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Membrane perforations inhibit lysosome fusion by altering pH and calcium in Listeria monocytogenes vacuoles
dc.contributor.authorShaughnessy, Lee M.en_US
dc.contributor.authorHoppe, Adam D.en_US
dc.contributor.authorChristensen, Kenneth A.en_US
dc.contributor.authorSwanson, Joel A.en_US
dc.date.accessioned2010-06-01T19:31:07Z
dc.date.available2010-06-01T19:31:07Z
dc.date.issued2006-05en_US
dc.identifier.citationShaughnessy, Lee M.; Hoppe, Adam D.; Christensen, Kenneth A.; Swanson, Joel A. (2006). "Membrane perforations inhibit lysosome fusion by altering pH and calcium in Listeria monocytogenes vacuoles." Cellular Microbiology 8(5): 781-792. <http://hdl.handle.net/2027.42/72656>en_US
dc.identifier.issn1462-5814en_US
dc.identifier.issn1462-5822en_US
dc.identifier.urihttps://hdl.handle.net/2027.42/72656
dc.identifier.urihttp://www.ncbi.nlm.nih.gov/sites/entrez?cmd=retrieve&db=pubmed&list_uids=16611227&dopt=citationen_US
dc.description.abstractListeria monocytogenes ( Lm ) evade microbicidal defences inside macrophages by secreting a pore-forming cytolysin listeriolysin O (LLO), which allows Lm to escape vacuoles. LLO also inhibits Lm vacuole fusion with lysosomes, which indicates LLO alters vacuole chemistry prior to release of Lm into cytoplasm. Using fluorescent probes to measure membrane permeability, calcium and pH, we identified small membrane perforations in vacuoles containing wild-type but not LLO-deficient ( hly- ) Lm . The small membrane perforations released small fluorescent molecules and persisted for several minutes before expanding to allow exchange of larger fluorescent molecules. Macropinosomes and hly- Lm vacuoles acidified and increased their calcium content ([Ca 2+ ] vac ) within minutes of formation; however, the small perforations made by LLO-expressing bacteria increased vacuolar pH and decreased [Ca 2+ ] vac shortly after infection. Experimental increases in vacuolar pH inhibited Lm vacuole fusion with lysosomes. The timing of perforation indicated that LLO-dependent delays of Lm vacuole maturation result from disruption of ion gradients across vacuolar membranes.en_US
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dc.format.extent3109 bytes
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dc.publisherBlackwell Publishing Ltden_US
dc.rights© 2005 The Authors; Journal compilation © 2005 Blackwell Publishing Ltden_US
dc.titleMembrane perforations inhibit lysosome fusion by altering pH and calcium in Listeria monocytogenes vacuolesen_US
dc.typeArticleen_US
dc.subject.hlbsecondlevelMolecular, Cellular and Developmental Biologyen_US
dc.subject.hlbtoplevelHealth Sciencesen_US
dc.description.peerreviewedPeer Revieweden_US
dc.contributor.affiliationumDepartment of Microbiology and Immunology, University of Michigan Medical School, Ann Arbor, MI 48109-0620, USA.en_US
dc.contributor.affiliationotherDepartment of Chemistry, Clemson University, Clemson, SC 29634, USA.en_US
dc.identifier.pmid16611227en_US
dc.description.bitstreamurlhttp://deepblue.lib.umich.edu/bitstream/2027.42/72656/1/j.1462-5822.2005.00665.x.pdf
dc.identifier.doi10.1111/j.1462-5822.2005.00665.xen_US
dc.identifier.sourceCellular Microbiologyen_US
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dc.owningcollnameInterdisciplinary and Peer-Reviewed


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