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Increased expression of biodegradative threonine dehydratase of Escherichia coli by DNA gyrase inhibitors
dc.contributor.authorSumantran, Venil N.en_US
dc.contributor.authorTranguch, Anthony J.en_US
dc.contributor.authorDatta, Prasantaen_US
dc.date.accessioned2010-06-01T19:09:31Z
dc.date.available2010-06-01T19:09:31Z
dc.date.issued1989-11en_US
dc.identifier.citationSumantran, Venil N.; Tranguch, Anthony J.; Datta, Prasanta (1989). "Increased expression of biodegradative threonine dehydratase of Escherichia coli by DNA gyrase inhibitors." FEMS Microbiology Letters 65(1-2): 37-40. <http://hdl.handle.net/2027.42/72344>en_US
dc.identifier.issn0378-1097en_US
dc.identifier.issn1574-6968en_US
dc.identifier.urihttps://hdl.handle.net/2027.42/72344
dc.description.abstractThe synthesis of inducible biodegradative threonine dehydratase of Escherichia coli increased several-fold in the presence of the DNA gyrase inhibitors, nalidixic acid and coumermycin. Temperature-sensitive gyrB mutants expressed higher levels of dehydratase as compared to an isogenic gyrB + strain. Immunoblotting experiments showed increased synthesis of the dehydratase protein in the presence of gyrase inhibitors; addition of rifampicin and chloramphenicol to cells actively synthesizing enzyme preventing new enzyme production. Increased expression of dehydratase by gyrase inhibitors was accompanied by relaxation of supercoiled DNA.en_US
dc.format.extent346642 bytes
dc.format.extent3109 bytes
dc.format.mimetypeapplication/pdf
dc.format.mimetypetext/plain
dc.publisherBlackwell Publishing Ltden_US
dc.rights1989 Federation of European Microbiological Societiesen_US
dc.subject.otherGene Regulationen_US
dc.subject.otherGyrase Inhibitoren_US
dc.subject.otherDNA Supercoilingen_US
dc.subject.otherTdc Operonen_US
dc.subject.otherEscherichia Colien_US
dc.titleIncreased expression of biodegradative threonine dehydratase of Escherichia coli by DNA gyrase inhibitorsen_US
dc.typeArticleen_US
dc.subject.hlbsecondlevelMicrobiology and Immunologyen_US
dc.subject.hlbtoplevelHealth Sciencesen_US
dc.description.peerreviewedPeer Revieweden_US
dc.contributor.affiliationumDepartment of Biological Chemistry, The University of Michigan, Ann Arbor, MI 48109-0606, U.S.A.en_US
dc.description.bitstreamurlhttp://deepblue.lib.umich.edu/bitstream/2027.42/72344/1/j.1574-6968.1989.tb03593.x.pdf
dc.identifier.doi10.1111/j.1574-6968.1989.tb03593.xen_US
dc.identifier.sourceFEMS Microbiology Lettersen_US
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dc.owningcollnameInterdisciplinary and Peer-Reviewed


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