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Transcriptional control of toxT , a regulatory gene in the ToxR regulon of Vibrio cholerae
dc.contributor.authorHiggins, Dairen E.en_US
dc.contributor.authorDiRita, Victor J.en_US
dc.date.accessioned2010-06-01T18:20:49Z
dc.date.available2010-06-01T18:20:49Z
dc.date.issued1994-10en_US
dc.identifier.citationHiggins, Dairen E.; DiRita, Victor J. (1994). "Transcriptional control of toxT , a regulatory gene in the ToxR regulon of Vibrio cholerae." Molecular Microbiology 14(1): 17-29. <http://hdl.handle.net/2027.42/71556>en_US
dc.identifier.issn0950-382Xen_US
dc.identifier.issn1365-2958en_US
dc.identifier.urihttps://hdl.handle.net/2027.42/71556
dc.identifier.urihttp://www.ncbi.nlm.nih.gov/sites/entrez?cmd=retrieve&db=pubmed&list_uids=7830555&dopt=citationen_US
dc.description.abstractCo-ordinate expression of many virulence genes in Vibrio cholerae is under the control of the ToxR and ToxT proteins. These proteins function in a regulatory cascade in which ToxR is required to activate toxT , and ToxT activates virulence genes. The precise mechanism for ToxR activation of toxT is unknown, but data presented in this report suggest a direct involvement of ToxR. Primer extension and gene fusion analyses identified a ToxR-regulated promoter directly upstream of toxT, immediately following a region of inverted repeats capable of terminating transcription. Gel mobility shift studies indicate that ToxR binds DNA within the inverted repeat region, yet preliminary evidence suggests that ToxR binding alone is not sufficient for activation of toxT. Possible mechanisms of ToxR-dependent toxT expression are discussed.en_US
dc.format.extent5261174 bytes
dc.format.extent3109 bytes
dc.format.mimetypeapplication/pdf
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dc.publisherBlackwell Publishing Ltden_US
dc.rights1994 Blackwell Publishingen_US
dc.titleTranscriptional control of toxT , a regulatory gene in the ToxR regulon of Vibrio choleraeen_US
dc.typeArticleen_US
dc.subject.hlbsecondlevelMicrobiology and Immunologyen_US
dc.subject.hlbtoplevelScienceen_US
dc.description.peerreviewedPeer Revieweden_US
dc.contributor.affiliationumDepartment of Microbiology and Immunology, University of Michigan Medical School Ann Arbor, Michigan 48109, USA.en_US
dc.contributor.affiliationumUnit for Laboratory Animal Medicine, University of Michigan Medical School Ann Arbor, Michigan 48109, USA.en_US
dc.identifier.pmid7830555en_US
dc.description.bitstreamurlhttp://deepblue.lib.umich.edu/bitstream/2027.42/71556/1/j.1365-2958.1994.tb01263.x.pdf
dc.identifier.doi10.1111/j.1365-2958.1994.tb01263.xen_US
dc.identifier.sourceMolecular Microbiologyen_US
dc.identifier.citedreferenceAdhya, S., and Miller, W. ( 1979 ) Modulation of the two promoters of the galactose operon of Escherichia coli. Nature 279: 492 – 494.en_US
dc.identifier.citedreferenceAkerley, B.J., and Miller, J.F. ( 1993 ) Flagellin gene transcription In Bordetella bronchiseptica is egulated by the BvgAS virulence control system. J Bacteriol 175: 3468 – 3479.en_US
dc.identifier.citedreferenceBelasco, J.G., and Higgins, C.F. ( 1988 ) Mechanisms of mRNA decay in bacteria: a perspective. Gene 72: 15 – 23.en_US
dc.identifier.citedreferenceBtomfield, I.C., McClain, M.S., and Eisenstein, B.I. ( 1991 ) Type 1 fimbriae mutants of Escherichia coli K12: characterization of recognized afimbriate strains and construction of new fim deletion mutants. Mol Microbiol 5: 1439 – 1445.en_US
dc.identifier.citedreferenceBoorstein, W.R., and Craig, E.A. ( 1989 ) Primer extension analysis of RNA. Meth Enzymol 180: 347 – 369.en_US
