Show simple item record

Isolation and Chemical Composition of Dinoflagellate Nuclei *

dc.contributor.authorRizzo, P. J.en_US
dc.contributor.authorNoodén, L. D.en_US
dc.date.accessioned2010-06-01T18:48:40Z
dc.date.available2010-06-01T18:48:40Z
dc.date.issued1973-11en_US
dc.identifier.citationRIZZO, P. J.; NOODÉN, L. D. (1973). "Isolation and Chemical Composition of Dinoflagellate Nuclei * ." Journal of Eukaryotic Microbiology 20(5): 666-672. <http://hdl.handle.net/2027.42/72006>en_US
dc.identifier.issn1066-5234en_US
dc.identifier.issn1550-7408en_US
dc.identifier.urihttps://hdl.handle.net/2027.42/72006
dc.description.abstractNuclei were isolated from Peridinium cinctum. Peridinium trochoideum, Gyrodinium cohnii ( Cryptothecodinium cohnii ) and Gymnodinium nelsoni. The nuclei of G. cohnii, P. trochoideum and G. nelsoni were found to contain ∼ 6.9, 34 and 143 picograms of DNA respectively. The ratios to DNA of RNA, acid-soluble and acid-insoluble protein for G. cohnii were 0.32, 0.13 and 0.99, respectively. The corresponding values for P. trochoideum were 0.22, 0.08 and 1.22, while those for G. nelsoni were 0.21, 0.10 and 1.09. The chemical composition of dinoflagellate nuclei is compared and contrasted with that of typical eukaryotic nuclei. The culture of these difficult organisms also is discussed.en_US
dc.format.extent1574517 bytes
dc.format.extent3109 bytes
dc.format.mimetypeapplication/pdf
dc.format.mimetypetext/plain
dc.publisherBlackwell Publishing Ltden_US
dc.rights1973 by the Society of Protozoologistsen_US
dc.subject.otherPeridinium Cinctumen_US
dc.subject.otherP. Trochoideumen_US
dc.subject.otherGyrodinium Cohniien_US
dc.subject.otherGymnodinium Nelsonien_US
dc.subject.otherCultureen_US
dc.subject.otherNuclear Isolationen_US
dc.subject.otherNuclear Compositionen_US
dc.titleIsolation and Chemical Composition of Dinoflagellate Nuclei *en_US
dc.typeArticleen_US
dc.subject.hlbsecondlevelBiological Chemistryen_US
dc.subject.hlbtoplevelScienceen_US
dc.description.peerreviewedPeer Revieweden_US
dc.contributor.affiliationumDepartment of Botany, University of Michigan, Ann Arbor, Michigan 48104en_US
dc.description.bitstreamurlhttp://deepblue.lib.umich.edu/bitstream/2027.42/72006/1/j.1550-7408.1973.tb03595.x.pdf
dc.identifier.doi10.1111/j.1550-7408.1973.tb03595.xen_US
dc.identifier.sourceJournal of Eukaryotic Microbiologyen_US
dc.identifier.citedreferenceBhargava, M. M. & Halvorson, H. O. 1971. Isolation of nuclei from yeast. J. Cell Biol. 49, 423 – 9.en_US
dc.identifier.citedreferenceBritten, R. J. & Kohne, D. E. 1968. Repeated sequences in DNA. Science 161, 529 – 40.en_US
dc.identifier.citedreferenceBurton, K. 1956. A study of the conditions and mechanism of the diphenylamine reaction for the colorimetric estimation of deoxyribonucleic acid. Biochem. J. 62, 315 – 22.en_US
dc.identifier.citedreferenceCarefoot, J. R. 1968. Culture and heterotrophy of the freshwater dinoflagellate Peridinium cinctum FA. ovoplanum Lindeman. J. Phycol. 4, 129 – 31.en_US
dc.identifier.citedreferenceChunosoff, L. & Hirchfield, H. 1967. Nuclear structure and mitosis in the dinoflagellate. Gonyaulax monilata. J. Protozool. 14, 157 – 63.en_US
dc.identifier.citedreferenceD'Alessio, G. & Trim, A. R. 1968. A method for the isolation of nuclei from leaves. J. Exp. Bot. 19, 831 – 9.en_US
dc.identifier.citedreferencede Deken-Grenson, M. & de Deken, R. H. 1959. Elimination of substances interfering with nucleic acid estimation. Biochim. Biophys. Acta 31, 195 – 207.en_US
dc.identifier.citedreferenceDische, Z. 1955. Color reactions of nucleic acids and their constituents based on reactions of their sugars, in Chargaff, E. & Davidson, J. N., eds., The Nucleic Acids. Academic Press, New York, 1, 285 – 305.en_US
dc.identifier.citedreferenceDodge, J. D. 1966. The Dinophyceae, in Godward, M. B. E., ed., The Chromosomes of the Algae. Edward Arnold, New York, 96 – 115.en_US
dc.identifier.citedreferenceDuffus, J. M. 1969. The isolation of nuclei from the yeast. Schizosaccharomyces pombe. Biochim. Biophys. Acta 195, 230 – 3.en_US
dc.identifier.citedreferenceDwivedi, R. S., Dutta, S. K. & Bloch, D. P. 1969. Isolation and characterization of chromatin from. Neurospora crassa. J. Cell Biol. 43, 51 – 8.en_US
dc.identifier.citedreferenceFranker, C. K. 1970. Some properties of DNA from zooxanthellae harbored by an anemone. Anthopleura elegantissima. J. Phycol. 6, 299 – 306.en_US
