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The CprS sensor kinase of the zoonotic pathogen Campylobacter jejuni influences biofilm formation and is required for optimal chick colonization
dc.contributor.authorSvensson, Sarah L.en_US
dc.contributor.authorDavis, Lindsay M.en_US
dc.contributor.authorMacKichan, Joanna K.en_US
dc.contributor.authorAllan, Brenda J.en_US
dc.contributor.authorPajaniappan, Mohanasundarien_US
dc.contributor.authorThompson, Stuart A.en_US
dc.contributor.authorGaynor, Erin C.en_US
dc.date.accessioned2010-06-01T18:38:17Z
dc.date.available2010-06-01T18:38:17Z
dc.date.issued2009-01en_US
dc.identifier.citationSvensson, Sarah L.; Davis, Lindsay M.; MacKichan, Joanna K.; Allan, Brenda J.; Pajaniappan, Mohanasundari; Thompson, Stuart A.; Gaynor, Erin C. (2009). "The CprS sensor kinase of the zoonotic pathogen Campylobacter jejuni influences biofilm formation and is required for optimal chick colonization." Molecular Microbiology 71(1): 253-272. <http://hdl.handle.net/2027.42/71839>en_US
dc.identifier.issn0950-382Xen_US
dc.identifier.issn1365-2958en_US
dc.identifier.urihttps://hdl.handle.net/2027.42/71839
dc.identifier.urihttp://www.ncbi.nlm.nih.gov/sites/entrez?cmd=retrieve&db=pubmed&list_uids=19017270&dopt=citationen_US
dc.description.abstractCampylobacter jejuni , a prevalent cause of bacterial gastroenteritis, must adapt to different environments to be a successful pathogen. We previously identified a C. jejuni two-component regulatory system (Cj1226/7c) as upregulated during cell infections. Analyses described herein led us to designate the system CprRS ( C ampylobacter p lanktonic growth r egulation). While the response regulator was essential, a cprS sensor kinase mutant was viable. The δ cprS mutant displayed an apparent growth defect and formed dramatically enhanced and accelerated biofilms independent of upregulation of previously characterized surface polysaccharides. δ cprS also displayed a striking dose-dependent defect for colonization of chicks and was modestly enhanced for intracellular survival in INT407 cells. Proteomics analyses identified changes consistent with modulation of essential metabolic genes, upregulation of stress tolerance proteins, and increased expression of MOMP and FlaA. Consistent with expression profiling, we observed enhanced motility and secretion in δ cprS , and decreased osmotolerance and oxidative stress tolerance. We also found that C. jejuni biofilms contain a DNase I-sensitive component and that biofilm formation is influenced by deoxycholate and the metabolic substrate fumarate. These results suggest that CprRS influences expression of factors important for biofilm formation, colonization and stress tolerance, and also add to our understanding of C. jejuni biofilm physiology.en_US
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dc.publisherBlackwell Publishing Ltden_US
dc.rightsJournal compilation © 2009 Blackwell Publishingen_US
dc.titleThe CprS sensor kinase of the zoonotic pathogen Campylobacter jejuni influences biofilm formation and is required for optimal chick colonizationen_US
dc.typeArticleen_US
dc.subject.hlbsecondlevelMicrobiology and Immunologyen_US
dc.subject.hlbtoplevelScienceen_US
dc.description.peerreviewedPeer Revieweden_US
dc.contributor.affiliationumDepartment of Microbiology and Immunology, University of Michigan Medical School, Ann Arbor, MI, USA.en_US
dc.contributor.affiliationotherDepartment of Microbiology and Immunology, University of British Columbia, Vancouver, British Columbia, Canada.en_US
dc.contributor.affiliationotherInstitute of Environmental Science and Research, Porirua, New Zealand.en_US
dc.contributor.affiliationotherVaccine and Infectious Disease Organization, Saskatoon, Saskatchewan, Canada.en_US
dc.contributor.affiliationotherDepartment of Biochemistry and Molecular Biology, Medical College of Georgia, Augusta, GA, USA.en_US
dc.identifier.pmid19017270en_US
dc.description.bitstreamurlhttp://deepblue.lib.umich.edu/bitstream/2027.42/71839/1/MMI_6534_sm_Tables_S1-S3.pdf
dc.description.bitstreamurlhttp://deepblue.lib.umich.edu/bitstream/2027.42/71839/2/j.1365-2958.2008.06534.x.pdf
dc.identifier.doi10.1111/j.1365-2958.2008.06534.xen_US
dc.identifier.sourceMolecular Microbiologyen_US
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