dc.identifier.citedreferenceCamilli, A., Tilney, L.G., and Portnoy, D.A. ( 1993 ) Dual roles of plcA in Listeria monocytogenes pathogenesis. Mol Microbiol 8: 143 – 157.en_US
dc.identifier.citedreferenceChang, A.C.Y., and Cohen, S.N. ( 1978 ) Construction and characterization of amplifiable multicopy DNA cloning vehicles derived from the P15A cryptic miniplasmid. J Bacteriol 134: 1141 – 1156.en_US
dc.identifier.citedreferenceCheng, S.-W.C., Lynch, E.C., Leason, K.R., Court, D.L., Shapiro, B.A., and Friedman, D.I. ( 1991 ) Functional importance of sequence in the stem-loop of a transcription terminator. Science 254: 1205 – 1207.en_US
dc.identifier.citedreferenceDiRita, V.J. ( 1992 ) Co-ordinate expression of virulence genes by ToxR in Vibrio cholerae. Mol Microbiol 6: 451 – 458.en_US
dc.identifier.citedreferenceDiRita, V.J., and Mekalanos, J.J. ( 1991 ) Periplasmic interaction between two membrane regulatory proteins, ToxR and ToxS, results in signal transduction and transcriptional activation. Cell 64: 29 – 37.en_US
dc.identifier.citedreferenceDiRita, V.J., Parsot, C., Jander, G., and Mekalanos, J.J. ( 1991 ) Regulatory cascade controls virulence in Vibrio cholerae. Proc Natl Acad Sci USA 88: 5403 – 5407.en_US
dc.identifier.citedreferenceDorman, C.J., Bhriain, N.N., and Higgins, C.F. ( 1990 ) DNA supercoiling and environmental regulation of virulence gene expression in Shigella flexneri. Nature 344: 789 – 792.en_US
dc.identifier.citedreferenceElliott, T. ( 1992 ) A method for constructing single-copy lac fusions in Salmonella typhimurium and its application to the hemA-prfA operon. J Bacteriol 174: 245 – 253.en_US
dc.identifier.citedreferenceGalan, J.E., and Curtiss III, R. ( 1990 ) Expression of Salmonella typhimurium genes required for invasion is regulated by changes in DNA supercoiling. Infect Immun 58: 1879 – 1885.en_US
dc.identifier.citedreferenceHerrington, D.A., Hall, R.H., Losonsky, G., Mekalanos, J.J., Taylor, R.K., and Levine, M.M. ( 1988 ) Toxin, toxin-coregulated pili, and the ToxR regulon are essential for Vibrio cholerae pathogenesis in humans. J Exp Med 168: 1487 – 1492.en_US
dc.identifier.citedreferenceHiggins, D.E., Nazareno, E., and DiRita, V.J. ( 1992 ) The virulence gene activator ToxT from Vibrio cholerae is a member of the AraC family of transcriptional activators. J Bacteriol 174: 6974 – 6980.en_US
dc.identifier.citedreferenceHulton, C.S.J., Seirafi, A., Hinton, J.C.D., Sidebotham, J.M., Waddell, L., Pavitt, G.D., Owen-Hughes, T., Spassky, A., Buc, H., and Higgins, C.F. ( 1990 ) Histone-like protein H1(H-NS), DNA supercoiling and gene expression in bacteria. Gene 63: 631 – 642.en_US
dc.identifier.citedreferenceKaufman, M.R., Seyer, J.M., and Taylor, R.K. ( 1991 ) Processing of TCP pilin by TcpJ typifies a common step intrinsic to a newly recognized pathway of extracellular protein secretion by gram-negative bacteria. Genes Dev 5: 1834 – 1846.en_US
dc.identifier.citedreferenceKenney, T.J., and Moran, C.P., Jr. ( 1987 ) Organization and regulation of an operon that encodes a sporulation-essential sigma factor in Bacillus subtilis. J Bacteriol 169: 3329 – 3339.en_US
dc.identifier.citedreferenceLinn, T., and Pierre, R.S. ( 1990 ) Improved vector system for constructing transcriptional fusions that ensure independent translation of lacZ. J Bacteriol 172: 1077 – 1084.en_US
dc.identifier.citedreferenceMekalanos, J.J., Swartz, D.J., Pearson, G.D.N., Harford, N., Groyne, F., and de Wilde, M. ( 1983 ) Cholera toxin genes: nucleotide sequence, deletion analysis and vaccine development. Nature 306: 551 – 557.en_US
