Molecular cloning and characterization of a proline iminopeptidase gene from Neisseria gonorrhoeae
dc.contributor.author | Albertson, Nan H. | en_US |
dc.contributor.author | Koomey, Michael | en_US |
dc.date.accessioned | 2010-06-01T18:28:23Z | |
dc.date.available | 2010-06-01T18:28:23Z | |
dc.date.issued | 1993-09 | en_US |
dc.identifier.citation | Albertson, N. H.; Koomey, M. (1993). "Molecular cloning and characterization of a proline iminopeptidase gene from Neisseria gonorrhoeae ." Molecular Microbiology 9(6): 1203-1211. <http://hdl.handle.net/2027.42/71680> | en_US |
dc.identifier.issn | 0950-382X | en_US |
dc.identifier.issn | 1365-2958 | en_US |
dc.identifier.uri | https://hdl.handle.net/2027.42/71680 | |
dc.identifier.uri | http://www.ncbi.nlm.nih.gov/sites/entrez?cmd=retrieve&db=pubmed&list_uids=7934933&dopt=citation | en_US |
dc.description.abstract | Proline iminopeptidase (Pip) is a hydrolase elaborated by virtually all strains of Neisseria gonorrhoeae that selectively removes N -terminal proline residues from peptides. Escherichia coli clones expressing the gonococcal gene coding for Pip were identified in a genomic cosmid library using a synthetic colorimetric substrate. Nucleotide sequence determination and analyses of polypeptides detected by coupled in vitro transcription/translation reactions revealed that Pip is a 311-amino-acid polypeptide with a M r of 35 kDa and a pi of 5.4. Southern hybridization showed that the pip gene is present in a single copy on the chromosome of N. gonorrhoeae strain MS11 which maps immediately upstream of the previously identified opaA locus. The transcriptional start site of pip in E. coli , determined by primer extension analysis, was characteristic of an NtrA or sigma-54-dependent pro-motor. Complementation of an E. coli mutant deficient in both proline biosynthesis and dipeptide uptake confirmed that Pip is capable of releasing biologically active proline from peptides. Pip expression was found to be non-essential for in vitro growth of N. gonorrhoeae , based on the viability of a Pip − gonococcal mutant. | en_US |
dc.format.extent | 3465743 bytes | |
dc.format.extent | 3109 bytes | |
dc.format.mimetype | application/pdf | |
dc.format.mimetype | text/plain | |
dc.publisher | Blackwell Publishing Ltd | en_US |
dc.rights | 1993 Blackwell Publishing | en_US |
dc.title | Molecular cloning and characterization of a proline iminopeptidase gene from Neisseria gonorrhoeae | en_US |
dc.type | Article | en_US |
dc.subject.hlbsecondlevel | Microbiology and Immunology | en_US |
dc.subject.hlbtoplevel | Science | en_US |
dc.description.peerreviewed | Peer Reviewed | en_US |
dc.contributor.affiliationum | Department of Microbiology and Immunology, University of Michigan Medical School, 6643 Medical Science Building II, Ann Arbor, Michigan 48109-0620, USA. | en_US |
dc.identifier.pmid | 7934933 | en_US |
dc.description.bitstreamurl | http://deepblue.lib.umich.edu/bitstream/2027.42/71680/1/j.1365-2958.1993.tb01249.x.pdf | |
dc.identifier.doi | 10.1111/j.1365-2958.1993.tb01249.x | en_US |
dc.identifier.source | Molecular Microbiology | en_US |
dc.identifier.citedreference | Achtman, M., Mercer, A., Kusecek, B., Pohl, A., Heuzenroeder, M., Aaronson, W., Sutton, A., and Silver, R.P. ( 1983 ) Six widespread bacterial clones among Escherichia coli K1 isolates. Infect Immun 39: 315 – 335. | en_US |
dc.identifier.citedreference | Ausubel, F.M., Brent, R., Kingston, R.E., Moore, D.D., Seidman, J.G., Smith, J.A., and Struhl, K. ( 1987 ) Current Protocols in Molecular Biology. New York: John Wiley and Sons. | en_US |
dc.identifier.citedreference | BergstrÖm, S., Robbins, K., Koomey, J.M., and Swanson, J. ( 1986 ) Piliation control mechanisms in Neisseria gonorrhoeae. Proc Natl Acad Sci USA 83: 3890 – 3894. | en_US |
