Diffusion of Alexa Fluor 488-Conjugated Dendrimers in Rat Aortic Tissue
dc.contributor.author | Cho, Brenda S. | en_US |
dc.contributor.author | Roelofs, Karen J. | en_US |
dc.contributor.author | Majoros, Istvan J. | en_US |
dc.contributor.author | Baker, James R. Jr. | en_US |
dc.contributor.author | Stanley, James C. | en_US |
dc.contributor.author | Henke, Peter K. | en_US |
dc.contributor.author | Upchurch, Gilbert R. | en_US |
dc.date.accessioned | 2010-06-01T19:18:14Z | |
dc.date.available | 2010-06-01T19:18:14Z | |
dc.date.issued | 2006-11 | en_US |
dc.identifier.citation | CHO, BRENDA S . ; ROELOFS, KAREN J . ; MAJOROS, ISTVAN J . ; BAKER, JAMES R . ; STANLEY, JAMES C . ; HENKE, PETER K . ; UPCHURCH, GILBERT R . (2006). "Diffusion of Alexa Fluor 488-Conjugated Dendrimers in Rat Aortic Tissue." Annals of the New York Academy of Sciences 1085(1 The Abdominal Aortic Aneurysm: Genetics, Pathophysiology, and Molecular Biology ): 294-305. <http://hdl.handle.net/2027.42/72448> | en_US |
dc.identifier.issn | 0077-8923 | en_US |
dc.identifier.issn | 1749-6632 | en_US |
dc.identifier.uri | https://hdl.handle.net/2027.42/72448 | |
dc.identifier.uri | http://www.ncbi.nlm.nih.gov/sites/entrez?cmd=retrieve&db=pubmed&list_uids=17182946&dopt=citation | en_US |
dc.description.abstract | In this study, the distribution of labeled dendrimers in native and aneurysmal rat aortic tissue was examined. Adult male rats underwent infrarenal aorta perfusion with generation 5 (G5) acetylated Alexa Fluor 488-conjugated dendrimers for varying lengths of time. In a second set of experiments, rats underwent aortic elastase perfusion followed by aortic dendrimer perfusion 7 days later. Aortic diameters were measured prior to and postelastase perfusion, and again on the day of harvest. Aortas were harvested 0, 12, or 24 h postperfusion, fixed, and mounted. Native aortas were harvested and viewed as negative controls. Aortic cross-sections were viewed and imaged using confocal microscopy. Dendrimers were quantified (counts high-powered field). Results were evaluated by repeated measures ANOVA and Student's t -test. We found that in native aortas, dendrimers penetrated the aortic wall in all groups. For all perfusion times, fewer dendrimers were present as time between dendrimer perfusion and aortic harvest increased. Longer perfusion times resulted in increased diffusion of dendrimers throughout the aortic wall. By 24 h, the majority of the dendrimers were through the wall. Dendrimers in aneurysmal aortas, on day 0 postdendrimer perfusion, diffused farther into the aortic wall than controls. In conclusion, this study documents labeled dendrimers delivered intra-arterially to native rat aortas in vivo , and the temporal diffusion of these molecules within the aortic wall. Increasing perfusion time and length of time prior to harvest resulted in continued dendrimer diffusion into the aortic wall. These preliminary data provide a novel mechanism whereby local inhibitory therapy may be delivered locally to aortic tissue. | en_US |
dc.format.extent | 323693 bytes | |
dc.format.extent | 3109 bytes | |
dc.format.mimetype | application/pdf | |
dc.format.mimetype | text/plain | |
dc.publisher | Blackwell Publishing Inc | en_US |
dc.rights | 2006 New York Academy of Sciences | en_US |
dc.subject.other | Aorta | en_US |
dc.subject.other | Aneurysm | en_US |
dc.subject.other | Dendrimer | en_US |
dc.subject.other | Drug Delivery | en_US |
dc.subject.other | Nanotechnology | en_US |
dc.subject.other | PAMAM | en_US |
dc.title | Diffusion of Alexa Fluor 488-Conjugated Dendrimers in Rat Aortic Tissue | en_US |
dc.type | Article | en_US |
dc.subject.hlbsecondlevel | Science (General) | en_US |
dc.subject.hlbtoplevel | Science | en_US |
dc.description.peerreviewed | Peer Reviewed | en_US |
dc.contributor.affiliationum | Department of Biomedical Engineering, University of Michigan, Ann Arbor, MI, USA | en_US |
dc.contributor.affiliationum | Department of Surgery, Jobst Vascular Research Laboratories, University of Michigan, Ann Arbor, MI, USA | en_US |
dc.contributor.affiliationum | Department of Internal Medicine, University of Michigan, Ann Arbor, MI, USA | en_US |
dc.identifier.pmid | 17182946 | en_US |
dc.description.bitstreamurl | http://deepblue.lib.umich.edu/bitstream/2027.42/72448/1/annals.1383.004.pdf | |
dc.identifier.doi | 10.1196/annals.1383.004 | en_US |
dc.identifier.source | Annals of the New York Academy of Sciences | en_US |
dc.identifier.citedreference | Brieger, D. & E. Topol. 1997. Local delivery systems and prevention of restenosis. Cardiovasc. Res. 35: 405 413. | en_US |
