Bacillus anthracis requires siderophore biosynthesis for growth in macrophages and mouse virulence
dc.contributor.author | Cendrowski, Stephen | en_US |
dc.contributor.author | MacArthur, William | en_US |
dc.contributor.author | Hanna, Philip C. | en_US |
dc.date.accessioned | 2010-06-01T18:50:21Z | |
dc.date.available | 2010-06-01T18:50:21Z | |
dc.date.issued | 2004-01 | en_US |
dc.identifier.citation | Cendrowski, Stephen; MacArthur, William; Hanna, Philip (2004). " Bacillus anthracis requires siderophore biosynthesis for growth in macrophages and mouse virulence." Molecular Microbiology 51(2): 407-417. <http://hdl.handle.net/2027.42/72033> | en_US |
dc.identifier.issn | 0950-382X | en_US |
dc.identifier.issn | 1365-2958 | en_US |
dc.identifier.uri | https://hdl.handle.net/2027.42/72033 | |
dc.identifier.uri | http://www.ncbi.nlm.nih.gov/sites/entrez?cmd=retrieve&db=pubmed&list_uids=14756782&dopt=citation | en_US |
dc.description.abstract | Systemic anthrax infections can be characterized as proceeding in stages, beginning with an early intracellular establishment stage within phagocytes that is followed by extracelluar stages involving massive bacteraemia, sepsis and death. Because most bacteria require iron, and the host limits iron availability through homeostatic mechanisms, we hypothesized that B. anthracis requires a high-affinity mechanism of iron acquisition during its growth stages. Two putative types of siderophore synthesis operons, named B acillus a nthracis c atechol, bac (anthrabactin), and a nthrax s iderophore b iosynthesis, asb (anthrachelin), were identified. Directed gene deletions in both anthrabactin and anthrachelin pathways were generated in a B. anthracis (Sterne) 34F2 background resulting in mutations in asbA and bacCEBF . A decrease in siderophore production was observed during iron-depleted growth in both the δ asbA and δ bacCEBF strains, but only the δ asbA strain was attenuated for growth under these conditions. In addition, the δ asbA strain was severely attenuated both for growth in macrophages (Mφ) and for virulence in mice. In contrast, the δ bacCEBF strain did not differ phenotypically from the parental strain. These findings support a requirement for anthrachelin but not anthrabactin in iron assimilation during the intracellular stage of anthrax. | en_US |
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dc.format.extent | 3109 bytes | |
dc.format.mimetype | application/pdf | |
dc.format.mimetype | text/plain | |
dc.publisher | Blackwell Science Ltd | en_US |
dc.rights | 2003 Blackwell Publishing Ltd | en_US |
dc.title | Bacillus anthracis requires siderophore biosynthesis for growth in macrophages and mouse virulence | en_US |
dc.type | Article | en_US |
dc.subject.hlbsecondlevel | Microbiology and Immunology | en_US |
dc.subject.hlbtoplevel | Science | en_US |
dc.description.peerreviewed | Peer Reviewed | en_US |
dc.contributor.affiliationum | Department of Microbiology and Immunology, University of Michigan Medical School, Ann Arbor, Michigan, USA. | en_US |
dc.contributor.affiliationum | GeneWorks, Inc., Ann Arbor, Michigan, USA. | en_US |
dc.identifier.pmid | 14756782 | en_US |
dc.description.bitstreamurl | http://deepblue.lib.umich.edu/bitstream/2027.42/72033/1/j.1365-2958.2003.03861.x.pdf | |
dc.identifier.doi | 10.1046/j.1365-2958.2003.03861.x | en_US |
dc.identifier.source | Molecular Microbiology | en_US |
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dc.owningcollname | Interdisciplinary and Peer-Reviewed |
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