D.L., Barrigar, D.L., McLean, R.J., and Altman, E. ( 1995 ) Expression of a nonagglutinating fimbria by Proteus mirabilis. Infect Immun 63: 1127 – 1129. | en_US |
dc.identifier.citedreference | Wray, S.K., Hull, S.I., Cook, R.G., Barrish, J., and Hull, R.A. ( 1986 ) Identification and characterization of a uroepithelial cell adhesin from a uropathogenic isolate of Proteus mirabilis. Infect Immun 54: 43 – 49. | en_US |
dc.identifier.citedreference | Zhao, H., Li, X., Johnson, D.E., Blomfield, I., and Mobley, H.L. ( 1997 ) In vivo phase variation of MR/P fimbrial gene expression in Proteus mirabilis infecting the urinary tract. Mol Microbiol 23: 1009 – 1019. | en_US |
dc.owningcollname | Interdisciplinary and Peer-Reviewed |
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