dc.identifier.citedreferenceGuillard, R. R. L. & Ryther, J. H. 1962. Studies on marine planktonic diatoms I. Cyclotella nana Hustedt and Detonula confervaca (Cleve) Gran. Can. J. Microbiol. 8, 229 – 39.en_US
dc.identifier.citedreferenceHamilton, R. H., Kunsch, U. & Temperli, A. 1972. Simple rapid procedures for isolation of tobacco leaf nuclei. Anal. Biochem. 49, 48 – 57.en_US
dc.identifier.citedreferenceHolliday, R. 1970. The organization of DNA in eukaryotic chromosomes. Symp. Soc. Gen. Microbiol. 20, 359 – 80.en_US
dc.identifier.citedreferenceHonda, S. I., Hongladarom, T. & Laties, G. G. 1966. A new isolation medium for plant organelles. J. Exp. Bot. 17, 460 – 72.en_US
dc.identifier.citedreferenceHymer, W. C. & Kuff, E. L. 1964. Isolation of nuclei from mammalian tissues through the use of Triton X-100. J. Histochem. Cytochem. 12, 359 – 63.en_US
dc.identifier.citedreferenceJensen, W. A. 1962. Botanical Histochemistry. Freeman, San Francisco.en_US
dc.identifier.citedreferenceKubai, D. F. & Ris, H. 1970. Chromosome structure. Ann. Rev. Genetics 4, 263 – 94.en_US
dc.identifier.citedreferenceKuehl, L. 1964. Isolation of plant nuclei. Z. Naturforsch. 19b, 525 – 32.en_US
dc.identifier.citedreferenceLeighton, T. J., Dill, B. C., Stock, J. J. & Phillips, C. 1971. Absence of histones from the chromosomal proteins of fungi. Proc. Nat. Acad. Sci. U.S. 68, 677 – 80.en_US
dc.identifier.citedreferenceLØvtrup-Rein, H. & McEwen, B. S. 1966. Isolation and fractionation of rat brain nuclei. J. Cell Biol. 30, 405 – 15.en_US
dc.identifier.citedreferenceLowry, O. H., Rosenbrough, J. N., Farr, A. L. & Randall, R. J. 1951. Protein measurement with the Folin phenol reagent. J. Biol. Chem. 193, 265 – 75.en_US
dc.identifier.citedreferenceMendiola, L. R., Price, C. A. & Guillard, R. R. L. 1966. Isolation of nuclei from a marine dinoflagellate. Science 153, 1661 – 3.en_US
dc.identifier.citedreferenceMitchell, P. & Moyle, J. 1967. Proton-transport phosphorylation, in Slater, E. C., Kaniuga, Z. & Wojtezak, L., eds., Biochemistry of Mitochondria. Academic Press, New York, 66 – 81.en_US
dc.identifier.citedreferenceMunro, H. N. & Fleck, A. 1966. The determination of nucleic acids, in Glick, D., Methods of Biochemical Analysis. Interscience, New York, 14, 113 – 76.en_US
dc.identifier.citedreferenceOgur, M. & Resen, G. 1950. The nucleic acids of plant tissues. I. The extraction and estimation of desoxypentose nucleic acid and pentose nucleic acid. Arch. Biochem. 25, 262 – 76.en_US
dc.identifier.citedreferenceParenti, F., Brawerman, G., Preston, J. F. & Eisenstadt, J. M. 1967. Isolation of nuclei from. Euglena gracilis. Biochim. Biophys. Acta 195, 234 – 43.en_US
dc.identifier.citedreferenceProvasoli, L. & Gold, K. 1962. Nutrition of the American strain of. Gyrodinium cohnii. Arch. Mikrobiol. 42, 196 – 203.en_US
dc.identifier.citedreferenceRizzo, P. J. & NoodÉn, L. D. 1972. Chromosomal proteins in the dinoflagellate alga. Gyrodinium cohnii. Science 176, 796 – 7.en_US
dc.identifier.citedreferenceRozijn, Th. H. & Tonino, G. J. M. 1964. Studies on the yeast nucleus. I. The isolation of nuclei. Biochim. Biophys. Acta 91, 105 – 12.en_US
dc.identifier.citedreferenceSitz, T. O., Kent, A. B., Hopkins, H. A. & Schmidt, R. R. 1970. Equilibrium density-gradient procedure for selection of synchronous cells from asynchronous cultures. Science 168, 1231 – 2.en_US
dc.identifier.citedreferenceStarr, R. C. 1964. The culture collection of algae at Indiana University. Am. J. Bot. 51, 1013 – 44.en_US
dc.identifier.citedreferenceStern, H. 1967. Isolation and purification of plant nucleic acids from whole tissues and from isolated nuclei, in Grossman, P. & Moldave, K., eds., Methods in Enzymology, Academic Press, New York 12B, 100 – 12.en_US
dc.identifier.citedreferenceTautvydas, K. J. 1971. Mass isolation of pea nuclei. Plant Physiol. 47, 499 – 503.en_US
dc.owningcollnameInterdisciplinary and Peer-Reviewed


Files in this item

Show simple item record

Remediation of Harmful Language

The University of Michigan Library aims to describe library materials in a way that respects the people and communities who create, use, and are represented in our collections. Report harmful or offensive language in catalog records, finding aids, or elsewhere in our collections anonymously through our metadata feedback form. More information at Remediation of Harmful Language.

Accessibility

If you are unable to use this file in its current format, please select the Contact Us link and we can modify it to make it more accessible to you.