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Secretion of curli fibre subunits is mediated by the outer membrane-localized CsgG protein
dc.contributor.authorRobinson, Lloyd S.en_US
dc.contributor.authorAshman, Elisabeth M.en_US
dc.contributor.authorHultgren, Scott J.en_US
dc.contributor.authorChapman, Matthew R.en_US
dc.date.accessioned2010-06-01T18:29:29Z
dc.date.available2010-06-01T18:29:29Z
dc.date.issued2006-02en_US
dc.identifier.citationRobinson, Lloyd S.; Ashman, Elisabeth M.; Hultgren, Scott J.; Chapman, Matthew R. (2006). "Secretion of curli fibre subunits is mediated by the outer membrane-localized CsgG protein." Molecular Microbiology 59(3): 870-881. <http://hdl.handle.net/2027.42/71698>en_US
dc.identifier.issn0950-382Xen_US
dc.identifier.issn1365-2958en_US
dc.identifier.urihttps://hdl.handle.net/2027.42/71698
dc.identifier.urihttp://www.ncbi.nlm.nih.gov/sites/entrez?cmd=retrieve&db=pubmed&list_uids=16420357&dopt=citationen_US
dc.description.abstractProduced by many Enterobacteriaceae spp., curli are biologically important amyloid fibres that have been associated with biofilm formation, host cell adhesion and invasion, and immune system activation. CsgA is the major fibre subunit and CsgE, CsgF and CsgG are non-structural proteins involved in curli biogenesis. We have characterized the role of CsgG in curli subunit secretion across the outer membrane. Directed mutagenesis of CsgG confirmed that its activity is dependent on localization to the outer membrane. Rotary Shadow electron microscopy of purified CsgG suggested that this protein assembles into an oligomeric complex with an apparent central pore. Oligomeric CsgG complexes were confirmed using co-purification experiments. Antibiotic sensitivity assays demonstrated that overexpression of CsgG rendered Escherichia coli susceptible to the antibiotic erythromycin. A 22-amino-acid sequence at the N-terminus of CsgA was sufficient to direct heterologous proteins to the CsgG secretion apparatus. Finally, we determined that CsgG participates in an outer membrane complex with two other curli assembly proteins, CsgE and CsgF.en_US
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dc.publisherBlackwell Science Ltden_US
dc.rights© 2005 The Authors; Journal compilation © 2005 Blackwell Publishing Ltden_US
dc.titleSecretion of curli fibre subunits is mediated by the outer membrane-localized CsgG proteinen_US
dc.typeArticleen_US
dc.subject.hlbsecondlevelMicrobiology and Immunologyen_US
dc.subject.hlbtoplevelScienceen_US
dc.description.peerreviewedPeer Revieweden_US
dc.contributor.affiliationumDepartment of Molecular, Cellular and Developmental Biology, University of Michigan, 830 North University, Ann Arbor, MI 48109, USA.en_US
dc.contributor.affiliationotherDepartment of Molecular Microbiology and Microbial Pathogenesis, Box 8230, Washington University School of Medicine, 660S. Euclid Ave, St Louis, MO 63110, USA.en_US
dc.identifier.pmid16420357en_US
dc.description.bitstreamurlhttp://deepblue.lib.umich.edu/bitstream/2027.42/71698/1/j.1365-2958.2005.04997.x.pdf
dc.description.bitstreamurlhttp://deepblue.lib.umich.edu/bitstream/2027.42/71698/2/MMI4997TableS1.pdf
dc.identifier.doi10.1111/j.1365-2958.2005.04997.xen_US
dc.identifier.sourceMolecular Microbiologyen_US
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dc.owningcollnameInterdisciplinary and Peer-Reviewed


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