Characterization of a Bacillus anthracis spore coat-surface protein that influences coat-surface morphology
dc.contributor.author | Mallozzi, Michael | en_US |
dc.contributor.author | Bozue, Joel | en_US |
dc.contributor.author | Giorno, Rebecca | en_US |
dc.contributor.author | Moody, Krishna-Sulayman | en_US |
dc.contributor.author | Slack, Alex | en_US |
dc.contributor.author | Cote, Christopher | en_US |
dc.contributor.author | Qiu, Dengli | en_US |
dc.contributor.author | Wang, Rong | en_US |
dc.contributor.author | McKenney, Peter | en_US |
dc.contributor.author | Lai, Erh-Min | en_US |
dc.contributor.author | Maddock, Janine R. | en_US |
dc.contributor.author | Friedlander, Arthur | en_US |
dc.contributor.author | Welkos, Susan | en_US |
dc.contributor.author | Eichenberger, Patrick | en_US |
dc.contributor.author | Driks, Adam | en_US |
dc.date.accessioned | 2010-06-01T18:56:49Z | |
dc.date.available | 2010-06-01T18:56:49Z | |
dc.date.issued | 2008-12 | en_US |
dc.identifier.citation | Mallozzi, Michael; Bozue, Joel; Giorno, Rebecca; Moody, Krishna-Sulayman; Slack, Alex; Cote, Christopher; Qiu, Dengli; Wang, Rong; McKenney, Peter; Lai, Erh-Min; Maddock, Janine R.; Friedlander, Arthur; Welkos, Susan; Eichenberger, Patrick; Driks, Adam (2008). "Characterization of a Bacillus anthracis spore coat-surface protein that influences coat-surface morphology." FEMS Microbiology Letters 289(1): 110-117. <http://hdl.handle.net/2027.42/72138> | en_US |
dc.identifier.issn | 0378-1097 | en_US |
dc.identifier.issn | 1574-6968 | en_US |
dc.identifier.uri | https://hdl.handle.net/2027.42/72138 | |
dc.identifier.uri | http://www.ncbi.nlm.nih.gov/sites/entrez?cmd=retrieve&db=pubmed&list_uids=19054101&dopt=citation | en_US |
dc.description.abstract | Bacterial spores are encased in a multilayered proteinaceous shell, called the coat. In many Bacillus spp., the coat protects against environmental assault and facilitates germination. In Bacillus anthracis , the spore is the etiological agent of anthrax, and the functions of the coat likely contribute to virulence. Here, we characterize a B. anthracis spore protein, called CotΒ, which is encoded only in the genomes of the Bacillus cereus group. We found that CotΒ is synthesized specifically during sporulation and is assembled onto the spore coat surface. Our analysis of a cotΒ null mutant in the Sterne strain reveals that CotΒ has a role in determining coat-surface morphology but does not detectably affect germination. In the fully virulent Ames strain, a cotΒ null mutation has no effect on virulence in a murine model of B. anthracis infection. | en_US |
dc.format.extent | 473203 bytes | |
dc.format.extent | 3109 bytes | |
dc.format.mimetype | application/pdf | |
dc.format.mimetype | text/plain | |
dc.publisher | Blackwell Publishing Ltd | en_US |
dc.rights | © 2008 Federation of European Microbiological Societies. Published by Blackwell Publishing Ltd. All rights reserved | en_US |
dc.subject.other | Bacillus Anthracis | en_US |
dc.subject.other | Coat | en_US |
dc.subject.other | Germination | en_US |
dc.subject.other | Spore Assembly | en_US |
dc.subject.other | Anthrax | en_US |
dc.title | Characterization of a Bacillus anthracis spore coat-surface protein that influences coat-surface morphology | en_US |
dc.type | Article | en_US |
dc.subject.hlbsecondlevel | Microbiology and Immunology | en_US |
dc.subject.hlbtoplevel | Health Sciences | en_US |
dc.description.peerreviewed | Peer Reviewed | en_US |
