Isolation and Chemical Composition of Dinoflagellate Nuclei *
dc.contributor.author | Rizzo, P. J. | en_US |
dc.contributor.author | Noodén, L. D. | en_US |
dc.date.accessioned | 2010-06-01T18:48:40Z | |
dc.date.available | 2010-06-01T18:48:40Z | |
dc.date.issued | 1973-11 | en_US |
dc.identifier.citation | RIZZO, P. J.; NOODÉN, L. D. (1973). "Isolation and Chemical Composition of Dinoflagellate Nuclei * ." Journal of Eukaryotic Microbiology 20(5): 666-672. <http://hdl.handle.net/2027.42/72006> | en_US |
dc.identifier.issn | 1066-5234 | en_US |
dc.identifier.issn | 1550-7408 | en_US |
dc.identifier.uri | https://hdl.handle.net/2027.42/72006 | |
dc.description.abstract | Nuclei were isolated from Peridinium cinctum. Peridinium trochoideum, Gyrodinium cohnii ( Cryptothecodinium cohnii ) and Gymnodinium nelsoni. The nuclei of G. cohnii, P. trochoideum and G. nelsoni were found to contain ∼ 6.9, 34 and 143 picograms of DNA respectively. The ratios to DNA of RNA, acid-soluble and acid-insoluble protein for G. cohnii were 0.32, 0.13 and 0.99, respectively. The corresponding values for P. trochoideum were 0.22, 0.08 and 1.22, while those for G. nelsoni were 0.21, 0.10 and 1.09. The chemical composition of dinoflagellate nuclei is compared and contrasted with that of typical eukaryotic nuclei. The culture of these difficult organisms also is discussed. | en_US |
dc.format.extent | 1574517 bytes | |
dc.format.extent | 3109 bytes | |
dc.format.mimetype | application/pdf | |
dc.format.mimetype | text/plain | |
dc.publisher | Blackwell Publishing Ltd | en_US |
dc.rights | 1973 by the Society of Protozoologists | en_US |
dc.subject.other | Peridinium Cinctum | en_US |
dc.subject.other | P. Trochoideum | en_US |
dc.subject.other | Gyrodinium Cohnii | en_US |
dc.subject.other | Gymnodinium Nelsoni | en_US |
dc.subject.other | Culture | en_US |
dc.subject.other | Nuclear Isolation | en_US |
dc.subject.other | Nuclear Composition | en_US |
dc.title | Isolation and Chemical Composition of Dinoflagellate Nuclei * | en_US |
dc.type | Article | en_US |
dc.subject.hlbsecondlevel | Biological Chemistry | en_US |
dc.subject.hlbtoplevel | Science | en_US |
dc.description.peerreviewed | Peer Reviewed | en_US |
dc.contributor.affiliationum | Department of Botany, University of Michigan, Ann Arbor, Michigan 48104 | en_US |
dc.description.bitstreamurl | http://deepblue.lib.umich.edu/bitstream/2027.42/72006/1/j.1550-7408.1973.tb03595.x.pdf | |
dc.identifier.doi | 10.1111/j.1550-7408.1973.tb03595.x | en_US |
dc.identifier.source | Journal of Eukaryotic Microbiology | en_US |
dc.identifier.citedreference | Bhargava, M. M. & Halvorson, H. O. 1971. Isolation of nuclei from yeast. J. Cell Biol. 49, 423 – 9. | en_US |
dc.identifier.citedreference | Britten, R. J. & Kohne, D. E. 1968. Repeated sequences in DNA. Science 161, 529 – 40. | en_US |
dc.identifier.citedreference | Burton, K. 1956. A study of the conditions and mechanism of the diphenylamine reaction for the colorimetric estimation of deoxyribonucleic acid. Biochem. J. 62, 315 – 22. | en_US |
dc.identifier.citedreference | Carefoot, J. R. 1968. Culture and heterotrophy of the freshwater dinoflagellate Peridinium cinctum FA. ovoplanum Lindeman. J. Phycol. 4, 129 – 31. | en_US |
dc.identifier.citedreference | Chunosoff, L. & Hirchfield, H. 1967. Nuclear structure and mitosis in the dinoflagellate. Gonyaulax monilata. J. Protozool. 14, 157 – 63. | en_US |
dc.identifier.citedreference | D'Alessio, G. & Trim, A. R. 1968. A method for the isolation of nuclei from leaves. J. Exp. Bot. 19, 831 – 9. | en_US |
dc.identifier.citedreference | de Deken-Grenson, M. & de Deken, R. H. 1959. Elimination of substances interfering with nucleic acid estimation. Biochim. Biophys. Acta 31, 195 – 207. | en_US |
dc.identifier.citedreference | Dische, Z. 1955. Color reactions of nucleic acids and their constituents based on reactions of their sugars, in Chargaff, E. & Davidson, J. N., eds., The Nucleic Acids. Academic Press, New York, 1, 285 – 305. | en_US |
dc.identifier.citedreference | Dodge, J. D. 1966. The Dinophyceae, in Godward, M. B. E., ed., The Chromosomes of the Algae. Edward Arnold, New York, 96 – 115. | en_US |
dc.identifier.citedreference | Duffus, J. M. 1969. The isolation of nuclei from the yeast. Schizosaccharomyces pombe. Biochim. Biophys. Acta 195, 230 – 3. | en_US |