dc.identifier.citedreference | Sakharov, D.V., A.F. Jie, M.E. Bekkers, et al. 2001. Polylysine as a vehicle for extracellular matrix-targeted local drug delivery, providing high accumulation and long-term retention within the vascular wall. Arterioscler. Thromb. Vasc. Biol. 21: 943 948. | en_US |
dc.identifier.citedreference | Majoros, I.J., T.P. Thomas, C.B. Mehta & J.R. Baker. 2005. Poly(amidoamine) dendrimer-based multifunctional engineered nanodevice for cancer therapy. J. Med. Chem. 48: 5892 5899. | en_US |
dc.identifier.citedreference | Malik, N., R. Wiwattanapatapee, R. Klopsch, et al. 2000. Dendrimers: relationship between structure and biocompatibility in vitro, and preliminary studies on the biodistribution of 125I-labelled polyamidoamine dendrimers in vivo. J. Control Release. 65: 133 148. Erratum in: J. Control Release 2000. 68: 299 302. | en_US |
dc.identifier.citedreference | Patri, A.K., I.J. Majoros & J.R. Baker. 2002. Dendritic polymer macromolecular carriers for drug delivery. Curr. Opin. Chem. Biol. 6: 466 471. | en_US |
dc.identifier.citedreference | Lesniak, W., A.U. Bielinska, K. Sun, et al. 2005. Silver dendrimer nanocomposites as biomarkers: fabrication, characterization, in vitro toxicity, and intracellular detection. Nano. Lett. 5: 2123 2130. | en_US |
dc.identifier.citedreference | Bosman, A.W., H.M. Janssen & E.W. Meijer. 1999. About dendrimers: structure, physical properties, and applications. Chem. Rev. 99: 1665 1688. | en_US |
dc.identifier.citedreference | Ailawadi, G., J.L. Eliason & G.R. Upchurch Jr. 2003. Current concepts in the pathogenesis of abdominal aortic aneurysm. J. Vasc. Surg. 38: 584 588. | en_US |
dc.identifier.citedreference | Petrinec, D., S. Liao, D.R. Holmes, et al. 1996. Doxycycline inhibition of aneurysmal degeneration in an elastase-induced rat model of abdominal aortic aneurysm: preservation of aortic elastin associated with suppressed production of 92-kD gelatinase. J. Vasc. Surg. 23: 336 346. | en_US |
dc.identifier.citedreference | Sho, E., J. Chu, M. Sho, et al. 2004. Continuous periaortic infusion improves doxycycline efficacy in experimental aortic aneurysms. J. Vasc. Surg. 39: 1312 1321. | en_US |
dc.identifier.citedreference | Thompson, R.W., S. Liao & J.A. Curci. 1998. Therapeutic potential of tetracycline derivatives to suppress the growth of abdominal aortic aneurysms. Adv. Dent. Res. 12: 159 165. | en_US |
dc.identifier.citedreference | Curci, J.A., D. Petrinec, S. Liao, et al. 1998. Pharmacologic suppression of experimental abdominal aortic aneurysms: a comparison of doxycycline and four chemically modified tetracyclines. J. Vasc. Surg. 28: 1082 1093. | en_US |
dc.identifier.citedreference | Baxter, B.T., W.H. Pearce, E.A. Waltke, et al. 2002. Prolonged administration of doxycycline in patients with small asymptomatic abdominal aortic aneurysms: report of a prospective (phase II) multicenter study. J. Vasc. Surg. 36: 1 12. | en_US |
dc.identifier.citedreference | Anidjar, S., J.L. Salzmann, D. Gentric, et al. 1990. Elastase-induced experimental aneurysms in rats. Circulation 82: 973 981. | en_US |
dc.identifier.citedreference | Patri, A.K., J.F. Kukowska-Latallo & J.R. Baker. 2005. Targeted drug delivery with dendrimers: comparison of the release kinetics of covalently conjugated drug and non-covalent drug inclusion complex. Adv. Drug Deliv. Rev. 57: 2203 2214. | en_US |
dc.identifier.citedreference | Quintana, A., E. Raczka, L. Piehler, et al. 2002. Design and function of a dendrimer-based therapeutic nanodevice targeted to tumor cells through the folate receptor. Pharm. Res. 19: 1310 1316. | en_US |
dc.identifier.citedreference | Panchuk-Voloshina, N., R.P. Haugland, J. Bishop-Stewart, et al. 1999. Alexa dyes, a series of new fluorescent dyes that yield exceptionally bright, photostable conjugates. J. Histochem. Cytochem. 47: 1179 1188. | en_US |
dc.identifier.citedreference | Lovich, M.A. & E.R. Edelman. 1995. Mechanisms of transmural heparin transport in the rat abdominal aorta after local vascular delivery. Circ. Res. 77: 1143 1150. | en_US |
dc.identifier.citedreference | Price, R.J., D.M. Skyba, S. Kaul & T.C. Skalak. 1998. Delivery of colloidal particles and red blood cells to tissue through microvessel ruptures created by targeted microbubble destruction with ultrasound. Circulation 98: 1264 1267. | en_US |
dc.identifier.citedreference | Ailawadi, G., J.L. Eliason, K.J. Roelofs, et al. 2004. Gender differences in experimental aortic aneurysm formation. Arterioscler. Thromb. Vasc. Biol. 24: 2116 2122. | en_US |
dc.owningcollname | Interdisciplinary and Peer-Reviewed |
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