The high-affinity phosphate transporter Pst is a virulence factor for Proteus mirabilis during complicated urinary tract infection
dc.contributor.author | Jacobsen, Sandra M. | en_US |
dc.contributor.author | Lane, Mary C. | en_US |
dc.contributor.author | Harro, Jean M. | en_US |
dc.contributor.author | Shirtliff, Mark E. | en_US |
dc.contributor.author | Mobley, Harry L. T. | en_US |
dc.date.accessioned | 2010-06-01T19:35:08Z | |
dc.date.available | 2010-06-01T19:35:08Z | |
dc.date.issued | 2008-03 | en_US |
dc.identifier.citation | Jacobsen, Sandra M.; Lane, Mary C.; Harro, Jean M.; Shirtliff, Mark E.; Mobley, Harry L. T. (2008). "The high-affinity phosphate transporter Pst is a virulence factor for Proteus mirabilis during complicated urinary tract infection." FEMS Immunology & Medical Microbiology 52(2): 180-193. <http://hdl.handle.net/2027.42/72722> | en_US |
dc.identifier.issn | 0928-8244 | en_US |
dc.identifier.issn | 1574-695X | en_US |
dc.identifier.uri | https://hdl.handle.net/2027.42/72722 | |
dc.identifier.uri | http://www.ncbi.nlm.nih.gov/sites/entrez?cmd=retrieve&db=pubmed&list_uids=18194341&dopt=citation | en_US |
dc.description.abstract | Proteus mirabilis is a ubiquitous bacterium associated with complicated urinary tract infection (UTI). Mutagenesis studies of the wild-type strain HI4320 in the CBA mouse model of ascending UTIs have identified attenuated mutants with transposon insertions in genes encoding the high-affinity phosphate transporter Pst ( pstS , pstA ). The transcription of the pst operon ( pstSCAB-phoU ) and other members of the phosphate regulon of Escherichia coli , including alkaline phosphatase (AP), are regulated by the two-component regulatory system PhoBR and are repressed until times of phosphate starvation. This normal suppression was relieved in pstS :Tn5 and pstA :Tn5 mutants, which constitutively produced AP regardless of growth conditions. No significant growth defects were observed in vitro for the pst mutants during the independent culture or coculture studies in rich broth, phosphate-limiting minimal salts medium, or human urine. Mutants complemented with the complete pst operon repressed AP synthesis in vitro and colonized the mouse bladder in numbers comparable to the wild-type strain HI4320. Therefore, the Pst transport system imparts a significant in vivo advantage to wild-type P. mirabilis that is not required for in vitro growth. Thus, the Pst transporter has satisfied molecular Koch's postulates as a virulence factor in the pathogenesis of urinary tract infection caused by P. mirabilis . | en_US |
dc.format.extent | 297665 bytes | |
dc.format.extent | 3109 bytes | |
dc.format.mimetype | application/pdf | |
dc.format.mimetype | text/plain | |
dc.publisher | Blackwell Publishing Ltd | en_US |
dc.rights | © 2008 Federation of European Microbiological Societies | en_US |
dc.subject.other | Proteus Mirabilis | en_US |
dc.subject.other | Urinary Tract Infection | en_US |
dc.subject.other | Virulence | en_US |
dc.subject.other | Phosphate | en_US |
dc.title | The high-affinity phosphate transporter Pst is a virulence factor for Proteus mirabilis during complicated urinary tract infection | en_US |
dc.type | Article | en_US |
dc.subject.hlbsecondlevel | Microbiology and Immunology | en_US |
dc.subject.hlbtoplevel | Health Sciences | en_US |
dc.description.peerreviewed | Peer Reviewed | en_US |
dc.contributor.affiliationum | Department of Microbiology and Immunology, University of Michigan Medical School, Ann Arbor, MI, USA ; and | en_US |
dc.contributor.affiliationother | Department of Microbiology and Immunology, School of Medicine, University of Maryland – Baltimore, Baltimore, MD, USA ; | en_US |
dc.contributor.affiliationother | Department of Biomedical Sciences, Dental School, University of Maryland – Baltimore, Baltimore, MD, USA | en_US |
dc.identifier.pmid | 18194341 | en_US |
dc.description.bitstreamurl | http://deepblue.lib.umich.edu/bitstream/2027.42/72722/1/j.1574-695X.2007.00358.x.pdf | |
dc.identifier.doi | 10.1111/j.1574-695X.2007.00358.x | en_US |
dc.identifier.source | FEMS Immunology & Medical Microbiology | en_US |
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dc.owningcollname | Interdisciplinary and Peer-Reviewed |
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