dc.identifier.citedreference | Dwivedi, R. S., Dutta, S. K. & Bloch, D. P. 1969. Isolation and characterization of chromatin from. Neurospora crassa. J. Cell Biol. 43, 51 – 8. | en_US |
dc.identifier.citedreference | Franker, C. K. 1970. Some properties of DNA from zooxanthellae harbored by an anemone. Anthopleura elegantissima. J. Phycol. 6, 299 – 306. | en_US |
dc.identifier.citedreference | Guillard, R. R. L. & Ryther, J. H. 1962. Studies on marine planktonic diatoms I. Cyclotella nana Hustedt and Detonula confervaca (Cleve) Gran. Can. J. Microbiol. 8, 229 – 39. | en_US |
dc.identifier.citedreference | Hamilton, R. H., Kunsch, U. & Temperli, A. 1972. Simple rapid procedures for isolation of tobacco leaf nuclei. Anal. Biochem. 49, 48 – 57. | en_US |
dc.identifier.citedreference | Holliday, R. 1970. The organization of DNA in eukaryotic chromosomes. Symp. Soc. Gen. Microbiol. 20, 359 – 80. | en_US |
dc.identifier.citedreference | Honda, S. I., Hongladarom, T. & Laties, G. G. 1966. A new isolation medium for plant organelles. J. Exp. Bot. 17, 460 – 72. | en_US |
dc.identifier.citedreference | Hymer, W. C. & Kuff, E. L. 1964. Isolation of nuclei from mammalian tissues through the use of Triton X-100. J. Histochem. Cytochem. 12, 359 – 63. | en_US |
dc.identifier.citedreference | Jensen, W. A. 1962. Botanical Histochemistry. Freeman, San Francisco. | en_US |
dc.identifier.citedreference | Kubai, D. F. & Ris, H. 1970. Chromosome structure. Ann. Rev. Genetics 4, 263 – 94. | en_US |
dc.identifier.citedreference | Kuehl, L. 1964. Isolation of plant nuclei. Z. Naturforsch. 19b, 525 – 32. | en_US |
dc.identifier.citedreference | Leighton, T. J., Dill, B. C., Stock, J. J. & Phillips, C. 1971. Absence of histones from the chromosomal proteins of fungi. Proc. Nat. Acad. Sci. U.S. 68, 677 – 80. | en_US |
dc.identifier.citedreference | LØvtrup-Rein, H. & McEwen, B. S. 1966. Isolation and fractionation of rat brain nuclei. J. Cell Biol. 30, 405 – 15. | en_US |
dc.identifier.citedreference | Lowry, O. H., Rosenbrough, J. N., Farr, A. L. & Randall, R. J. 1951. Protein measurement with the Folin phenol reagent. J. Biol. Chem. 193, 265 – 75. | en_US |
dc.identifier.citedreference | Mendiola, L. R., Price, C. A. & Guillard, R. R. L. 1966. Isolation of nuclei from a marine dinoflagellate. Science 153, 1661 – 3. | en_US |
dc.identifier.citedreference | Mitchell, P. & Moyle, J. 1967. Proton-transport phosphorylation, in Slater, E. C., Kaniuga, Z. & Wojtezak, L., eds., Biochemistry of Mitochondria. Academic Press, New York, 66 – 81. | en_US |
dc.identifier.citedreference | Munro, H. N. & Fleck, A. 1966. The determination of nucleic acids, in Glick, D., Methods of Biochemical Analysis. Interscience, New York, 14, 113 – 76. | en_US |
dc.identifier.citedreference | Ogur, M. & Resen, G. 1950. The nucleic acids of plant tissues. I. The extraction and estimation of desoxypentose nucleic acid and pentose nucleic acid. Arch. Biochem. 25, 262 – 76. | en_US |
dc.identifier.citedreference | Parenti, F., Brawerman, G., Preston, J. F. & Eisenstadt, J. M. 1967. Isolation of nuclei from. Euglena gracilis. Biochim. Biophys. Acta 195, 234 – 43. | en_US |
dc.identifier.citedreference | Provasoli, L. & Gold, K. 1962. Nutrition of the American strain of. Gyrodinium cohnii. Arch. Mikrobiol. 42, 196 – 203. | en_US |
dc.identifier.citedreference | Rizzo, P. J. & NoodÉn, L. D. 1972. Chromosomal proteins in the dinoflagellate alga. Gyrodinium cohnii. Science 176, 796 – 7. | en_US |
dc.identifier.citedreference | Rozijn, Th. H. & Tonino, G. J. M. 1964. Studies on the yeast nucleus. I. The isolation of nuclei. Biochim. Biophys. Acta 91, 105 – 12. | en_US |
dc.identifier.citedreference | Sitz, T. O., Kent, A. B., Hopkins, H. A. & Schmidt, R. R. 1970. Equilibrium density-gradient procedure for selection of synchronous cells from asynchronous cultures. Science 168, 1231 – 2. | en_US |
dc.identifier.citedreference | Starr, R. C. 1964. The culture collection of algae at Indiana University. Am. J. Bot. 51, 1013 – 44. | en_US |
dc.identifier.citedreference | Stern, H. 1967. Isolation and purification of plant nucleic acids from whole tissues and from isolated nuclei, in Grossman, P. & Moldave, K., eds., Methods in Enzymology, Academic Press, New York 12B, 100 – 12. | en_US |
dc.identifier.citedreference | Tautvydas, K. J. 1971. Mass isolation of pea nuclei. Plant Physiol. 47, 499 – 503. | en_US |
dc.owningcollname | Interdisciplinary and Peer-Reviewed |
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