dc.identifier.citedreferenceMiller, J.H. ( 1972 ) Experiments in Molecular Genetics. Cold Spring Harbor, New York: Cold Spring Harbor Laboratory Press.en_US
dc.identifier.citedreferenceMiller, V.L., and Mekalanos, J.J. ( 1984 ) Synthesis of cholera toxin is positively regulated at the transcriptional level by ToxR. Proc Natl Acad Sci USA 81: 3471 – 3475.en_US
dc.identifier.citedreferenceMiller, V.L., DiRita, V.J., and Mekalanos, J.J. ( 1989 ) Identification of toxS, a regulatory gene whose product enhances ToxR-mediated activation of the cholera toxin promoter. J Bacteriol 171: 1288 – 1293.en_US
dc.identifier.citedreferenceMiller, V.L., Taylor, R.K., and Mekalanos, J.J. ( 1987 ) Cholera toxin transcriptional activator ToxR is a transmembrane DNA binding protein. Cell 48: 271 – 279.en_US
dc.identifier.citedreferenceMorales, V.M., Backman, A., and Bagdasarian, M. ( 1991 ) A series of wide-host-range low-copy-number vectors that allow direct screening for recombinants. Gene 97: 39 – 47.en_US
dc.identifier.citedreferenceOgierman, M.A., and Manning, P.A. ( 1992 ) Homology of TcpN, a putative regulatory protein of Vibrio cholerae, to the AraC family of transcriptional activators. Gene 116: 93 – 97.en_US
dc.identifier.citedreferenceOgierman, M.A., Zabihi, S., Mourtzios, L., and Manning, P.A. ( 1993 ) Genetic organization and sequence of the promoter-distal region of the tcp gene cluster of Vibrio cholerae. Gene 126: 51 – 60.en_US
dc.identifier.citedreferenceOttemann, K.M., DiRita, V.J., and Mekalanos, J.J. ( 1992 ) ToxR proteins with substitutions in residues conserved with OmpR fail to activate transcription from the cholera toxin promoter. J Bacteriol 174: 6807 – 6814.en_US
dc.identifier.citedreferenceParsot, C., and Mekalanos, J.J. ( 1992 ) Structural analysis of the acfA and acfD genes of Vibrio cholerae: effects of DNA topology and transcriptional activators on expression. J Bacteriol 174: 5211 – 5218.en_US
dc.identifier.citedreferencePeterson, K.M., and Mekalanos, J.J. ( 1988 ) Characterization of the Vibrio cholerae ToxR regulon: identification of novel genes involved in intestinal colonization. ( Published erratum appears in Infect immun ( 1989 ) 57: 660.) infect Immun 56: 2822 – 2829.en_US
dc.identifier.citedreferenceRaibaud, O., and Schwartz, M. ( 1984 ) Positive control of transcription initiation in bacteria. Annu Rev Genet 18: 173 – 206.en_US
dc.identifier.citedreferenceSambrook, J., Fritsch, E.F., and Maniatis, T. ( 1989 ) Molecular Cloning: A Laboratory Manual. 2nd edn. Cold Spring Harbor, New York: Cold Spring Harbor Laboratory Press.en_US
dc.identifier.citedreferenceSimons, R.W., Houman, F., and Kleckner, N. ( 1987 ) Improved single and multicopy lac -based cloning vectors for protein and operon fusions. Gene 53: 85 – 96.en_US
dc.identifier.citedreferenceStock, J.B., Ninfa, A., and Stock, A.M. ( 1989 ) Protein phosphorylation and regulation of adaptive responses in bacteria. Microbiol Rev 53: 450 – 490.en_US
dc.identifier.citedreferenceStraus, D.B., Walter, W.A., and Gross, C.A. ( 1987 ) The heat shock response of E. coli is regulated by changes in the concentration of sigma 32. Nature 329: 348 – 351.en_US
dc.identifier.citedreferenceTaylor, R.K., Miller, V.L, Furlong, D.B., and Mekalanos, J.J. ( 1987 ) Use of phoA gene fusions to identify a pilus colonization factor coordinately regulated with cholera toxin. Proc Natl Acad Sci USA 84: 2833 – 2837.en_US
dc.owningcollnameInterdisciplinary and Peer-Reviewed


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