Haem acquisition is facilitated by a novel receptor Hma and required by uropathogenic Escherichia coli for kidney infection
dc.contributor.author | Hagan, Erin Courtney | en_US |
dc.contributor.author | Mobley, Harry L. T. | en_US |
dc.date.accessioned | 2010-06-01T18:22:54Z | |
dc.date.available | 2010-06-01T18:22:54Z | |
dc.date.issued | 2009-01 | en_US |
dc.identifier.citation | Hagan, Erin C.; Mobley, Harry L. T. (2009). "Haem acquisition is facilitated by a novel receptor Hma and required by uropathogenic Escherichia coli for kidney infection." Molecular Microbiology 71(1): 79-91. <http://hdl.handle.net/2027.42/71590> | en_US |
dc.identifier.issn | 0950-382X | en_US |
dc.identifier.issn | 1365-2958 | en_US |
dc.identifier.uri | https://hdl.handle.net/2027.42/71590 | |
dc.identifier.uri | http://www.ncbi.nlm.nih.gov/sites/entrez?cmd=retrieve&db=pubmed&list_uids=19019144&dopt=citation | en_US |
dc.description.abstract | Iron acquisition, mediated by specific outer membrane receptors, is critical for colonization of the urinary tract by uropathogenic Escherichia coli (UPEC). The role of specific iron sources in vivo , however, remains largely unknown. In this study, we identified a 79 kDa haem receptor, h ae m a cquisition protein Hma, and established that it functions independently of ChuA to mediate haemin uptake by UPEC strain CFT073. We demonstrated that expression of hma promotes TonB-dependent haemin utilization and the Hma protein binds haemin with high affinity ( K d = 8 μM). Hma, however, lacks conserved His residues shown to mediate haem uptake by other bacterial receptors. In contrast, we identified Tyr-126 as a residue necessary for Hma-mediated haemin utilization. In a murine co-infection model of UTI, an isogenic hma mutant was out-competed by wild-type CFT073 in the kidneys ( P < 0.001) and spleens ( P < 0.0001) of infected mice, indicating its expression provided a competitive advantage in these organs. Furthermore, a hma chuA double mutant, which is unable to utilize haemin, was unable to colonize the kidneys to wild-type levels during independent infection ( P = 0.02). Thus, we demonstrate that UPEC requires haem for kidney colonization and that uptake of this iron source is mediated, in part, by the novel receptor, Hma. | en_US |
dc.format.extent | 366519 bytes | |
dc.format.extent | 3109 bytes | |
dc.format.mimetype | application/pdf | |
dc.format.mimetype | text/plain | |
dc.publisher | Blackwell Publishing Ltd | en_US |
dc.rights | Journal compilation © 2009 Blackwell Publishing | en_US |
dc.title | Haem acquisition is facilitated by a novel receptor Hma and required by uropathogenic Escherichia coli for kidney infection | en_US |
dc.type | Article | en_US |
dc.subject.hlbsecondlevel | Microbiology and Immunology | en_US |
dc.subject.hlbtoplevel | Science | en_US |
dc.description.peerreviewed | Peer Reviewed | en_US |
dc.identifier.pmid | 19019144 | en_US |
dc.description.bitstreamurl | http://deepblue.lib.umich.edu/bitstream/2027.42/71590/1/j.1365-2958.2008.06509.x.pdf | |
dc.identifier.doi | 10.1111/j.1365-2958.2008.06509.x | en_US |
dc.identifier.source | Molecular Microbiology | en_US |
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dc.owningcollname | Interdisciplinary and Peer-Reviewed |
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