dc.contributor.affiliationum | Department of Molecular, Cellular and Developmental Biology, University of Michigan, Ann Arbor, MI, USA; and | en_US |
dc.contributor.affiliationother | Department of Microbiology and Immunology, Loyola University Medical Center, Maywood, IL, USA ; | en_US |
dc.contributor.affiliationother | Bacteriology Division, United States Army Medical Research Institute of Infectious Diseases, Frederick, MD, USA ; | en_US |
dc.contributor.affiliationother | Department of Biological, Chemical, and Physical Sciences, Illinois Institute of Technology, Chicago, IL, USA ; | en_US |
dc.contributor.affiliationother | Department of Biology and Center for Genomics and Systems Biology, New York University, NY, USA ; | en_US |
dc.contributor.affiliationother | Institute of Plant and Microbial Biology, Academia Sinica, Taipei, Taiwan, Republic of China ; | en_US |
dc.contributor.affiliationother | Headquarters, United States Army Medical Research Institute of Infectious Diseases, Frederick, MD, USA | en_US |
dc.identifier.pmid | 19054101 | en_US |
dc.description.bitstreamurl | http://deepblue.lib.umich.edu/bitstream/2027.42/72138/1/j.1574-6968.2008.01380.x.pdf | |
dc.identifier.doi | 10.1111/j.1574-6968.2008.01380.x | en_US |
dc.identifier.source | FEMS Microbiology Letters | en_US |
dc.identifier.citedreference | Aronson AI & Fitz-James P ( 1976 ) Structure and morphogenesis of the bacterial spore coat. Bacteriol Rev 40: 360 – 402. | en_US |
dc.identifier.citedreference | Asai K, Takamatsu H, Iwano M, Kodama T, Watabe K & Ogasawara N ( 2001 ) The Bacillus subtilis yabQ gene is essential for formation of the spore cortex. Microbiology 147: 919 – 927. | en_US |
dc.identifier.citedreference | Bauer T, Little S, Stover AG & Driks A ( 1999 ) Functional regions of the Bacillus subtilis spore coat morphogenetic protein CotE. J Bacteriol 181: 7043 – 7051. | en_US |
dc.identifier.citedreference | Bozue JA, Parthasarathy N, Phillips LR, Cote CK, Fellows PF, Mendelson I, Shafferman A & Friedlander AM ( 2005 ) Construction of a rhamnose mutation in Bacillus anthracis affects adherence to macrophages but not virulence in guinea pigs. Microb Pathogenesis 38: 1 – 12. | en_US |
dc.identifier.citedreference | Chada VG, Sanstad EA, Wang R & Driks A ( 2003 ) Morphogenesis of Bacillus spore surfaces. J Bacteriol 185: 6255 – 6261. | en_US |
dc.identifier.citedreference | Donovan W, Zheng LB, Sandman K & Losick R ( 1987 ) Genes encoding spore coat polypeptides from Bacillus subtilis. J Mol Biol 196: 1 – 10. | en_US |
dc.identifier.citedreference | Driks A ( 1999 ) Bacillus subtilis spore coat. Microbiol Mol Biol R 63: 1 – 20. | en_US |
dc.identifier.citedreference | Eichenberger P, Jensen ST, Conlon EM et al. ( 2003 ) The sigmaE regulon and the identification of additional sporulation genes in Bacillus subtilis. J Mol Biol 327: 945 – 972. | en_US |
dc.identifier.citedreference | Giorno R, Bozue J, Cote C et al. ( 2007 ) Morphogenesis of the Bacillus anthracis spore. J Bacteriol 189: 691 – 705. | en_US |
dc.identifier.citedreference | Harwood CR & Cutting S ( 1990 ) Molecular Biological Methods for Bacillus. John Wiley and Sons, Sussex, UK. | en_US |
dc.identifier.citedreference | Henriques AO & Moran CP Jr ( 2006 ) Structure, assembly, and function of the spore surface layers. Annu Rev Microbiol 61: 555 – 558. | en_US |