dc.identifier.citedreference | Bhat, K.S., Gibbs, C.P., Barrera, O., Morrison, S.G., Jahnig, F., Stern, A., Kupsch, E.M., Meyer, T.F., and Swanson, J. ( 1991 ) The opacity proteins of Neisseria gonorrhoeae strain MS11 are encoded by a family of 11 complete genes. Mol Microbiol 5: 1889 – 1901. ( erratum: Mol Microbiol 6: 1073–1076 ). | en_US |
dc.identifier.citedreference | Bihlmaier, A., RÖmling, U., Meyer, T.F., TÜmmler, B., and Gibbs, C.P. ( 1991 ) Physical and genetic map of the Neisseria gonorrhoeae strain MS11- N198 chromosome. Mol Microbiol 5: 2529 – 2539. | en_US |
dc.identifier.citedreference | Chen, K.C., and Buchanan, T.M. ( 1980 ) Hydrolases from Neisseria gonorrhoeae. The study of gonocosin, an aminopeptidase-P, a proline iminopeptidase, and an asparaginase. J Biol Chem 255: 1704 – 1710. | en_US |
dc.identifier.citedreference | Claverie-Martin, F., and Magasanik, B. ( 1991 ) Role of integration host factor in the regulation of the glnHp2 promotor of Escherichia coli. Proc Natl Acad Sci USA 88: 1631 – 1635. | en_US |
dc.identifier.citedreference | Close, T.J., and Rodriguez, R.L. ( 1982 ) Construction and characterization of the chloramphenicol-resistance gene cartridge: a new approach to the transcriptional mapping of extrachromosomal elements. Gene 20: 305 – 316. | en_US |
dc.identifier.citedreference | D'Amato, R.F., Enriquez, K.M., Tomfohrde, K.M., and Singer-man, E. ( 1978 ) Rapid identification of Neisseria gonorrhoeae and Neisseria meningitidis by using enzymatic profiles. J Clin Microbiol 7: 77 – 81. | en_US |
dc.identifier.citedreference | Goodman, S.D., and Scocca, J.J. ( 1988 ) Identification and arrangement of the DNA sequence recognized in specific transformation of Neisseria gonorrhoeae. Proc Natl Acad Sci USA 85: 6982 – 6986. | en_US |
dc.identifier.citedreference | Hessing, J.G.M. ( 1983 ) The primary structure of atropinesterase from Pseudomonas putida. Ph.D. Thesis. The Netherlands: University of Leiden. | en_US |
dc.identifier.citedreference | Horn, J.M., Harayama, S., and Timmis, K.N. ( 1991 ) DNA sequence determination of the TOL plasmid (pWWO) xylGFJ genes of Pseudomonas putida: implications for the evolution of aromatic catabolism. Mol Microbiol 5: 2459 – 2474. | en_US |
dc.identifier.citedreference | Ishimoto, K.S., and Lory, S. ( 1989 ) Formation of pilin in Pseudomonas aeruginosa requires the alternative sigma factor (RpoN) of RNA polymerase. Proc Natl Acad Sci USA 86: 1954 – 1957. | en_US |
dc.identifier.citedreference | Kitazono, A., Yoshimoto, T., and Tsuru, D. ( 1992 ) Cloning, sequencing and high expression of the proline iminopeptidase gene from Bacillus coagulans. J Bacteriol 174: 7919 – 7925. | en_US |
dc.identifier.citedreference | Knapp, J.S., and Holmes, K.K. ( 1975 ) Disseminated gonococcal infections caused by Neisseria gonorrhoeae with unique nutritional requirements. J Inf Dis 132: 204 – 208. | en_US |
dc.identifier.citedreference | Koomey, M., BergstrÖm, S., Blake, M., and Swanson, J. ( 1991 ) Pilin expression and processing in pilus mutants of Neisseria gonorrhoeae: critical role of Gly -1 in assembly. Mol Microbiol 5: 279 – 287. | en_US |
dc.identifier.citedreference | Kustu, S., Santero, E., Keener, J., Popham, D., and Weiss, O. ( 1989 ) Expression of Σ; 54 ( ntrA )-dependent genes is probably united by a common mechanism. Microbiol Rev 53: 367 – 376. | en_US |
dc.identifier.citedreference | MÄkinen, K.K. ( 1969 ) The proline iminopeptidases of the human oral cavity. Acta Chem Scand 23: 1409 – 1438. | en_US |
dc.identifier.citedreference | Meyer, T.F., Billyard, E., Hass, R., Storzbach, S., and So, M. ( 1984 ) Pilus genes of Neisseria gonorrhoeae: chromosomal organization and DNA sequence. Proc Natl Acad Sci USA 81: 6110 – 6114. | en_US |
dc.identifier.citedreference | Miller, J.H. ( 1982 ). Experiments in Molecular Genetics. Cold Spring Harbor, New York: Cold Spring Harbor Laboratory Press. | en_US |