dc.identifier.citedreference | Henriques AO, Costa TV, Martins LO & Zilhao R ( 2004 ) The functional architecture and assembly of the coat. Bacterial Spore Formers: Probiotics and Emerging Applications ( Ricca E, Henriques AO & Cutting SM, eds), pp. 65 – 86. Horizon Biosciences, Norfolk. | en_US |
dc.identifier.citedreference | Hilbert DW & Piggot PJ ( 2004 ) Compartmentalization of gene expression during Bacillus subtilis spore formation. Microbiol Mol Biol R 68: 234 – 262. | en_US |
dc.identifier.citedreference | Holt SC & Leadbetter ER ( 1969 ) Comparative ultrastructure of selected aerobic spore-forming bacteria: a freeze-etching study. Bacteriol Rev 33: 346 – 378. | en_US |
dc.identifier.citedreference | Isticato R, Cangiano G, Tran HT, Ciabattini A, Medaglini D, Oggioni MR, De Felice M, Pozzi G & Ricca E ( 2001 ) Surface display of recombinant proteins on Bacillus subtilis spores. J Bacteriol 183: 6294 – 6301. | en_US |
dc.identifier.citedreference | Kim H, Hahn M, Grabowski P, McPherson DC, Otte MM, Wang R, Ferguson CC, Eichenberger P & Driks A ( 2006 ) The Bacillus subtilis spore coat protein interaction network. Mol Microbiol 59: 487 – 502. | en_US |
dc.identifier.citedreference | Kim HS, Sherman D, Johnson F & Aronson AI ( 2004 ) Characterization of a major Bacillus anthracis spore coat protein and its role in spore inactivation. J Bacteriol 186: 2413 – 2417. | en_US |
dc.identifier.citedreference | Klobutcher LA, Ragkousi K & Setlow P ( 2006 ) The Bacillus subtilis spore coat provides “eat resistance” during phagocytic predation by the protozoan Tetrahymena thermophila. Proc Natl Acad Sci USA 103: 165 – 170. | en_US |
dc.identifier.citedreference | Koehler TM, Dai Z & Kaufman-Yarbray M ( 1994 ) Regulation of the Bacillus anthracis protective antigen gene: CO 2 and a trans -acting element activate transcription from one of two promoters. J Bacteriol 176: 586 – 595. | en_US |
dc.identifier.citedreference | Kuwana R, Kasahara Y, Fujibayashi M, Takamatsu H, Ogasawara N & Watabe K ( 2002 ) Proteomics characterization of novel spore proteins of Bacillus subtilis. Microbiology 148: 3971 – 3982. | en_US |
dc.identifier.citedreference | Lai EM, Phadke ND, Kachman MT, Giorno R, Vazquez S, Vazquez JA, Maddock JR & Driks A ( 2003 ) Proteomic analysis of the spore coats of Bacillus subtilis and Bacillus anthracis. J Bacteriol 185: 1443 – 1454. | en_US |
dc.identifier.citedreference | Leighton TJ & Doi RH ( 1971 ) The stability of messenger ribonucleic acid during sporulation in Bacillus subtilis. J Biol Chem 246: 3189 – 3195. | en_US |
dc.identifier.citedreference | Little S & Driks A ( 2001 ) Functional analysis of the Bacillus subtilis morphogenetic spore coat protein CotE. Mol Microbiol 42: 1107 – 1120. | en_US |
dc.identifier.citedreference | Little SF & Knudson GB ( 1986 ) Comparative efficacy of Bacillus anthracis live spore vaccine and protective antigen vaccine against anthrax in the guinea pig. Infect Immun 52: 509 – 512. | en_US |
dc.identifier.citedreference | Losick R, Youngman P & Piggot PJ ( 1986 ) Genetics of endospore formation in Bacillus subtilis. Annu Rev Genet 20: 625 – 669. | en_US |
dc.identifier.citedreference | Lyons CR, Lovchik J, Hutt J, Lipscomb MF, Wang E, Heninger S, Berliba L & Garrison K ( 2004 ) Murine model of pulmonary anthrax: kinetics of dissemination, histopathology, and mouse strain susceptibility. Infect Immun 72: 4801 – 4809. | en_US |
dc.identifier.citedreference | McPherson DC, Kim H, Hahn M, Wang R, Grabowski P, Eichenberger P & Driks A ( 2005 ) Characterization of the Bacillus subtilis spore morphogenetic coat protein CotO. J Bacteriol 187: 8278 – 8290. | en_US |
dc.identifier.citedreference | Mendelson I, Tobery S, Scorpio A, Bozue J, Shafferman A & Friedlander AM ( 2004 ) The NheA component of the non-hemolytic enterotoxin of Bacillus cereus is produced by Bacillus anthracis but is not required for virulence. Microb Pathogenesis 37: 149 – 154. | en_US |
dc.identifier.citedreference | Ozin AJ, Samford CS, Henriques AO & Moran CP Jr ( 2001 ) SpoVID guides SafA to the spore coat in Bacillus subtilis. J Bacteriol 183: 3041 – 3049. | en_US |
dc.identifier.citedreference | Perez-Casal J, Caparon MG & Scott JR ( 1991 ) Mry, a trans -acting positive regulator of the M protein gene of Streptococcus pyogenes with similarity to the receptor proteins of two-component regulatory systems. J Bacteriol 173: 2617 – 2624. | en_US |
dc.identifier.citedreference | Plomp M, Leighton TJ, Wheeler KE & Malkin AJ ( 2005 ) The high-resolution architecture and structural dynamics of Bacillus spores. Biophys J 88: 603 – 608. | en_US |
dc.identifier.citedreference | Plomp M, Leighton TJ, Wheeler KE, Hill HD & Malkin AJ ( 2007 ) In vitro high-resolution structural dynamics of single germinating bacterial spores. Proc Natl Acad Sci USA 104: 9644 – 9649. | en_US |
dc.identifier.citedreference | Pogliano J, Osborne N, Sharp MD, Abanes-De Mello A, Perez A, Sun YL & Pogliano K ( 1999 ) A vital stain for studying membrane dynamics in bacteria: a novel mechanism controlling septation during Bacillus subtilis sporulation. Mol Microbiol 31: 1149 – 1159. | en_US |
dc.identifier.citedreference | Pogliano K, Harry E & Losick R ( 1995 ) Visualization of the subcellular location of sporulation proteins in Bacillus subtilis using immunofluorescence microscopy. Mol Microbiol 18: 459 – 470. | en_US |
dc.identifier.citedreference | Sambrook J, Fritsch EF & Maniatis T ( 1989 ) Molecular Cloning: A Laboratory Manual. Cold Spring Harbor Laboratory, Cold Spring Harbor, NY. | en_US |
dc.identifier.citedreference | Santo LY & Doi RH ( 1974 ) Ultrastructural analysis during germination and outgrowth of Bacillus subtilis spores. J Bacteriol 120: 475 – 481. | en_US |
dc.identifier.citedreference | Setlow P ( 2006 ) Spores of Bacillus subtilis: their resistance to and killing by radiation, heat and chemicals. J Appl Microbiol 101: 514 – 525. | en_US |
dc.identifier.citedreference | van Ooij C, Eichenberger P & Losick R ( 2004 ) Dynamic patterns of subcellular protein localization during spore coat morphogenesis in Bacillus subtilis. J Bacteriol 186: 4441 – 4448. | en_US |
dc.identifier.citedreference | Wang R, Krishnamurthy SN, Jeong J-S, Driks A, Mehta M & Gingras BA ( 2007 ) Finger-printing species and strains of Bacilli spores by distinctive coat surface morphology. Langmuir 23: 10230 – 10234. | en_US |
dc.identifier.citedreference | Warth AD, Ohye DF & Murrell WG ( 1963 ) The composition and structure of bacterial spores. J Cell Biol 16: 579 – 592. | en_US |
dc.identifier.citedreference | Young SB & Setlow P ( 2003 ) Mechanisms of killing of Bacillus subtilis spores by hypochlorite and chlorine dioxide. J Appl Microbiol 95: 54 – 67. | en_US |
dc.identifier.citedreference | Zheng LB & Losick R ( 1990 ) Cascade regulation of spore coat gene expression in Bacillus subtilis. J Mol Biol 212: 645 – 660. | en_US |
dc.owningcollname | Interdisciplinary and Peer-Reviewed |
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