dc.identifier.citedreference | O'Farrell, P.H. ( 1975 ) High resolution two-dimensional electrophoresis of proteins. J Biol Chem 250: 4007 – 4021. | en_US |
dc.identifier.citedreference | Olson, E.R., Dunyak, D.S., Jurss, L.M., and Poorman, R.A. ( 1991 ) Identification and characterization of dppA, an Escherichia coli gene encoding a periplasmic dipeptide transport protein. J Bacteriol 173: 234 – 244. | en_US |
dc.identifier.citedreference | Sambrook, J., Fritsch, E.J., and Maniatis, T. ( 1989 ) Molecular Cloning: A Laboratory Manual. Cold Spring Harbor, New York: Cold Spring Harbor Laboratory Press. | en_US |
dc.identifier.citedreference | Sarid, S., Berger, A., and Katchalski, E. ( 1969 ) Proline iminopeptidase. J Biol Chem 234: 1740 – 1746. | en_US |
dc.identifier.citedreference | Sarid, S., Berger, A., and Katchalski, E. ( 1962 ) Proline Iminopeptidase: purification and comparison with iminodipeptidase (prolinase). J Biol Chem 237: 2207 – 2212. | en_US |
dc.identifier.citedreference | Sperry, J.F., Cohenford, M.A., Campognone, P., Lawton, W., and Chee, D.O. ( 1986 ) Increased detection of prolylaminopeptidase in Neisseria meningitidis by Identicult-Neisseria. J Clin Microbiol 24: 145 – 145. | en_US |
dc.identifier.citedreference | Su, Y.A., Sulavik, M.C., He, P., Makinen, K.K., Makinen, P.L., Fiedler, S., Wirth, R., and Clewell, D.B. ( 1991 ) Nucleotide sequence of the gelatinase gene ( gelE ) from Enterococcus faecalis subsp. liquefaciens. Infect Immun 59: 415 – 420. | en_US |
dc.identifier.citedreference | Swanson, J., BergstrÖm, S., Barrera, O., Robbins, K., and Corwin, D. ( 1985 ) Pilus − gonococcal variants. Evidence for multiple forms of piliation control. J Exp Med 162: 729 – 744. | en_US |
dc.identifier.citedreference | VanBogelen, R.A., Hutton, M.E., and Neidhardt, F.C. ( 1990 ) Gene-protein database of Escherichia coli K-12: edition 3. Electrophoresis 11: 1131 – 1166. | en_US |
dc.identifier.citedreference | Yager, T.D., and von Hippel, P.H. ( 1987 ) Transcript elongation and termination in Escherichia coli. In Escherichia coli and Salmonella typhimurium: Cellular and Molecular Biology. Vol. 2. Neidhardt, F.C., Ingraham, J.L., Low, L.B., Magasanik, B., Schaechter, M., and Umbarger, H.E. ( eds ). Washington, D.C.: American Society for Microbiology, pp. 1241 – 1275. | en_US |
dc.identifier.citedreference | Yajko, D.M., Chu, A., and Hadley, W.K. ( 1984 ) Rapid confirmatoty identification of Neisseria gonorrhoeae with lectins and chromogenic substrates. J Clin Microbiol 19: 380 – 362. | en_US |
dc.identifier.citedreference | Yoshimoto, T., and Tsuru, D. ( 1985 ) Proline iminopeptidase from Bacillus coagulans: Purification and enzymatic properties. J Biochem 97: 1477 – 1485. | en_US |
dc.identifier.citedreference | Yoshimoto, T., Saeki, S., and Tsuru, D. ( 1980 ) Proline iminopeptidase from Bacillus megaterium: purification and characterization. J Biochem 93: 469 – 477. | en_US |
dc.identifier.citedreference | Zhang, Q.Y., DeRyckere, D., Lauer, P., and Koomey, M. ( 1992 ) Gene conversion in Neisseria gonorrhoeae: evidence for its role in pilus antigenic variation. Proc Natl Acad Sci USA 89: 5366 – 5370. | en_US |
dc.owningcollname | Interdisciplinary and Peer-Reviewed |
Files in this item
Remediation of Harmful Language
The University of Michigan Library aims to describe library materials in a way that respects the people and communities who create, use, and are represented in our collections. Report harmful or offensive language in catalog records, finding aids, or elsewhere in our collections anonymously through our metadata feedback form. More information at Remediation of Harmful Language.
Accessibility
If you are unable to use this file in its current format, please select the Contact Us link and we can modify it to make it more accessible to you.